LOCUS       AZ048445                 726 bp    DNA     linear   GSS 18-DEC-2010
DEFINITION  PSB44 Barley PstI genomic clones Hordeum vulgare subsp. vulgare
            genomic clone PSB44, genomic survey sequence.
ACCESSION   AZ048445
VERSION     AZ048445.1
DBLINK      BioSample: SAMN00151886
KEYWORDS    GSS.
SOURCE      Hordeum vulgare subsp. vulgare (domesticated barley)
  ORGANISM  Hordeum vulgare subsp. vulgare
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Reeves,R.G., Pratchett,N. and Laurie,D.A.
  TITLE     Genomic sequences from a barley PstI library
  JOURNAL   Unpublished
COMMENT     Contact: Laurie DA
            Cereals Research Department
            John Innes Centre
            Norwich Research Park, Colney, Norwich NR4 7UH, UK
            Email: david.laurie@bbsrc.ac.uk
            Mapped on chromosome 5HS.
            PCR PRimers
            FORWARD: M13 Forward
            BACKWARD: M13 Reverse
            Insert Length: 726   Std Error: 0.00
            Class: shotgun.
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Hordeum vulgare subsp. vulgare"
                     /mol_type="genomic DNA"
                     /cultivar="Betzes"
                     /sub_species="vulgare"
                     /db_xref="taxon:112509"
                     /clone="PSB44"
                     /sex="hermaphrodite"
                     /tissue_type="Leaf"
                     /clone_lib="SAMN00151886 Barley PstI genomic clones"
                     /note="Vector: PUC18; Site_1: PstI; Site_2: PstI; Genomic
                     fragments from PstI digest of DNA extracted from leaves."
BASE COUNT          196 a          141 c          165 g          224 t
ORIGIN      
        1 ggacctttcc tgccacaccg ttgctacttc gatattatta ttatcttatt accatgctct
       61 ctagcttatt taccactgca ttgcaatgga cactttggat gtgcgttata tagttgtgcg
      121 agtgattgtg agtcaatctg gaagcctggt tgtttgctat cgttgtgaat cttgtggctg
      181 agtgttgtgg gtttggactg gattttcaga ttgtttggct ggtgcagttt catcctagtc
      241 acagttttac ttgttctaag aaagagggtc ttcagatctt gtgacacgca ttgctctgca
      301 aggtgctata ttcaaaataa atgtgatccc ccttggcact ttgttgtagt gtgctgcctt
      361 ccagatcctc caaaattgtt gataatttgt tagtgtctgc ttgggaagtt tggaaagatt
      421 ttgtagacgg aaaagtatag acctactagg agtctagaag tgtatctaaa acaatcccca
      481 ctataaaacc accattacaa ttgttcatgg ctgtgccctg ggaggctgag caacttcaca
      541 gtagcatgaa acaaccattg ggacatgggc ttcacaacag aagactacaa tctctactcg
      601 actaaaaaat ggaaataaaa atatattctt ggcgctctac tcaagcaagg ccatgatgca
      661 acaaggggaa gacaccaatg tcagctaaag aaagagggcg caaaagctgg gaaggctctt
      721 cttttg
//