LOCUS       AW743345                 485 bp    mRNA    linear   EST 06-JAN-2011
DEFINITION  up65g05.y1 Soares_mouse_NMIE Mus musculus cDNA clone IMAGE:2780984
            5', mRNA sequence.
ACCESSION   AW743345
VERSION     AW743345.1
DBLINK      BioSample: SAMN00158640
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 485)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Other_ESTs: up65g05.x1
            Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: -40RP from Gibco
            High quality sequence stop: 463.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C3H x 101 F1"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:2780984"
                     /sex="male"
                     /clone_lib="SAMN00158640 Soares_mouse_NMIE"
                     /dev_stage="newborn"
                     /lab_host="DH10B"
                     /note="Organ: inner ear, 170 pooled; Vector: pT7T3D-PacI;
                     Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed
                     with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCACACTTTTTTTTTTTTTTTTTTTTTTTT
                     3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library is
                     normalized, and was constructed and donated by Bento
                     Soares and M.Fatima Bonaldo (University of Iowa) and R.
                     Hardisty, A. Varela-Carver, P. Mburu and S.D.M. Brown (MRC
                     UK Mouse Genome Centre and Mammalian Genetics Unit,
                     Harwell, UK)."
BASE COUNT          115 a          133 c          110 g          127 t
ORIGIN      
        1 aattcggaac gaggaggcat actatgtttc ttgcctggtt tatgccatgc tggggatgaa
       61 aaccaggact tgggactgcc agtcaagcac tctgttgaca gactcacatc tgcaacccct
      121 aacagaagtt ttagctctga aaagcaaacc ctcccctgaa agttaccttg tggctccctg
      181 ataacctgac cagaagccag cccagcaaga ccgggtttcc tcttcagcag tcttgcatcc
      241 tgtcgttttc tccagcaaag ctgatgtgga aaaggctgga gtgggtgcta gtgtcctctg
      301 aaaatctgtc ttggatctgc cccaagggtc tgtgtttact ccacagccca ggatccttat
      361 ccattggcaa tgctccactg taaggccatt agctcgtaat tagtgccagc ttgtcggctt
      421 gtacctcaca aattgagctg ccacagagac tcctcactca gtccctttag gtctctcaat
      481 gacag
//