LOCUS AV373469 267 bp mRNA linear EST 06-SEP-2005 DEFINITION Mus musculus adult male colon cDNA, RIKEN full-length enriched library, clone:9030623N15, 3' end partial sequence, similar to ddbj:X00439 M.musculus thymocyte mRNA fragment for T-cell receptor pseudogene (86T3). ACCESSION AV373469 VERSION AV373469.1 KEYWORDS EST; 3'-end sequence (3'-EST). SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 267) AUTHORS Konno,H., Aizawa,K., Akahira,S., Akiyama,J., Carninci,P., Endo,T., Fukuda,S., Fukunishi,Y., Hara,A., Hayatsu,N., Hirozane,T., Hori,F., Ishii,Y., Ishikawa,T., Itoh,M., Izawa,M., Kadota,K., Kagawa,I., Kai,C., Kawai,J., Kikuchi,N., Kojima,Y., Koya,S., Kusakabe,M., Matsuyama,T., Miki,R., Mizuno,Y., Nakamura,M., Oda,H., Okazaki,Y., Owa,C., Ozawa,Y., Saito,H., Sano,M., Sato,K., Shibata,K., Shibata,Y., Shigemoto,Y., Shiraki,T., Sogabe,Y., Sugahara,Y., Suzuki,H., Suzuki,H., Takahashi,F., Tateno,M., Tominaga,N., Tsunoda,Y., Watahiki,A., Watanabe,S., Yamamura,T., Yasunishi,A., Yokota,T., Yoshiki,A., Yoshino,M., Muramatsu,M. and Hayashizaki,Y. TITLE Direct Submission JOURNAL Submitted (14-OCT-1999) to the DDBJ/EMBL/GenBank databases. Contact:Yoshihide Hayashizaki The Institute of Physical and Chemical Research (RIKEN), Omics Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan URL :http://www.osc.riken.jp/ REFERENCE 2 AUTHORS CONSRTM The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) TITLE The Transcriptional Landscape of the Mammalian Genome JOURNAL Science 309, 1559-1563 (2005) REFERENCE 3 AUTHORS CONSRTM RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium TITLE Antisense Transcription in the Mammalian Transcriptome JOURNAL Science 309, 1564-1566 (2005) REFERENCE 4 AUTHORS Carninci,P. and Hayashizaki,Y. TITLE High-efficiency full-length cDNA cloning JOURNAL Meth. Enzymol. 303, 19-44 (1999) REFERENCE 5 AUTHORS Sasaki,N., Izawa,M., Watahiki,M., Ozawa,K., Tanaka,T., Yoneda,Y., Matsuura,S., Carninci,P., Muramatsu,M., Okazaki,Y. and Hayashizaki,Y. TITLE Transcriptional sequencing: A method for DNA sequencing using RNA polymerase JOURNAL Proc. Natl. Acad. Sci. U.S.A. 95, 3455-3460 (1998) REFERENCE 6 AUTHORS Itoh,M., Kitsunai,T., Akiyama,J., Shibata,K., Izawa,M., Kawai,J., Tomaru,Y., Carninci,P., Shibata,Y., Ozawa,Y., Muramatsu,M., Okazaki,Y. and Hayashizaki,Y. TITLE Automated filtration-based high-throughput plasmid preparation system JOURNAL Genome Res. 9, 463-470 (1999) COMMENT Please visit our web site (http://www.osc.riken.jp/) for further details. FEATURES Location/Qualifiers source 1..267 /clone="9030623N15" /clone_lib="RIKEN full-length enriched" /db_xref="taxon:10090" /dev_stage="adult" /mol_type="mRNA" /note="cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. 1st strand cDNA was primed with a primer [5' GAGAGAGAGAAGGATCCAAGAGCTCTTTTTTTTTTTTTTTTVN 3'], cDNA was prepared by using trehalose thermo-activated reverse transcriptase and subsequently enriched for full-length by cap-trapper. cDNA went through one round of normalization to Rot = 10.0 and subtraction to Rot = 185.0. Second strand cDNA was prepared with the primer adapter of sequence [5' GAGAGAGAGATTCTCGAGTTAATTAAATTAATCCCCCCCCCCCCC 3']. cDNA was cloned into the XhoI and BamHI sites. Vector: a modified pBluescript KS(+) after bulk excision from Lambda FLC I. Cloning sites, 5' end: SalI; 3' end: BamHI. Host: DH10B." /organism="Mus musculus" /sex="male" /strain="C57BL/6J" /tissue_type="colon" BASE COUNT 89 a 41 c 35 g 102 t ORIGIN 1 acgtttcaaa aaataaacat tcttattttt tctatgctct gtttaaactt tcccttttaa 61 ttatcttcat tttttttaat gaatagctct ttcacaaaga attccagatt tttttaataa 121 gtgggagttt attaaaatta atttgtaacc agcttgtaga gccaataaag gcactaaatt 181 tttgatggac tattaaacag aaatgtaaac ctggaattcc ttttggcttc cttagccaga 241 ggaataaaac tggcaatcat tatagac //