LOCUS AP017609 14428 bp DNA circular INV 28-SEP-2016
DEFINITION Ramazzottius varieornatus mitochondrial DNA, complete genome,
strain: YOKOZUNA-1.
ACCESSION AP017609
VERSION AP017609.1
DBLINK BioProject:PRJDB4588
Sequence Read Archive:DRR013908, DRR013909, DRR013910
BioSample:SAMD00054187
KEYWORDS .
SOURCE mitochondrion Ramazzottius varieornatus
ORGANISM Ramazzottius varieornatus
Eukaryota; Metazoa; Ecdysozoa; Tardigrada; Eutardigrada;
Parachela; Hypsibioidea; Ramazzottiidae; Ramazzottius.
REFERENCE 1 (bases 1 to 14428)
AUTHORS Kunieda,T., Yokobori,S. and Katayama,T.
TITLE Direct Submission
JOURNAL Submitted (25-JUL-2016) to the DDBJ/EMBL/GenBank databases.
Contact:Takekazu Kunieda
The University of Tokyo, Department of Biological Sciences,
Graduate School of Science; 7-3-1 Hongo, Bunkyo-ku, Tokyo
113-0033, Japan
URL :http://www.biol.s.u-tokyo.ac.jp/users/saibou/kuma/
REFERENCE 2
AUTHORS Hashimoto,T., Horikawa,D.D., Saito,Y., Kuwahara,H.,
Kozuka-Hata,H., Shin-I,T., Minakuchi,Y., Ohishi,K., Motoyama,A.,
Aizu,T., Enomoto,A., Kondo,K., Tanaka,S., Hara,Y., Koshikawa,S.,
Sagara,H., Miura,T., Yokobori,S., Miyagawa,K., Suzuki,Y., Kubo,T.,
Oyama,M., Kohara,Y., Fujiyama,A., Arakawa,K., Katayama,T.,
Toyoda,A. and Kunieda,T.
TITLE Extremotolerant tardigrade genome and improved radiotolerance of
human cultured cells by tardigrade-unique protein
JOURNAL Nat Commun 7, 12808 (2016)
REMARK Publication Status: Online-Only
DOI:10.1038/ncomms12808
COMMENT Project website
URL: http://kumamushi.org/
##Genome-Assembly-Data-START##
Assembly Method :: PCAP.REP assembler v. 06/07/05;
SOAPdenovo v. 1.3
Assembly Name :: Rvar_4.0
Genome Coverage :: 177.5x
Sequencing Technology :: Sanger: ABI 3730xl DNA Analyzer;
Illumina: Genome Analyzer IIx sequencer
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..14428
/db_xref="taxon:947166"
/mol_type="genomic DNA"
/note="contig: MT"
/organelle="mitochondrion"
/organism="Ramazzottius varieornatus"
/strain="YOKOZUNA-1"
CDS 1..1533
/codon_start=1
/gene="cox1"
/locus_tag="RvY_mtCox1"
/product="cytochrome c oxidase subunit 1"
/protein_id="BAV58161.1"
/transl_except=(pos:1..3,aa:Met)
/transl_table=5
/translation="MRWMFSTNHKDIGTLYFIFGIWAATVGSSLSMIIRSELSEPGSL
FAEEQLYNVTVTSHAFIMIFFFVMPILIGGFGNWLVPLMIGAPDMAFPRMNNLSFWLL
PPSFLLISTSTMAEQGAGTGWTVYPPLSNYFAHSGPAVDLTIFSLHIAGVSSILGAIN
FISTIINMRTPAMSMENMPLFVWSVLITAVLLLLALPVLAGAITMLLLDRNFNTSFFD
PAGGGDPILYQHLFWFFGHPEVYILILPGFGLVSQIIVHYSGKHLTFGHLGMIYAMST
IGLLGFIVWAHHMFTVGMDLDTRAYFTAATMIIAIPTGIKVFSWLSTIYGSKSLMHAP
MQWVLGFIFLFTLGGLTGIVLSNSSLDIVLHDTYYVVAHFHYVLSMGAVFAIIAGITH
WFPLFTGTQMNNKWLTAQFFSMFIGVNMTFFPQHFLGLAGMPRRYVDYPDTFYLWNNV
SSLGSILSIAATIFLFFIIWESLISQRKILFIASSNSVMEWKHNFPPVLHSYSQLSLI
NY"
CDS 1535..2216
/codon_start=1
/gene="cox2"
/locus_tag="RvY_mtCox2"
/note="TAA stop codon is completed by the addition of 3'
A residues to the mRNA"
/product="cytochrome c oxidase subunit 2"
/protein_id="BAV58162.1"
/transl_except=(pos:2216,aa:TERM)
/transl_table=5
/translation="MASWEQLNFQNSFSPLMEQFIFLHDHIMSILTLIMLIVGYSLSK
LCLSKFNVTKQSENHELEILWTFLPGVFLIIIAFPSLRLLYLSEESEKSPLNIKTMGH
QWYWSYEYSDFKDLEFDAFMSPVEKTTSFRLLETDNHTCIPTNTPIQMFVSSTDVLHS
WTIPAMGVKADSTPGRLNIINIIAMKPGLFYGQCSEICGTNHSFMPITLDTRPIFFFK
EWVKTMINS"
tRNA 2217..2279
/anticodon=(pos:2244..2246,aa:Lys,seq:ttt)
/gene="trnK(uuu)"
/locus_tag="RvY_mtTrnK"
/product="tRNA-Lys"
tRNA 2280..2344
/anticodon=(pos:2313..2315,aa:Asp,seq:gtc)
/gene="trnD(guc)"
/locus_tag="RvY_mtTrnD"
/product="tRNA-Asp"
CDS 2345..2503
/codon_start=1
/gene="atp8"
/locus_tag="RvY_mtAtp8"
/product="ATP synthase F0 subunit 8"
/protein_id="BAV58163.1"
/transl_table=5
/translation="MPHMSNLPWMSIYITMFVMMNLIFMSYSSPLHNSTNKKLSTKLL
SEKNKWLW"
CDS 2497..3156
/codon_start=1
/gene="atp6"
/locus_tag="RvY_mtAtp6"
/product="ATP synthase F0 subunit 6"
/protein_id="BAV58164.1"
/transl_table=5
/translation="MMTSLFSSFDPMSGFFSMNYLSFLTLLMSPICMDFMFLQKSRNK
FINLDMYASIESELKSTISNSFKKGKTNILMGVFFSLLLMNLAGLVPYVFTSTAHMTV
TLSMTLPFWLGFISFSLINNTNHFFSHLVPLSTPLPLSQFMVIIESVSQLIRPITLSV
RLAANMTAGHILMGLASSNVTMLNLSSSILIMLIILETAVAFIQSYVFTILISMYMNE
A"
CDS 3156..3939
/codon_start=1
/gene="cox3"
/locus_tag="RvY_mtCox3"
/note="TAA stop codon is completed by the addition of 3'
A residues to the mRNA"
/product="cytochrome c oxidase subunit 3"
/protein_id="BAV58165.1"
/transl_except=(pos:3939,aa:TERM)
/transl_table=5
/translation="MINQNHPFHLVFNSPWPIFTGSSVLTMLIGTVNWMCTKSPTMMN
MGLLLTIFNTSQWWRDVSRESSYQGYHSNLVMNGLRWGMILFITSEILFFFSFFWSFF
HSSLAPNLELGLMWPPLGIAAINPFQVPLLNTSVLLASGVFVTWAHHGLIEQNNKIMN
TNMMVTVVMGLYFTSLQAWEYWESSYSMSDSSFGSTFFLATGFHGLHVLVGTIFLLVS
LMRHNLNMYSLNHHLGLEASIWYWHFVDVVWLFLYSFLYWWSY"
tRNA 3940..4000
/anticodon=(pos:3970..3972,aa:Gly,seq:tcc)
/gene="trnG(ucc)"
/locus_tag="RvY_mtTrnG"
/product="tRNA-Gly"
CDS 4001..4343
/codon_start=1
/gene="nad3"
/locus_tag="RvY_mtNad3"
/note="TAA stop codon is completed by the addition of 3'
A residues to the mRNA"
/product="NADH dehydrogenase subunit 3"
/protein_id="BAV58166.1"
/transl_except=(pos:4343,aa:TERM)
/transl_table=5
/translation="MMIPTFSLIAIIIILMALSYVMKTQNRVKWQEKMSNFECGFDSN
HTNRMAFSLRFFIITVIFLVFDIEIAVILPSPMVEESLSIKSSTMINWSFFTLLLGGL
IFEWQQGALEWS"
tRNA 4344..4405
/anticodon=(pos:4375..4377,aa:Ala,seq:tgc)
/gene="trnA(ugc)"
/locus_tag="RvY_mtTrnA"
/product="tRNA-Ala"
tRNA 4406..4458
/anticodon=(pos:4424..4426,aa:Arg,seq:tcg)
/gene="trnR(ucg)"
/locus_tag="RvY_mtTrnR"
/product="tRNA-Arg"
tRNA 4465..4525
/anticodon=(pos:4493..4495,aa:Asn,seq:gtt)
/gene="trnN(guu)"
/locus_tag="RvY_mtTrnN"
/product="tRNA-Asn"
tRNA 4526..4582
/anticodon=(pos:4546..4548,aa:Ser,seq:gct)
/gene="trnS(gcu)"
/locus_tag="RvY_mtTrnSgcu"
/product="tRNA-Ser"
tRNA 4583..4644
/anticodon=(pos:4614..4616,aa:Glu,seq:ttc)
/gene="trnE(uuc)"
/locus_tag="RvY_mtTrnE"
/product="tRNA-Glu"
tRNA complement(4644..4706)
/anticodon=(pos:complement(4673..4675),aa:Phe,seq:gaa)
/gene="trnF(gaa)"
/locus_tag="RvY_mtTrnF"
/product="tRNA-Phe"
CDS complement(4707..6348)
/codon_start=1
/gene="nad5"
/locus_tag="RvY_mtNad5"
/note="TAA stop codon is completed by the addition of 3'
A residues to the mRNA"
/product="NADH dehydrogenase subunit 5"
/protein_id="BAV58167.1"
/transl_except=(pos:4707,aa:TERM)
/transl_table=5
/translation="MTLWLSGFFFVFFLIMTFFLFSMGVTVIEFQLFTSFFTSLSVSF
VIDFYSILFASVVSFISAMIFFFSQFYMDGDTQQKGFSLLLSGFVISMFFLIFSPNLF
FLLLGWDGLGLVSYLLVVYYMSVSSSVAGMLTFLMNRVGDIFFLFSLSLFSLFSLFSY
FDNKALVFVQMGAFFLLITFITKSAQIPFSSWLPAAMAAPTPVSSLVHSSTLVTAGVF
LLIRFSPLMLKFFYFLIVISVFTMLMAGTMANLEWDMKKIIAYSTLSQLSFMMLSYST
GLIIFCFFHLLTHALFKASLFMSGGLVIHSEDSSQDFRNMSPFSSGKKLFASSFVVCI
LSLCGFPFLSGFFSKDLILDTLLSSFVLIILFLIGVFMTILYSLRFSYYMLISLSSFR
SSLSGVYENVKYLILPIWSLIIFSIVMGSFWSEFFFNFFFFFTYLLSIWKLFYLMLVA
FLSLAYMFILKKIFFLKLYFFSFMWVLNQNMTARGVKLFTIMADKIFCFLDQSWWEFY
GPQNIFFSFTSASVLVMSFVEWSLVYFFALFIFLLFFFI"
tRNA complement(6349..6410)
/anticodon=(pos:complement(6376..6378),aa:His,seq:gtg)
/gene="trnH(gug)"
/locus_tag="RvY_mtTrnH"
/product="tRNA-His"
CDS complement(6414..7745)
/codon_start=1
/gene="nad4"
/locus_tag="RvY_mtNad4"
/product="NADH dehydrogenase subunit 4"
/protein_id="BAV58168.1"
/transl_table=5
/translation="MRYFSILILLIMLSLPLMMFSFLGAVDLAVLFVLFLFTIFTGGP
IFFISEILFFDKISFLLIILSFWLFSLMFLLGQKYKILKVSLMKYKMYMYILMSFIVF
SVMFSDLMAFYIFFEISLVPLVFIILGWGYQVERVQAFFYLFLYTMFGSLPLLFFFLL
TYSYLSLDWLMLYYLKNYSENFVLLLFLAMMIAFFIKLPLYGFHLWLPKAHVEAPVSG
SMILAAVLLKLSLYGFYRVSPLMKPLFFFFFFALVFLFWGALLSSLVCLRQTDMKSLI
AYSSVGHMGVMLGGFFFFSQMGLKAAIIIMMSHAFCSSALFYLVNFHYERSYSRQIFN
NRGQFIFFGMLMSFWFLFLAINFSAPPFLSLVGELGVFISSFGKDLTYMYVLGLASFV
VAAFCIYLFSVVIHGLGLKHYILQVEMDLNFFVLSYHFLPALLMVLLLDFF"
CDS complement(7724..7996)
/codon_start=1
/gene="nad4L"
/locus_tag="RvY_mtNad4L"
/product="NADH dehydrogenase subunit 4L"
/protein_id="BAV58169.1"
/transl_table=5
/translation="MSFLFSFMLIYMAFLFVLKFDHLLSIFISLEFLGLFLFMLLIFF
LNYFFLFTLFFLVITASESVLGLTLFVYFIRSSMKDYVYSFGILVF"
tRNA 7998..8060
/anticodon=(pos:8029..8031,aa:Thr,seq:tgt)
/gene="trnT(ugu)"
/locus_tag="RvY_mtTrnT"
/product="tRNA-Thr"
tRNA complement(8060..8122)
/anticodon=(pos:complement(8088..8090),aa:Pro,seq:tgg)
/gene="trnP(ugg)"
/locus_tag="RvY_mtTrnP"
/product="tRNA-Pro"
CDS 8124..8621
/codon_start=1
/gene="nad6"
/locus_tag="RvY_mtNad6"
/product="NADH dehydrogenase subunit 6"
/protein_id="BAV58170.1"
/transl_table=5
/translation="MKEIMIMSSITLWFFSLFQHPLILVGILTVQSAPAIYSALHASH
SGWMAFLLFLIFLGGLLIIFVYLSALIPNEIFLPSRMENASFSLLALLPILWSMKTIK
FSFFNHSQSPVSDFAHLFYHNMFTFMLCYLFISLTATMYITSKYKTPMKSSYYDKYTK
NPPPN"
CDS 8623..9675
/codon_start=1
/gene="cob"
/locus_tag="RvY_mtCob"
/product="cytochrome b"
/protein_id="BAV58171.1"
/transl_table=5
/translation="MMNSALVDLPAPSNITHFWNFGSLLGVCLMTQIITGIFLAMHYS
SSIDLAFDCISHLMRDVQGGWLMRVIHANGASLFFLAFFIHIGRGLYFKSYVLNMTWI
SGVTIFLMSMMTAFLGYVLPWGQMSFWGATVITNLLSAVPYIGTPLVEWVWGGFSVNN
ATLTRFFSLHYLLPFVISLLVIVHLTFLHEQKSSNPLGLRLGSDKITFHPYFSLKDLT
SLLLIMFMFMMINYTFPFSLMDAENFIPANPMVTPVHIQPEWYFLFAYAILRAIPNKL
GGVMALLLSILMLIPLILQSSMKQFNKENQKNMFFWFFVMIFLILTWLGMKPVEEPFL
SMSIVSTFFYFSWFFI"
tRNA 9677..9733
/anticodon=(pos:9695..9697,aa:Ser,seq:tga)
/gene="trnS(uga)"
/locus_tag="RvY_mtTrnSuga"
/product="tRNA-Ser"
CDS complement(9733..10650)
/codon_start=1
/gene="nad1"
/locus_tag="RvY_mtNad1"
/product="NADH dehydrogenase subunit 1"
/protein_id="BAV58172.1"
/transl_table=5
/translation="MLFMFDVSVYYFQVLIFVMVAVAFLTLVERKVLSYSQLRKGPNK
LGFVGLLQPFADALKLFSKEEMVPVSSNFLLYWFSPILGFVITMLLWVSLFSFSGYLD
FKFSLLFFLCCISLSVYGVIFSGWSSNSKYALMGGLRTVAQTVSYEITLAFIFLSLVF
FFVSYSFFEIYNFHTYFSFFFFLFPLAVVFFISCVVETNRAPFDLAEGESELVSGFNV
EYGGLKFALIFMAEYASILWMSLLCTFFLFLNFSFLFNFCFFSFFVFLFLIFRSSFPR
LRYDYLMYLTWKKFLPLVLIFFFFSFLLV"
tRNA complement(10651..10712)
/anticodon=(pos:complement(10681..10683),aa:Leu,seq:taa)
/gene="trnL(uaa)"
/locus_tag="RvY_mtTrnLuaa"
/product="tRNA-Leu"
tRNA complement(10716..10778)
/anticodon=(pos:complement(10748..10750),aa:Ile,seq:gat)
/gene="trnI(gau)"
/locus_tag="RvY_mtTrnI"
/product="tRNA-Ile"
tRNA complement(10779..10834)
/anticodon=(pos:complement(10803..10805),aa:Leu,seq:tag)
/gene="trnL(uag)"
/locus_tag="RvY_mtTrnLuag"
/product="tRNA-Leu"
rRNA complement(11224..12351)
/gene="rrnL"
/locus_tag="RvY_mtRrnL"
/product="large subunit ribosomal RNA"
tRNA complement(12352..12406)
/anticodon=(pos:complement(12386..12388),aa:Val,seq:tac)
/gene="trnV(uac)"
/locus_tag="RvY_mtTrnV"
/product="tRNA-Val"
rRNA complement(12407..13157)
/gene="rrnS"
/locus_tag="RvY_mtRrnS"
/product="small subunit ribosomal RNA"
tRNA complement(13158..13224)
/anticodon=(pos:complement(13193..13195),aa:Gln,seq:ttg)
/gene="trnQ(uug)"
/locus_tag="RvY_mtTrnQ"
/product="tRNA-Gln"
tRNA 13221..13282
/anticodon=(pos:13251..13253,aa:Met,seq:cat)
/gene="trnM(cau)"
/locus_tag="RvY_mtTrnM"
/product="tRNA-Met"
CDS 13283..14242
/codon_start=1
/gene="nad2"
/locus_tag="RvY_mtNad2"
/product="NADH dehydrogenase subunit 2"
/protein_id="BAV58173.1"
/transl_table=5
/translation="MNKNLPLMSMFYLISLMGMISSNNWFMFWIFMEMNSLCFIPLML
IKKTKKGGEASLLYFIVQGISSMFLLMAMINEESSLMDVSEPNSSLITVAFLKMGAGV
FFNWLFLLSFSITLSILLLILTLQKLSGFFLLLLSQAGLSYLMLIFIFLSSFMAMFIN
MKQNLFKSMLTVSSLSNTSWLLTAVVTTYSGWMFYFAVYSFSLVLSSTMNNKYLDQNY
RNSDTLENPLLFLSMGGLPPMPGFFPKILILMNLSYSRLSLLSIFLLLAACVDIFIYL
RFSFTSALKNNGNLMYCKAVTTKSIKMLSLSAVTMVTMMAIML"
tRNA 14244..14306
/anticodon=(pos:14274..14276,aa:Trp,seq:tca)
/gene="trnW(uca)"
/locus_tag="RvY_mtTrnW"
/product="tRNA-Trp"
tRNA complement(14305..14360)
/anticodon=(pos:complement(14336..14338),aa:Cys,seq:gca)
/gene="trnC(gca)"
/locus_tag="RvY_mtTrnC"
/product="tRNA-Cys"
tRNA complement(14364..14426)
/anticodon=(pos:complement(14394..14396),aa:Tyr,seq:gta)
/gene="trnY(gua)"
/locus_tag="RvY_mtTrnY"
/product="tRNA-Tyr"
BASE COUNT 5780 a 2364 c 1768 g 4516 t
ORIGIN
1 ctgcgatgaa tattttcaac aaaccataaa gatatcggaa ccctatattt tattttcggc
61 atttgagccg ccacagtggg aagatcactt agtataatta ttcgttccga acttagagaa
121 cccggctcac tgtttgccga agagcaactt tacaatgtca cagtaaccag acatgccttt
181 attataattt ttttttttgt tatacctatt ctgatcggag gctttggaaa ctgattagtc
241 ccacttataa ttggggcgcc ggatatagct ttcccacgaa taaataattt aagattttga
301 cttctgcccc cctcttttct attaatctca acaagcacaa tagctgaaca gggggcagga
361 acgggatgaa ctgtataccc gcctttatcc aattattttg ctcatagtgg ccctgccgta
421 gatttaacca ttttctctct ccatattgct ggcgtctctt caattttggg agcaatcaat
481 tttatttcca caatcattaa catgcgaaca cctgccataa gaatagaaaa cataccatta
541 tttgtgtgat cggttttaat tacagccgtt ttactgcttt tggcccttcc tgtactagct
601 ggtgctatta ccatactttt acttgaccga aattttaata cttctttttt cgatccagca
661 gggggagggg accctattct ataccaacac ctattttgat tttttggcca cccagaagtt
721 tatattttaa ttcttccggg gttcgggcta gtgtctcaaa ttattgtaca ttacagagga
781 aaacatctga ctttcgggca cctaggaata atttatgcca tgagaacaat tggtctatta
841 ggatttattg tatgagctca tcacatattt actgtaggta tagatctaga cactcgagct
901 tacttcacag ccgcaactat aattattgca attcctacag gtattaaagt tttcagatga
961 ttaagaacaa tttacggaag aaaatctcta atacacgccc ctatacaatg agtattgggt
1021 tttatttttc ttttcaccct tggaggatta acaggaattg tcttgtccaa ctcctcgtta
1081 gatattgttt tacatgatac ttactatgtc gtagctcact tccactatgt tctctcaata
1141 ggagctgttt ttgcaattat cgcgggaatt actcattgat tcccgctttt tacaggcacc
1201 caaataaata acaaatgatt aacagcgcag ttttttagaa tatttatcgg agttaatata
1261 acattttttc ctcaacattt tctaggacta gccgggatac ctcgacgata tgtagattac
1321 ccagatacgt tttatttgtg gaataatgtt tcttcattag ggtctatctt gtctatcgca
1381 gctacaattt ttttgttttt tattatttga gaaagcttaa tttcacaacg aaaaatttta
1441 tttatcgcaa gctctaatag agtaatagaa tgaaaacaca actttccgcc agtgttgcac
1501 tcatatagcc aactaagatt aattaattac taaaatggct agctgagagc aattaaattt
1561 tcaaaacaga ttttcacctc ttatagagca atttattttt ttgcatgacc atatcatatc
1621 tattctcaca ctaattatac ttattgttgg atacaggctg agtaaattat gccttagcaa
1681 atttaatgta actaaacaaa gagaaaatca cgaattagaa attttatgaa cttttttgcc
1741 aggagtattt ttaattatta tcgctttccc gtctttgcgt ttactttact tatcagagga
1801 aagagaaaaa tctcccttaa atattaagac aataggacac caatgatatt gatcatatga
1861 atacagagat tttaaagatc ttgaatttga tgcttttata agacctgtag aaaaaaccac
1921 ttcttttcgt ttgctagaaa cagataatca tacctgcatc ccaactaaca ctcccattca
1981 aatatttgta tcttcaacag atgttttaca ctcatgaacc attccagcta taggagtaaa
2041 agctgattcc actccaggac gactgaacat tatcaatatt attgcaataa aaccaggttt
2101 attctatgga cagtgttcag aaatttgcgg aacgaaccac agttttatac ccatcaccct
2161 tgacacacga ccaattttct ttttcaaaga atgagtaaaa acaataatta acagataatt
2221 ttaaagcttt aatgcatcga gcttttaatt cgaagacaca gaatagtctg tttaaattag
2281 aaaatttaat taaaaaaaaa taatataaag ttgtcaactt taaattacca gactgtaatt
2341 ttctataccc cacatgtcaa acttaccgtg aataagaatt tatatcacca tatttgttat
2401 gataaatcta atttttataa gttattcatc ccctctacat aatagtacca ataaaaaact
2461 ctcaacaaaa ttattgtctg aaaaaaacaa atgattatga taacaagact gttttcatcg
2521 tttgacccca taagaggttt tttttccata aattaccttt cttttttaac tttgctaata
2581 agaccgattt gtatagattt catattttta caaaaaagtc gaaacaaatt tatcaactta
2641 gatatatacg catcaattga aagagagtta aaaaggacaa tttcaaactc ttttaaaaaa
2701 ggaaaaacca atattcttat gggtgtattc tttagtttat tgcttataaa tttagcaggc
2761 ttagtacctt atgtattcac aagaacagct cacataactg taaccctatc aatgacacta
2821 ccattttgac tgggttttat ttcattcagt cttattaata acacaaacca tttttttagc
2881 catttagtgc cactaagcac cccccttccg ctctcacaat ttatggtaat catcgaaaga
2941 gtaagtcaac ttattcggcc aattacttta tcagtgcgat tagctgctaa tataacagct
3001 ggacacattt taataggact tgcaagtagc aacgtgacta tgttaaactt atctagcaga
3061 attcttatca tgcttattat tttagaaacg gcagtagctt ttattcaaag atatgtattc
3121 actattctaa tctcgatata cataaatgaa gcctaatgat caatcaaaat catccctttc
3181 atttagtttt taacagacca tgacctattt ttacgggaag aagtgtatta accatgctta
3241 tcggaactgt aaattggata tgtactaaat cccccacaat aataaacatg ggactcttac
3301 tgacaatttt taacacctcc caatgatgac gcgatgtttc acgggaaaga tcttaccaag
3361 gctatcatag gaacttagtc ataaatgggc ttcgatgagg aataatctta tttattactt
3421 ccgaaatttt gtttttcttt tcgttttttt gaaggttctt tcatagaaga ctagcgccca
3481 acttggaatt gggattaata tggccaccct taggaattgc agccattaac ccattccaag
3541 ttcctctttt aaatacttca gttcttctag caagaggagt ttttgtaact tgagctcatc
3601 acggcttaat tgaacagaat aataagatta taaatacaaa tataatagta acagtagtaa
3661 taggcttata cttcacttcc cttcaagctt gagaatactg agaatcatct tactcaataa
3721 gagattcaag atttggaaga actttctttc tagcgactgg atttcatgga cttcacgttt
3781 tagtgggaac aattttttta ttagtgtctc taatgcgaca caacctaaat atgtactctt
3841 taaaccatca tttaggctta gaagcttcta tctgatactg acattttgta gacgtagtct
3901 gattgttttt gtatagtttt ctttattgat gaagttatta cacttttaat atatttagta
3961 tatttgattt ccaatcaaaa agtttgactc aaacagtgta atcataattc ctacatttag
4021 acttattgca atcattatta tcttaatagc cctctcctat gtaataaaaa ctcaaaatcg
4081 ggttaaatga caggaaaaaa taagaaattt tgagtgcgga tttgattcta accacactaa
4141 ccgaatagcg ttttctttac gattttttat tattacagta atttttttag ttttcgatat
4201 cgaaattgct gtaattcttc cgagccccat agtagaagaa agtctttcca ttaaaagaag
4261 aacaataatt aattgaagat tttttacctt actattgggg ggcctaattt ttgaatggca
4321 gcaaggtgct ctagaatgat catgggagtg tatttaaaat aataaaattt aaattgcaat
4381 taaaaattgt caacgacctc acccaaaata agatatggta gtttcgacct acttttagat
4441 tcatttaatc cttatttatc ataataaatt gtgactgaaa cagtgtttag atgttaacta
4501 aaatcataag aaaacttcaa tttaaagaat ttaaaattta aagccgctaa ctttaataat
4561 tgtgcatagc atgaagattc ttaaataaat aatgtaaact aacatattat aatttcatta
4621 taaaaatgcc aaaggtttta ttttgtaaaa gaaaattact tttattagat tcttcaaatc
4681 taagctttaa tataagctac ttaaacagat gaaaaaaaat aataaaaaaa taaaaagtgc
4741 aaaaaaatat accaatcttc actcaacaaa agacatcaca agaacagaag ctgaagtaaa
4801 agaaaaaaaa atattttgag ggccataaaa ctctcaccaa ctctggtcta aaaaacaaaa
4861 aattttatca gctataatag taaataattt aacacctcga gccgtcatgt tttgatttaa
4921 aacccatata aaactaaaaa aatataattt taaaaaaaaa atttttttta agataaatat
4981 ataagccaaa gacaaaaatg ctactaatat caaataaaaa agttttcaaa tagataataa
5041 ataagtaaaa aaaaaaaaaa aattaaaaaa gaactctctt caaaaagaac ctataacaat
5101 agaaaaaata attaaactcc aaattggcaa aatcaaatac ttcacattct cgtacactcc
5161 agataaagat gaacgaaaag aagaaaggga aataagcata taataagaaa atcgtaacga
5221 atacaaaata gtcataaaca ccccaattaa aaataaaata ataagcacaa accttcttaa
5281 taaagtatct aaaattaaat ctttagaaaa aaacccagat aaaaagggaa acccgcacaa
5341 agacaaaatg caaactacaa aagaagaagc aaataatttt ttgccggaag aaaaaggaga
5401 tatattacga aaatcctgtc ttctgtcttc tctatgaata accagccccc ctcttataaa
5461 aagagaagct ttaaacaaag catgagttaa taaatgaaaa aaacaaaaga tgattaaacc
5521 agttgaataa gaaagtatta tgaaagaaag ttgtcttaaa gtagagtatg caataatttt
5581 tttcatatct cattctaaat tagctattgt cccagctatt aatatggtaa aaactgaaat
5641 aacaatgaga aaataaaaaa acttcaatat caaaggacta aaccgaatca gaagaaatac
5701 cccagccgtt acaagtgtag aagaatgaac taatgacgaa actggagtgg gggctgctat
5761 cgcagctgga agccaagacg aaaaaggaat ctgggctctc tttgtaataa aagtaattaa
5821 taaaaaaaaa gctcccattt gtacgaatac taaagcttta ttatcaaaat atgaaaataa
5881 tgaaaacaaa gaaaacaaac taagagaaaa aagaaaaaaa atatctccga ctcggtttat
5941 caaaaaagtc aatattcctg ctactgaact ggaaacactc atataataaa ccactaataa
6001 ataagaaact aagcccaaac cgtctcatcc aagaagtaaa aaaaataaat taggactaaa
6061 aattaaaaaa aacatagaaa taacaaaacc actcaacagc aaagaaaacc ccttttgctg
6121 agtatctccg tccatataaa attgagaaaa aaaaaaaatt atagcagaaa taaaactaac
6181 aactgaagca aacaaaatag aataaaaatc aattacaaaa gaaacagata aagaagtgaa
6241 aaaagaagta aacaattgaa attcgataac tgtgacccct atactaaata aaaaaaaagt
6301 cataataagg aaaaaaacaa aaaaaaaccc ccttagccat aaagtcaata ttagttggaa
6361 agccattttt gtaaccacaa tacaatattt tacacttaaa ttaaactaat actttaaaaa
6421 aaatctaata gcaagactat taataatgca ggcaaaaaat gatatgaaag aacaaaaaaa
6481 tttaaatcta tttcgacctg caaaatatag tgcttcaacc ccaatccgtg aatcaccact
6541 ctaaacaaat aaatacaaaa agctgccaca acaaaacttg ctaaccctaa aacatatata
6601 taagtcaaat ctttgccaaa agatctgata aaaacaccca actctccaac taatgacaga
6661 aaagggggag ctgaaaaatt aattgctaaa aacaaaaatc aaaaagacat aagtatccca
6721 aaaaaaatga actgaccccg gttattgaaa atttgacgag aataagatcg ctcataatga
6781 aaatttacta aataaaaaag agccctagaa caaaaagcat gagacattat aataataatt
6841 gcagctttta accctatttg tctaaaaaag aaaaaccccc caagcatcac tcctatatga
6901 ccaacggaag aataagcaat taaagatttc atatccgttt gtcgcaaaca tactaaacta
6961 cttaataaag cacctcaaaa taaaaaaacc aaagcaaaaa aaaaaaaaaa aaataaaggc
7021 tttattaaag gtgaaacacg ataaaatcca tacaatctta actttaataa aacagcagct
7081 aaaattatag accccgaaac aggagcttcg acatgagcct tagggagtca taaatgaaag
7141 ccgtaaagag gcaacttaat aaaaaaagca attattatag ctaaaaataa taataaaaca
7201 aaattttccc tatagttttt taaatagtat aatatgagcc aatctaacga taagtaactg
7261 taagttaaca aaaaaaaaaa tagcaaaggt aaagaaccaa acatagtata aagaaataaa
7321 taaaaaaaag cttgaacacg ttcaacctga tagccccacc ccaaaataat aaaaactaga
7381 ggcacaagag aaatttcaaa aaaaatgtaa aaagccatta aatctgaaaa catgactgaa
7441 aaaacaataa aacttatgag aatatatata tacattttgt attttatgag agacactttt
7501 aaaattttat acttttggcc caacaaaaat atcaaagaaa aaagccaaaa agatagaatg
7561 ataagcaaaa aagaaatttt atcaaaaaat aaaatttcag agataaaaaa aataggaccc
7621 ccagtaaaaa tggtaaacaa aaataaaaca aaaagcacag ccaaatcaac cgcacctaag
7681 aaagaaaata ttattaaagg caagcttaat ataattaata aaattaaaat actaaaatac
7741 cgaatctata aacataatct tttatagaag aacgaataaa ataaacaaaa agagttaaac
7801 ccaatacaga ttccctagct gtaataacta aaaaaaataa cgtgaacaaa aaaaaataat
7861 ttaaaaaaaa aattaaaagc ataaataaaa acagccccaa aaactctaaa gaaataaaaa
7921 tagaaagaag atgatcaaat tttaatacga aaagaaacgc catataaatc aatataaatg
7981 aaaaaagaaa agaaatagct aaaatagttt aaataaaaac gcagaccttg tatgtctaca
8041 atacttaaaa gtttttaact tcagaaagaa aatcacatct taaatctcca aaatttaaat
8101 tttatagtta aactatttcc gaaataaaag aaatcataat cataagaaga atcactttat
8161 gatttttcag ccttttccag caccctttaa ttctagtagg cattttaact gtccaaagcg
8221 ccccggcaat ttattccgct ctacacgctt cacacagggg atgaatagct tttttattat
8281 ttttaatttt tttaggggga ttgttaatta ttttcgttta tctttcagcc ctgattccta
8341 acgaaatttt tttaccctcc cgcatagaaa atgctagatt ttctctctta gcattgctgc
8401 caattttgtg atcaataaaa actattaaat ttagcttttt taaccataga cagaggccag
8461 taagagattt tgcccactta ttttatcata atatatttac atttatactg tgttatttat
8521 ttatttctct taccgcgaca atatatatca catccaaata taaaacacca atgaaaagat
8581 cttattatga caaatatacg aaaaacccac cccctaatta aaatcataaa ttctgctcta
8641 gtggatctac cagccccttc taatatcact cacttttgaa attttggatc tcttttaggg
8701 gtatgcttaa taactcaaat tattacaggc atttttttag caatacacta cagatcaaga
8761 attgacttag cttttgactg tattagacat ttaatacgag acgtgcaagg gggttgacta
8821 atgcgagtaa ttcacgccaa cggagcatca ctattttttt tagccttttt tattcatatt
8881 ggtcgaggat tatattttaa atcatatgtg ctgaatataa cttggatcag aggagtaact
8941 atttttttaa tatcaataat aacagcattt ttaggctatg tcttaccttg aggacaaata
9001 tctttttgag gagcaactgt aatcacaaac ttattatcgg cagtccccta tattggaaca
9061 cctttagtgg aatgagtgtg aggaggcttt tctgtaaata atgcaactct tactcgattt
9121 ttttcattgc actatttatt accttttgtc attagactat tagtaattgt tcatttaact
9181 tttctccacg aacaaaaatc cagcaaccca ttaggcctac gattggggag ggacaaaatc
9241 acttttcatc cttatttttc tttaaaagac ctaacgtcac tacttttaat tatattcata
9301 ttcataataa ttaactatac tttcccattt tctttaatag acgcagaaaa ttttattccc
9361 gcaaacccta tagtcacacc cgtgcacatc caaccggaat gatattttct attcgcctat
9421 gctattctac gcgcaattcc taataaatta ggaggtgtca tagcacttct actgtcaatc
9481 ttaatattaa ttcctttaat tttacaatca tcaataaaac aattcaacaa agaaaatcaa
9541 aaaaacatgt ttttttgatt ttttgttata atttttctaa tcctaacatg attaggaata
9601 aaacctgtag aagaaccttt tttaagaatg agaattgtaa gcacattttt ttatttcaga
9661 tgatttttca tttaaaaacc gctatctaat gttttgaaaa cataaaaaaa gtattaaata
9721 tacttatcgg ttttaaacta aaaggaaaga aaaaaaaaaa aaaataagaa ctaagggtaa
9781 aaactttttt caagttaaat atattaaata atcataacgc aaacgaggaa atgaagaacg
9841 aaaaattaaa aataaaaaaa caaaaaaaga aaaaaaacaa aaattgaata aaaaagaaaa
9901 gttcaaaaaa agaaaaaaag tacataaaag tctcattcat aaaatgctag catattcagc
9961 tataaaaatt aaagcaaact tcaaacctcc gtactctaca ttaaaaccgg agactagctc
10021 agattctccc tcagccaaat caaaaggagc tcgatttgtt tcaacaacac atgaaataaa
10081 aaaaactact gccaaaggaa aaagaaaaaa aaaaaatcta aaataagtat gaaaattata
10141 aatttcaaaa aaactgtatg aaacaaaaaa aaaaactaaa gacaaaaaaa taaaagcaag
10201 agtgatttcg taagaaacag tttgagcaac agtacgcaga ccccctatca aagcatattt
10261 agaattagaa gaccatcctg aaaagatcac gccataaact cttaaactaa tacaacacaa
10321 aaaaaataaa agagaaaatt taaaatcaag ataaccagaa aacctaaaca aagaaaccca
10381 taacagtata gtaataacaa aacccaaaat tggagaaaac caataaagca aaaaatttga
10441 agacacagga actatttctt ctttggaaaa caatttcaat gcatccgcaa aaggttgaag
10501 aagaccgaca aaaccaagtt tattgggacc tttacgaagt tgcctgtatc ttagcacctt
10561 acgttcaact agtgttaaaa aggcaacagc aactataaca aaaattaaaa cctgaaaata
10621 gtaaacagaa acatcaaata taaagagtat ttttagaaaa ataaaattta tatttgaatt
10681 ttaaattcaa agcactaatc tgccacacta aactacttta agaaaatgta aatttatata
10741 ctaccctatc aagatagtcc tttttcaggc atcttaaagt gtataaaata catttttaaa
10801 tcctaaattt aacacactaa tctgccaaca aaaattttta aaaaactata taaatataat
10861 aatataaaac atttttaatt acattttaaa tcgctacgcc caaccaagtt tcaatattaa
10921 attaattaat gaacaaataa ataagaaatt atataagaat aataaataaa taagaaaaga
10981 aacaaaaata agaataagag aaaaagcaaa tagaaaagat gacctcatac aaaataaagc
11041 aggcaagggc gaccccaaaa ggtcggttgt tctgaaactc ctttttccaa ttatatattg
11101 aagaaaacta ataataaata taatatattt attttactga cactttttaa aatctggccc
11161 aaagggcgcc tcaataaata gagacttttt tttttttttc ttaattttaa tatttaataa
11221 caattaaact aaataggagt cctttcgtac tatctatttt attttatttt gtcaaaaaag
11281 ataaagacca acctggctta caccgatttg aactcagatc atgaaaagtt ttatttgtcg
11341 aacagacaaa cttaaaaaag atctgcccct tcaagtgact ttaatccaac atcgaggtcg
11401 taaactttta aaacgatttg aactcttctt aaaaataacg ctgttaaccc caaggtaatt
11461 tatttttcat cactcataag tggatcttct tttttgccaa taaaaatttt ttttataaat
11521 gagatttcat taaccgcccc agttaaaata agtttatatg agaaaaacaa aagaattatt
11581 aaaactctac ggggtctttt cgtcttttta ttaataaaga gtattttcac tctacatcta
11641 acttcaactc aataaaaaaa ataaaaaatt ttttcaaaac gcctttcatt ccagtcccca
11701 ataaaaagac aaatgattat gctaccttag catagaaatc tacggccgtt aatatctcac
11761 cgggcagaca tcatactaaa ttatatctca gtactatgtt tttgcaaaac agatgaaaaa
11821 atacagtcaa attaatttta aatattttaa atgctattac acaccgagta aacaataatt
11881 ctactaattt tctcatagtt ttatagctat cgcctattaa attaaatttt tatttcaact
11941 acgataataa aataattagt aaattaataa gttataaaac tttacacttc taattttaat
12001 aaaatatcaa aaattttgct attgggaaaa gtaagataaa aatttaaact tttaagcttg
12061 catctgaaag aaaaaaaaat aaatttaaat ctaattaact agatacctat tgaatcaaga
12121 ctttttatca ctttttaatt ttaaattcaa attttccact ctgaaacggt aagaatccta
12181 ataattaaaa aaacttcaat aatcgagaaa caataaaccc aatcaaataa aaaaatcctt
12241 aaccccaagg cacgaccctt caattcattg tttataataa ttttcttttt taacaaaaat
12301 atgttgcttc tctttacata aaatttaaat tttaatatta ttgttttttc tttaaagcaa
12361 cccttgttaa ggataataat tcattgtaaa agaataaaat actttaaagg ctaatcttcc
12421 gattagccca ctttgttacg actttttttt cctaagggaa aaagtgacgg gcgatatgtg
12481 cacacccgga aaaaatattc aaaataaaaa tatatgtatt tttactttca aatccaatta
12541 tttttaaata tcctacttaa attaacaaaa aagaaattgt aaccaatctg acctttgcta
12601 taaactatat gtagatctga aaattaaagt taattcttta tttagtgaag ccaaaagcaa
12661 tatatcgacg acgatatata aattcaaata agcaaagtaa attcttgcgc gtactttctt
12721 ttagcagaca gctccctctg ataaaccaaa tgccgccaaa ttttttaaat tttaaatatt
12781 taaacctcac tcctttggct attacaaaaa aataacaagg tatctaatct tggtttttat
12841 gatttttata aaaaaaacta tttaaaaatc tcctataaga agaataattt tacttacaaa
12901 cctgtcctgc cgtaaattat attaattaat ttatctcaca taaaaacatc aaagtatata
12961 accgcagctg ctggcatact ataagcttga taaaaataat taaaatcttt tgtcaaaatt
13021 tatataaaat tttttagtac aacacaagct ctcaaaaaaa ttatgttcct aaattttcac
13081 tttatttttc aaaaccaata tcaaattttt tacatgttaa aatattataa aacaaataaa
13141 aaattaagta atattaatta ttataaaaat atttaaattt ttctttcaaa atcaaaattt
13201 gatataccaa tatattatat aataggataa gctaaacaag ctattgagtt catacctcaa
13261 cgataaaacc cttttcttat taatcaataa aaacttaccg cttatgagaa tattttattt
13321 aatcagacta ataggcataa ttagctcaaa taattgattt atattttgaa tttttataga
13381 aataaattcc ttatgcttta tcccattgat attaattaaa aaaacaaaaa aagggggtga
13441 agcttcttta ttgtacttta tcgtgcaagg aattagctca atatttttat tgatagccat
13501 aattaatgaa gaaagttcac taatagatgt atcagaacca aacagcagcc tcatcacagt
13561 agccttttta aaaatagggg caggggtttt ttttaattga ttattcttat taagcttttc
13621 cattacttta agaattttat tattaatcct caccctacag aaattaagag gattttttct
13681 tcttctttta agacaagccg gcttgtctta tttaatatta atctttattt ttctttcttc
13741 ttttatagct atattcatta atataaaaca aaatttattt aaaagaatgt tgacagtttc
13801 aagattgtcc aacaccagat gactcttgac cgcagttgta acaacatatt ctggatgaat
13861 attttatttt gcagtgtact cattttcttt agtattaagt tcaacaataa ataataagta
13921 tttagaccaa aattaccgta atagagacac gctagaaaac ccccttttat ttttaagcat
13981 aggaggactc ccccccatac caggattttt ccctaaaatt ttaattctga taaatttaag
14041 atattctcgg ttaagattgc taagaatttt tctccttctt gccgcgtgtg tagatatttt
14101 tatttattta cgattttcct tcacaagagc attgaaaaat aatggtaatc ttatatattg
14161 taaagctgta acaacaaaga gaattaaaat actgtcttta agagcagtca ctatagtcac
14221 aataatagca attatattat aaaaagattt taagttaaaa aaactaatgt gtttcaaaca
14281 cttcaataat gaaaatttaa atcttaattt taaagaaaaa ctttttaaat atgcttgcaa
14341 catatcatat aaataaaatt tcataacaaa aagaaaaaat ctatatgtaa atttacaatt
14401 taccgcctat actcagccat tttgacta
//