LOCUS AP022344 6258 bp DNA circular BCT 23-JAN-2020 DEFINITION Enterococcus faecium KUHS13 plasmid pKO3 DNA, complete genome. ACCESSION AP022344 VERSION AP022344.1 DBLINK BioProject:PRJDB9240 Sequence Read Archive:DRR205461, DRR205462 BioSample:SAMD00202474 KEYWORDS . SOURCE Enterococcus faecium ORGANISM Enterococcus faecium Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus. REFERENCE 1 (bases 1 to 6258) AUTHORS Hashimoto,Y. and Tomita,H. TITLE Direct Submission JOURNAL Submitted (22-JAN-2020) to the DDBJ/EMBL/GenBank databases. Contact:Yusuke Hashimoto Gunma university graduate school of medicine, bacteriology; 3-39-22 Showa-machi, Maebashi, Gunma 3718511, Japan REFERENCE 2 AUTHORS Hashimoto,Y., Kita,I., Suzuki,M., Ohtaki,H. and Tomita,H. TITLE The single hospital outbreak of multispecies of vancomycin-resistant enterococci in Japan JOURNAL Unpublished (2020) COMMENT Annotated at DFAST https://dfast.nig.ac.jp/ ##Genome-Assembly-Data-START## Assembly Method :: Canu v. 1.8 Genome Coverage :: 100x Sequencing Technology :: Nanopore MinION; Illumina MiniSeq ##Genome-Assembly-Data-END## FEATURES Location/Qualifiers source 1..6258 /db_xref="taxon:1352" /host="Homo sapiens" /mol_type="genomic DNA" /organism="Enterococcus faecium" /plasmid="pKO3" /strain="KUHS13" CDS 1..951 /codon_start=1 /gene="rep" /inference="COORDINATES:ab initio prediction:MetaGeneAnnotator" /inference="similar to AA sequence:RefSeq:WP_000145511.1" /locus_tag="EfmKUHS13_31550" /product="replication initiation protein" /protein_id="BBU67537.1" /transl_table=11 /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ" CDS 972..1505 /codon_start=1 /inference="COORDINATES:ab initio prediction:MetaGeneAnnotator" /inference="similar to AA sequence:RefSeq:WP_002289340.1" /locus_tag="EfmKUHS13_31560" /product="DUF536 domain-containing protein" /protein_id="BBU67538.1" /transl_table=11 /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE TIDKQIDTEKKWWQLWK" CDS 1521..1691 /codon_start=1 /inference="COORDINATES:ab initio prediction:MetaGeneAnnotator" /inference="similar to AA sequence:RefSeq:WP_002292472.1" /locus_tag="EfmKUHS13_31570" /product="hypothetical protein" /protein_id="BBU67539.1" /transl_table=11 /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS PNEELFVKETDK" CDS 2141..2374 /codon_start=1 /inference="COORDINATES:ab initio prediction:MetaGeneAnnotator" /locus_tag="EfmKUHS13_31580" /product="hypothetical protein" /protein_id="BBU67540.1" /transl_table=11 /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV VIEYTEDGMVVTKYEDGYSELQDGAKRNIQILL" CDS 2440..2631 /codon_start=1 /inference="COORDINATES:ab initio prediction:MetaGeneAnnotator" /locus_tag="EfmKUHS13_31590" /product="hypothetical protein" /protein_id="BBU67541.1" /transl_table=11 /translation="MFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFY YQRGYDAAGRVVATRYSTL" CDS 2649..2846 /codon_start=1 /inference="COORDINATES:ab initio prediction:MetaGeneAnnotator" /locus_tag="EfmKUHS13_31600" /product="hypothetical protein" /protein_id="BBU67542.1" /transl_table=11 /translation="MISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQLY KSGLFLVLILVVLDIIKSKKK" CDS 3157..3546 /codon_start=1 /gene="mobC" /inference="COORDINATES:ab initio prediction:MetaGeneAnnotator" /inference="similar to AA sequence:RefSeq:WP_014748751.1" /locus_tag="EfmKUHS13_31610" /product="mobilization protein" /protein_id="BBU67543.1" /transl_table=11 /translation="MGLMSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSA EALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDV APDDAQRLSENLKIMQKELQKIWQQLK" CDS 3528..4442 /codon_start=1 /gene="mobA" /inference="COORDINATES:ab initio prediction:MetaGeneAnnotator" /inference="similar to AA sequence:RefSeq:WP_002289347.1" /locus_tag="EfmKUHS13_31620" /product="relaxase" /protein_id="BBU67544.1" /transl_table=11 /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP EQSEIRPTRRNHEQHVERGTDHQQELSL" CDS 4390..4680 /codon_start=1 /inference="COORDINATES:ab initio prediction:MetaGeneAnnotator" /inference="similar to AA sequence:RefSeq:WP_014748752.1" /locus_tag="EfmKUHS13_31630" /product="hypothetical protein" /protein_id="BBU67545.1" /transl_table=11 /translation="MNNTLNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQT TMDSQVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE" CDS 4687..5376 /codon_start=1 /inference="COORDINATES:ab initio prediction:MetaGeneAnnotator" /inference="similar to AA sequence:RefSeq:WP_002289349.1" /locus_tag="EfmKUHS13_31640" /product="hypothetical protein" /protein_id="BBU67546.1" /transl_table=11 /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES VKEWLDQQQQN" BASE COUNT 2310 a 1093 c 1166 g 1689 t ORIGIN 1 ttggcaaatg aaatcgtgaa ataccatcat gaattaaata ccattccgtt gcggaaattt 61 acaccagttg aaatgaatct atttttctct attgtgtcta gaatgcgcga aaaaggcgat 121 aaaacagtcc gtcttacttt tgaccaacta aaagatttaa gtaattataa agcaactgct 181 aataatcgtt tcattaatga tttagaaatg acttatgata aattaatgga tttacgtttt 241 ggtaaaagaa gtgctaatgg gctcgatata gatagattcg ttatgtttag tgagtttaaa 301 atacgtggaa ctgctaaaga tccatatgta gacatacaag tttacgaaaa agctttacct 361 ttactaaaca atttagatga gtgggttcgc tattcactcc aacaatttaa cgaattacaa 421 agtagctatg caaagactat gtttcgatta ctaaaacaat atcgaactaa aggttttgct 481 tatttttcta aagaggattt tcatgaacta ttagatattc caaaaagtta ctggaacaac 541 caagcaaacg ttgataatcg tgttataaag cctatacgac aggaattaac cgcaatcttc 601 aaaggattat caatcaaaaa gaaatacgga aaagggcgag gaaaacctgt cgttggctat 661 caatttacgt ttaaaccaga aatcaaaaaa gcagatgatt ttaataaaca tgcaccaacc 721 aaagaagaac aaaataaagc attggcttta gctgataaac aaaaagaata cgaagaacct 781 gctacaacaa ttaacgaaaa aatcaaacag aagaaacgag aacttgaact cctccaacaa 841 atcaaacaac tagaaaaaga gaaagaagct cttgaaaaac aaacagagtc cgcactttcg 901 aaaaaagaga aaaaaagttt attatctgat ttagataatt tattccagta gcaacctagg 961 aggcaaccag tatgcaacca caagaaacct ttacaataaa ggaattgtca gaacttttta 1021 agatgagtag gcaagcaata tcgaaacata tccaaaaatt agattcttcc atgatagcta 1081 aaaatgagcg tggttataag gttgtattgc gttctggagt attgcaactt gctcgcaact 1141 taggcaacca aatgttgcta gataggttgc acgagtacga acaagacaac gataaaaatg 1201 ttaatatacc aagcaaaaac gtggttacaa acaagttgct agatcagttg cgtgaagaaa 1261 atgaattttt acgagaacaa cttgccctca gagagcaaaa taattctcat atacaaaaac 1321 tactcgacca gcaacaacaa ctcaccctac aagccaacaa acaaatcgaa aaactgcaag 1381 aaaaactaca gttgacttat acagaagaag ctccagagaa tgactcaacg actctttctg 1441 aagcaacaga aacaatagac aaacaaattg atactgaaaa aaaatggtgg caactttgga 1501 aataactgga gggaacattc atgacaagaa aaaatcttct attcggttct attttactag 1561 gaattctagg attcagtccg atgttattgt cagtgatcaa taaagggaat aaacatggtg 1621 aattgaaatt gaatccaaat tattacaaca gtcctaacga agaattgttt gtaaaagaaa 1681 cagataaata ataaaaaaga ctgctctttc gagcagccag ttaaaaaata atgagtgttt 1741 attcttgaaa aagcaacgcg cttatgaagc gctaccaaaa atttatcata ttttattaaa 1801 aataacaacc cagtaaaaaa tgactggtaa aaattcacaa agaaacggaa agaagcaacc 1861 gcagaaaatc aagcttaaaa agagcttttg acctagacca atcgaaaatc acaaagtgag 1921 cctctgtatc ggttgataca agggctactt ggcatagtcg acattatgtc aacaagaatt 1981 taatataaca tattttccta atgcattcta actataccac attatcaaaa atataaatta 2041 tctttactaa ataaaatatt gacaagccta taccttttgt tttatgattt tcacaaagaa 2101 aacgttttct atatattttt ctgtatcagg aggaaattct atgaaaaaat ttactttagg 2161 ggtagctttg ttaactattg gggcagttgt tttaccgtca actactatag tttatgcttc 2221 agaaaattcc caagtggtat cattaaacga aaacaatgct actgaaaatg tagttattga 2281 gtacactgaa gatggtatgg tcgtaactaa atatgaggat ggttatagtg agttgcaaga 2341 tggggcaaaa aggaatatac aaatattgct gtaaccactg gagtagctgc taatgctatt 2401 aatgctgcct tttgggcagg agggaaagct agcacttcta tgtttggtat cccaggttgg 2461 gcaattggtg ctttgttaac aggtgcaagt tggactaaaa aaggtgcttc tccagggaat 2521 gcagttgcaa aagaatggga caaaaatggt aatggctgga ttggatttta ttaccaaagg 2581 ggatatgatg cagctggaag agttgtagct actagatatt caacgttata agttaaggag 2641 tgatgttttt gatatctaaa tcattaaaat cattaaaaac atttactaaa aagaatccgc 2701 aaatagctac tattcgtatt tttatatttg ctatagctgt tgctataatt gttaatatgc 2761 taagcggagg gcaattatac aaatctggtt tatttttagt gttaatctta gttgtcttag 2821 acataataaa gtctaaaaaa aaataaatcg atacatttta gaagaatagg gttgtttgaa 2881 tattacaaag caacaatttc accttcggtg aaataagcga aactttacaa aaagtggcta 2941 gcaaatgcta gcctttttgt attgtggagc gtggattttg agggggattc aagggggagc 3001 ttgcgctcct ttgacaagcg gttatttgca ccaaatacgg aggtatttgg ctagcaaata 3061 tagagcttgc caaacccttt tagtgagctt tactcattgt acctttttag gtacaatggt 3121 attgcgaacg atacgtccgt aggacgtact gaaagggtgg gacttatgag cgaacaacat 3181 caaaatttgg ctagcgatca ttctaaaaaa tatacctatc gatccgagcc aaaacaaatc 3241 agttttcgag tgagcgaatc cgaatttgca aagctgaagc agtcagctga agctttgcaa 3301 atgagtgtgc cggctttcgt gaaagccaag gcacaaaacg cgcgtctggt gacaccgaaa 3361 gtggcgccag acattgccca ggcaatcgcg cgcgatttag ccaaagctgg agggaatatc 3421 aatcaaattg ccaagtggtg caatacacac caacacgacg ttgctcctga tgatgctcaa 3481 cgtttatccg aaaacctaaa aatcatgcaa aaggagctac aaaagatatg gcaacagtta 3541 aagtaagtcg aaccacttca tgtagccgag caatcaatta cgcagaacca cgagcaaccg 3601 tcaaaacagg aataaactgt gacattgatt atgccaaaag tgaaatgaaa caaatacgca 3661 cgctttatgg aaaagacgat cgtgtacaag cacacttatt gattcagtcc tttcgaccag 3721 gagaaattac cgctgaaaaa gccaatcaac taggggcaga gtatgcgaaa aaaatagccc 3781 ctaaccacca aatcgcgatt tacacacata cagacaaaga ccatattcat aatcacattg 3841 tcattaactc cgttaatctt gaaactggga aaaaatttca agcccatggg caagcatttt 3901 taaacaaatg ttatgaccta aatgacgaga tttgtcttga acatgactta acaatcacgg 3961 aacgaggaaa gaaaaccgaa aaacgaacca tgtctgaaat taaattaaaa gaaaaaaatg 4021 aaccggtttg gaaagacgaa attcggtttg cgattgatca aacaatgaaa aacaaaaaaa 4081 ctcgaacgta tacccaattt tgtgattcac ttaaaacatt cggcattcat tgcttgaatc 4141 gcggaaaaaa ttttacttac gaattggtcc acaaaaagaa aaaagtacga tccaacaaac 4201 taggaaagga ttacgaaaag gagacgattt tacatgagct ggacagacga gaacgaacct 4261 acaatcaaca acgaaccaac gagctacgaa gagctcgagc tagcattcgc gaaactgcag 4321 aagaagtacg aagagaacaa cgaactttta cgcctgaaca atcggaaatt agaccgactc 4381 gaagaaacca tgaacaacac gttgaacgag gaacagacca ccaacaagaa ttatcgctct 4441 aaaacgttaa aagacatcga aaacgaaata aagagacaac gtgcctttct tgcgccttat 4501 acccaagaaa ggatacaaac tacgatggat tcacaagtca aaaaactcca ccaagcgcta 4561 ttagaccagc gaaaccaaga acaaaaaaag gctttaattc aacgatcgat ggatattctt 4621 gcccccttat gttgcttagt actaacatta ttagcctttt ggttggtatg gggagaataa 4681 acagacatgg cattctattt accctatcta ctcatttctg ccagtatttt aggaattatt 4741 tggcttagct ataaggctta tcaaacaaga tattcattaa aaggctctaa aattcgattt 4801 aagcgatttt tattggtcgg ggttatattt gccttatcaa tcgttttaag tggtttattc 4861 ggcgttttag agggtaataa acgtgtttca agaagcattt tattagggga tatcactcaa 4921 aagtatgaag cagcatgtga taaaaagaaa aaagagcaag cattagcaca aaaaatggag 4981 gaatttaccg cttgttatga ggacatgaac aatatttttg tggatcaaga gaaacgttta 5041 acagataaaa acatgaaaaa actcactcgt ttttatcaaa acttaccaga aaaacagcaa 5101 aaagaggtac aagaaaaata tgagcaagta aaaaaagacg tgcaatattt taaagatacg 5161 cagacagaag aagcttgttg taacttattt agcgatacaa tccctttttc aaccagtgaa 5221 caagaacgaa aagaacggca acaatcggtc acatacgaac gttatgaaac actgcttcag 5281 caagcaacaa acatacaaaa tccaacgaaa aaagaaacgg cattaaatta tctagaaagt 5341 gtcaaggaat ggttggatca gcaacaacaa aattgaattt gattagcaat catgttaaac 5401 tgaacctgca aatgatcaaa cattagatac acgaaagccc cagagaggtc ggattctctg 5461 gggctttttt tcaggttgat gtcgccttta ttgattttac ttcttgtttc ggttattcaa 5521 ccaataactg aataaagaga cacaaacttc tacaagaagg gggcaaatga accgttcaaa 5581 cattagatac acctccttcc agtcgcaaga ctgtcggata ggcgacaagt tattatacct 5641 tacaaaataa aatctgtgtg gtgtttttaa gtatccctta ttttcgggtt gttagggctt 5701 gccctgaccg cctgtaaggc gctttaagaa caagataagg ttttttaccc aagactgtct 5761 taaaacgact tagacgagct atcagaggct tcccccacct ccgttactga cttgttttta 5821 gcaaaggagc ctttaggcga ctgcgcagta caatgtgaga gcatagcgat cacacttttt 5881 tagcgaccgt agggagcaag gttttgaatt ggcagttttg aaaaatgcgc gaacctgttg 5941 gtagaaaaaa gaagtggttt ttaggcggga aattttgtca ccctctttta taatctcttt 6001 ttaaaccttt ttttaaacct ctttaccgtc ggctgtgtcc ttactccttc aatggataca 6061 gcgttttata aactacattt tgtctgcttt taaactacat tttgtctgct tttaaactac 6121 attttgtctg cttttaaact acattttgtc tgcttttaaa ctcttttttt gcactcacgt 6181 atgttataat atgcattatc acagacaaaa cgttgtttat aacgtctgta tccattgaag 6241 gagtaaggag gaatatct //