LOCUS AW743345 485 bp mRNA linear EST 06-JAN-2011 DEFINITION up65g05.y1 Soares_mouse_NMIE Mus musculus cDNA clone IMAGE:2780984 5', mRNA sequence. ACCESSION AW743345 VERSION AW743345.1 DBLINK BioSample: SAMN00158640 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 485) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Other_ESTs: up65g05.x1 Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -40RP from Gibco High quality sequence stop: 463. FEATURES Location/Qualifiers source 1..485 /organism="Mus musculus" /mol_type="mRNA" /strain="C3H x 101 F1" /db_xref="taxon:10090" /clone="IMAGE:2780984" /sex="male" /clone_lib="SAMN00158640 Soares_mouse_NMIE" /dev_stage="newborn" /lab_host="DH10B" /note="Organ: inner ear, 170 pooled; Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCACACTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed and donated by Bento Soares and M.Fatima Bonaldo (University of Iowa) and R. Hardisty, A. Varela-Carver, P. Mburu and S.D.M. Brown (MRC UK Mouse Genome Centre and Mammalian Genetics Unit, Harwell, UK)." BASE COUNT 115 a 133 c 110 g 127 t ORIGIN 1 aattcggaac gaggaggcat actatgtttc ttgcctggtt tatgccatgc tggggatgaa 61 aaccaggact tgggactgcc agtcaagcac tctgttgaca gactcacatc tgcaacccct 121 aacagaagtt ttagctctga aaagcaaacc ctcccctgaa agttaccttg tggctccctg 181 ataacctgac cagaagccag cccagcaaga ccgggtttcc tcttcagcag tcttgcatcc 241 tgtcgttttc tccagcaaag ctgatgtgga aaaggctgga gtgggtgcta gtgtcctctg 301 aaaatctgtc ttggatctgc cccaagggtc tgtgtttact ccacagccca ggatccttat 361 ccattggcaa tgctccactg taaggccatt agctcgtaat tagtgccagc ttgtcggctt 421 gtacctcaca aattgagctg ccacagagac tcctcactca gtccctttag gtctctcaat 481 gacag //