LOCUS AP013889 6959 bp DNA linear HTG 30-OCT-2015
DEFINITION Uncultured Mediterranean phage uvMED DNA, genomic fragment, group
G3, isolate: uvMED-CGF-C1B-MedDCM-OCT-S27-C229, *** SEQUENCING IN
PROGRESS ***.
ACCESSION AP013889
VERSION AP013889.1
DBLINK BioProject:PRJDB1484
KEYWORDS ENV; HTG; HTGS_PHASE2.
SOURCE uncultured Mediterranean phage uvMED
ORGANISM uncultured Mediterranean phage uvMED
Viruses; Duplodnaviria; Heunggongvirae; Uroviricota;
Caudoviricetes; environmental samples.
REFERENCE 1 (bases 1 to 6959)
AUTHORS Mizuno,C.M. and Ghai,R.
TITLE Direct Submission
JOURNAL Submitted (11-OCT-2013) to the DDBJ/EMBL/GenBank databases.
Contact:Rohit Ghai
Universidad Miguel Hernandez, Evolutionary Genomics Group,
Laboratorio de Microbiologia; Ctra. de Valencia, s/n, San Juan de
Alicante, Alicante 3550, Spain
REFERENCE 2
AUTHORS Mizuno,C.M., Rodriguez-Valera,F., Kimes,N.E. and Ghai,R.
TITLE Expanding the Marine Virosphere Using Metagenomics
JOURNAL PLoS Genet. 9, e1003987 (2013)
REMARK Publication Status: Online-Only
DOI:10.1371/journal.pgen.1003987
COMMENT URL:http://egg.umh.es
##Genome-Assembly-Data-START##
Assembly Method :: Velvet v. 1.2.03
Sequencing Technology :: Illumina HiSeq
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..6959
/clone_lib="MedDCM-OCT"
/collection_date="2007-10-15"
/country="Spain"
/db_xref="taxon:1407671"
/environmental_sample
/isolate="uvMED-CGF-C1B-MedDCM-OCT-S27-C229"
/isolation_source="metagenomic fosmid library constructed
from the 0.2-5um-size plankton collected from the deep
chlorophyll maximum in the Mediterranean Sea off the
coast of Alicante, Spain, 50m below sea surface"
/lat_lon="38.4 N 0.13 W"
/mol_type="genomic DNA"
/organism="uncultured Mediterranean phage uvMED"
CDS 1448..2032
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22741.1"
/transl_table=11
/translation="MGFISTKNKSAISAGSGGGYLNPSKIQSGGSVRFALLQDQPLEF
FECWGESSEGGVKPFRFSDDPSPEDIQEEMGPEFERRMNREGTAPEKVKFAIAVPVYN
YEASSVQIMQLSQKSLQNELDDISQMEDYANLLEWDFVMGKEGNGLETRYSLRPVPRK
KGSQDSIESAWTEARAAGFDIGRLLTGENPFKAE"
CDS 2192..2956
/codon_start=1
/product="KilA-N domain-containing protein"
/protein_id="BAR22742.1"
/transl_table=11
/translation="MTAIKPMYGQLQTRSWNGTAIPRRTSDGYINATAMAKASGKQWN
NYFQTDRTTTYMEALSRNLGIQVTSLYTAKPGEGTWIHERLAVDFARWISADFAVWMD
AWFLEEVKGLSQVQNTQPVLAPTVLDTVDRSINLLERLGGVDERAQMLLRDVVLNTAM
KSAGGTAPALEPAKELTLSEKLIELGCPSHKATPLAAKIGKEIKGLYRRKNGREPKSQ
DQLVNGRRCSVALYERDFLNWLDSDFQAFIKEFCAS"
CDS 2985..3146
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22743.1"
/transl_table=11
/translation="MPEVVTTVLDDGCISISVGDFTGVVSSMHLIEPKTHQLQSAWLA
REAELANAC"
CDS 3136..3936
/codon_start=1
/product="putative nuclease"
/protein_id="BAR22744.1"
/transl_table=11
/translation="MPADTQNALAGLRRWSLVRDDSGPHRVYRDESGVSYASVTHILK
ETSPQWQKDALDRWIQKPGSALERDIACQRGTLAHDHAEYVLKTAAKLARNSANKRGS
WRTGDDGLERAPKGITTWAIEKAIQGAPRVPWSASGYARGLRTWIGENVTAIHAIEFS
VHDPRGWAGTADALIDLNGTLCIADWKTSTNARSEDMLANYICQAGAYSLGLQTLTGL
KPKSGAVVVARRSGAPQVRLLSELELRGAECQWLERMDLWNSQQAHNN"
CDS 3885..4070
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22745.1"
/transl_table=11
/translation="MARENGLVEFPASPQQLEEALDRIFRGKVNVARQAEDLGMPVER
LKQLTTDYILERPMDRY"
CDS complement(4054..4350)
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22746.1"
/transl_table=11
/translation="MNEAKTLQAMAMPSWQPNEVRLVHLTFVSDAEGIQRCQTLAASL
DEAVKRARRHYGPGDLNAWGTRKLPIDGGLVGGGKLLRGKRDVDHTDSLINIYP"
CDS complement(4347..4538)
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22747.1"
/transl_table=11
/translation="MKLTQFTDHELALLADALYWKMNTQLKAGLGDSPRFKTLQELQD
RILSYLQIVDYDEPRKDFK"
CDS complement(4535..4750)
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22748.1"
/transl_table=11
/translation="MTYFPNRLVETAPADELWGPVTRSTRASAAGRMIKCPRCGAVHR
VYHFSWSALQCMSCDTMVDKFDWGRRV"
CDS complement(4747..4887)
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22749.1"
/transl_table=11
/translation="MNHDYDPADDLPFYTEEELEELAIEQEQLDWERSIPTPAERNRN
LR"
CDS complement(4884..5237)
/codon_start=1
/product="putative recombinase"
/protein_id="BAR22750.1"
/transl_table=11
/translation="MASQVEIQHRLSYARAMLERGIPVSSLATLLSARYFVSRSTAYE
DIRAAHQEIQLSDDGPAVDENPCDPVGVLAMLQHRFELAVATGDDKQTCQLIKAMDTA
KKWQGYKPQPVSPFT"
CDS complement(5237..5518)
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22751.1"
/transl_table=11
/translation="MNYATQTEQLHTFTNVPRVTINRWHITGHSNWDSTEKTTVQIAA
EHDAIPSAIVDFIAALHNTSYHSKEDIELLQRFAGAMGLDYPHIPGIKA"
CDS complement(5558..5947)
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22752.1"
/transl_table=11
/translation="MATLTELLTEAGSRWVDKDWTAPANRCIDDIIDRFDDCPEGLQD
FDRSDWVNLCECYTYQLLDRWNKQEKDVMAFFNDYCEAIGATSTLQALEGEADGFEDG
DDMNAAIVNHAMTWAARQLADEIYPDR"
CDS 6074..6511
/codon_start=1
/product="putative sigma factor"
/protein_id="BAR22753.1"
/transl_table=11
/translation="MADNNTEEKKTSVGDDESKRWRKGRGAAHKVEERAQVCYGYILE
GGTRHQIAEKVSSRFNVSMRTAHDDYKRAMELLRTEQTGTREELLNQLQALRLATVQR
ALKRGHFQTVATLLGDMGRVIGEAAPETVSLQVPELSIKIEDK"
BASE COUNT 1581 a 1889 c 1903 g 1586 t
ORIGIN
1 ctgatgtgct tacacagcgt ctgacacatt gccgacttgc ctgtgccgcc gtccgcgtga
61 atcagcagca accaaggctt cggcagcaag cccggaatca aataatcgaa cgaggcatca
121 tcaagctcgc tcacggccct gggacgaaac tctttgttgc gctcaaacgt gaggtgagca
181 tcaagcatcc ggtcaatggc cgccgcccct tctttatgcc gcccagcctc ttgggccagc
241 tgagttttgg cctggtcaag cagcgccggg ttttcgatac tgtcctctag ctccagcccc
301 cgcgagatga cttcctcacc gctgaggagg tccggcctgt acttaatcgg gagcgcctcg
361 atctcttcca ccagtcgtgc aataccgtcc ctttgaaatc gggtcttctg tgggtccacc
421 agatcggcct ggcggatcag cgacccaaat ccaaggccgc ccccacgaaa cccgttctcc
481 cagcgctcag cacacgggtt gtgaccatcc ttccaatcgt cggcgtactc gttatcgcgg
541 cggctccact cttcccacag cttcaagccg tcctcgttag gcagctcgct gtgaatcatc
601 gccccgattt cccaccagaa ctgctcactg ttggcaccac ggggctcgat gacactcaga
661 caactccgag caatctcaat cttctcctcc ctggaacggt tggagtaacg gctatcgcga
721 agtttgcggt tatcagttcg ttcgtgcagc tttctgtact gctcgcgcat ccgttccaaa
781 agccattcag gagcggtggg gattttattt acatcgccag taaaggcgta ctccccctca
841 tctttgtaag caccaaaaag cagaccttgc ctgccccaga gcacctccca accttcatgc
901 ccagcagcag cgtggctgag atcagaaaca atcagtcgat ccttatcagg aacaacaaac
961 agaaacttgg ccgcattctt tttcggtgag gtgatccgtg gagcgttttc gagatccttt
1021 ccccacttct cttcgatggc cccaaggttg gcgtccacgt caaatatgac gagcccacca
1081 gagcgagtgc cgctgtaaac accgacagcc gcaaaagtct ccggagcgtt ttcgatgtac
1141 tgagccgtca gctcagggga tagctcattg tgcgcggcac gcccaagcgg ggacttgcct
1201 tcagcttttt tgccgttagg catgacagcg cccttggcgt agatcggcgc agtggcccaa
1261 tgcttgggca ggctgcgaac gaaatcaacg agattcattt gctacagtgt ggtcgtggaa
1321 tgtttgactc atacccctgg gtgcttcacc ggctcccggg ggtttttcca ttctatagca
1381 gttgcaaacc ccgcacgctt tgctacattg atgaggcacc gggcatcgcg cctacagcaa
1441 aggacccatg ggcttcatct caaccaagaa caagtcagcc attagcgccg gttccggcgg
1501 cggctacctc aacccctcca aaatccagag cggcggcagc gtccgcttcg ccctcctgca
1561 agaccaaccc ctcgaattct tcgagtgctg gggcgagtca tctgaaggcg gcgtaaagcc
1621 cttccgtttc agcgacgatc ccagccctga ggacattcag gaagaaatgg gtcccgagtt
1681 tgagcgccgc atgaaccgcg agggcaccgc acccgagaag gtaaagttcg ccatcgctgt
1741 gccggtctac aactacgaag ccagcagcgt ccagatcatg cagctctctc aaaagagctt
1801 gcagaacgaa ctggacgaca tcagccagat ggaggactac gccaacctgt tggagtggga
1861 cttcgtgatg ggtaaggaag gcaacggctt ggaaactcgt tacagcctgc gccccgtgcc
1921 acgtaagaaa ggcagccaag acagcatcga atctgcctgg accgaagcac gcgccgctgg
1981 cttcgacatt ggccgcctgc tgaccggcga aaacccattc aaagccgagt aacacaaacc
2041 ggctactgac tcaggggtgc accatgtgtg tacccctttt ttattactta cttccggcat
2101 atttcggcat attcctgcat ataaccttgg ggcattagat cttggtaaga atgtcataca
2161 agctaaatct ctgtgataga ggtatagttt gatgaccgcc ataaagccga tgtacggaca
2221 gcttcaaacc cgctcctgga acggaaccgc aatcccccgc cgcacctctg atggctacat
2281 aaacgcgact gctatggcca aagccagcgg taagcagtgg aataactact ttcaaaccga
2341 tcgcactacc acctacatgg aggctttgtc aagaaacctg ggaatccagg taacttccct
2401 ttacaccgcc aagcctggag aggggacgtg gattcacgaa cgcttggcgg tggattttgc
2461 tcgttggatt agtgctgatt ttgcggtgtg gatggacgcc tggtttttgg aggaggtgaa
2521 gggcctatct caagtgcaaa acactcagcc tgttctggct cccacggtgc tggataccgt
2581 tgaccgcagc atcaatttgc tggagcgtct tggcggagta gacgagcggg ctcagatgtt
2641 gctgcgtgat gtcgtgctaa acactgcgat gaaatcagcg ggcggcactg ctcccgcact
2701 tgaacccgca aaggaactaa cgctttcaga aaagctgatt gagttgggct gcccttcgca
2761 caaagcaacc ccattagccg caaaaattgg caaggaaatt aaggggctgt accgaagaaa
2821 gaacggtagg gaacctaaaa gccaagatca gctagtcaat ggccgtcgtt gttctgttgc
2881 gctttatgag cgggacttct tgaactggct tgactccgat tttcaagcgt ttattaaaga
2941 gttctgcgcc tcgtaaaaac atgagtgacc acctggaacg ggccatgccc gaagtagtca
3001 caacagttct cgatgatggc tgtatttcca tttcagtggg agacttcact ggagtggtca
3061 gttcaatgca cctaatcgag cccaaaaccc atcaactaca gagtgcatgg ctggcccgtg
3121 aagctgagtt agccaatgcc tgctgataca cagaacgccc tcgccggact acgccgctgg
3181 tccctggtcc gtgatgactc tggcccccac cgcgtgtacc gagacgagtc cggcgtgtct
3241 tacgcctcag tgacccacat cctcaaagaa acctctccgc aatggcagaa agatgcactt
3301 gatcgatgga ttcaaaagcc aggctctgcc ctggagcgtg acattgcctg ccagcgcggg
3361 actcttgctc acgatcatgc ggagtacgtg ctcaagacag cagcgaaact cgcgcgtaat
3421 agcgctaaca agcgaggaag ctggaggact ggagatgacg gcctggaacg tgcccctaag
3481 gggatcacta cctgggccat cgagaaggcc attcaggggg ctcctagggt cccctggagc
3541 gcctctggct acgcccgagg tctacggact tggatcggag agaacgtaac cgctatccac
3601 gccatcgagt tctccgtgca tgacccacgc ggctgggctg gaacggctga cgccctcatc
3661 gacctcaacg gcacgctctg catcgctgac tggaagacca gcaccaacgc ccgcagcgag
3721 gatatgctgg ccaattacat ctgtcaggct ggagcgtatt ccctgggact ccagacgctg
3781 acagggctga agccaaagag cggcgctgtc gtggtggcac gtcgtagcgg agcaccgcag
3841 gtgcgcttgc tcagtgagtt agaattacgt ggggcggagt gccaatggct agagagaatg
3901 gacttgtgga attcccagca agcccacaac aactagaaga agccctggac cgtatcttca
3961 ggggcaaggt caatgtggct cggcaagccg aagacctagg aatgccggtg gagcggctaa
4021 agcagctaac caccgactac atcctggaac gtcctatgga tagatattga tcagactgtc
4081 cgtgtggtcc acgtcacgtt taccgcgtag cagcttcccc ccaccaacta aacccccatc
4141 tatgggtagt ttcctggtcc cccaggcatt gaggtcccct ggtccgtagt gtctcctggc
4201 acgcttcacg gcttcatcca gcgaagcagc cagagtttga catcgttgaa taccctcagc
4261 atcggataca aaagtgagat gcacgagtct cacctcgttg ggttgccacg agggcatggc
4321 cattgcctgg agcgtttttg cttcgttcat ttgaaatcct tacggggttc gtcgtaatca
4381 actatttgca agtaactgag tattcgatcc tgtaactcct ggagcgtttt aaacctggga
4441 ctgtctccca aaccagcttt aagctgcgtg ttcatcttcc aatacaaagc atcagccaac
4501 aaagctaatt cgtggtccgt gaattgagtt agcttcatac tcggcgaccc cagtcaaatt
4561 tatccaccat tgtgtcgcaa ctcatgcact ggagcgcaga ccacgagaag tggtagacgc
4621 gatgcaccgc accacagcgc ggacacttaa tcatgcgccc agcagcacta gccctggtgg
4681 aacgtgtcac cggaccccag agttcatctg ctggagcggt ttctacgaga cggtttggaa
4741 agtaagtcat cggaggttgc gattgcgttc tgcaggtgta gggatggagc gttcccaatc
4801 gagctgttct tgctcgatgg cgagttcttc gagttcttct tccgtataaa acggaagatc
4861 atctgctggg tcgtagtcgt ggttcatgtg aagggagaaa cgggttgtgg tttgtagccc
4921 tgccattttt tggcagtgtc catcgcttta atcagctggc acgtttgctt gtcgtcgcct
4981 gtggctacgg caagttcaaa cctgtgctgg agcatggcaa gcacaccgac agggtcacag
5041 gggttttcgt caacggctgg accgtcgtcc gaaagctgga tttcctgatg tgccgccctg
5101 atgtcttcgt aggccgtgga gcgtgagacg aagtagcgcg cgctcagcag cgtggctagc
5161 gatgagaccg gaatcccgcg ctcaagcatt gcgcgggcat agctcagccg gtgctggatt
5221 tctacttgtg acgccatcag gctttgatcc ctggaatgtg cggataatcc agccccatgg
5281 caccagcgaa gcgctgaaga agttcgatat cttctttgct gtggtagctg gtgttatgca
5341 gcgcagctat aaagtccacg atggcggacg ggatagcgtc gtgttcagct gctatctgga
5401 ccgttgtctt ctcggtgctg tcccagttgc tgtggcctgt gatgtgccag cggttgatgg
5461 ttacccgtgg cacgttggtg aaggtgtgaa gctgttcggt ttgtgtggcg tagttcatgg
5521 atggaacaaa atcggatttt ctggaaagtt ggacggatca gcggtcgggg taaatttcat
5581 cggctagctg gcgtgccgcc caggtcatcg catggttgac gatggcggcg ttcatgtcgt
5641 cgccgtcttc gaatccgtcg gcttctcctt cgagagcctg gagcgttgaa gttgcgccga
5701 tcgcttcgca gtaatcgttg aagaacgcca taacgtcctt ctcttgtttg ttccagcggt
5761 ccaagagttg gtatgtgtag cactcgcaaa gattgaccca atcggagcga tcgaaatctt
5821 gcaagccttc cgggcagtca tcgaaacggt cgatgatgtc gtcaatacag cgattggctg
5881 gagcggtcca gtctttgtca acccagcggg agcccgcttc ggtcagtagt tcggtcagtg
5941 tggccatggg cgagcctctt tgtgtgttgg actcctacag tgtagcacat tgtgggatgg
6001 tgtcaagcct ggatcgtcta gcgggatcag ctggtccggt tagtattagt gaagacttac
6061 taatccccag cccgtggctg ataacaacac cgaagagaag aagaccagcg tcggtgacga
6121 cgagtccaag cggtggcgca agggacgcgg tgcggcgcac aaagtagaag aacgcgcaca
6181 agtttgttat gggtacattc tggaaggtgg cacgagacat cagatcgctg agaaagtgtc
6241 ttcacggttc aatgtctcca tgcgtaccgc ccatgacgac tacaagcgcg caatggagct
6301 gctgagaacg gaacaaactg gcacgcgtga agaattgttg aaccaactcc aagcgttacg
6361 tctcgcaaca gtccaacggg cgcttaagcg cgggcacttc cagaccgttg ccacactgct
6421 gggcgacatg ggccgcgtca ttggcgaagc ggcaccagag accgtcagcc tgcaggtgcc
6481 ggaattgtct attaaaattg aagacaaata atagaactaa atgtagttaa ctcagcgccc
6541 ccagattaac acttggcagt agttgttaga tgaatgtttt gccatgcaat ctgtgtacgc
6601 tagttgatcc tgttcgttga aatgtttacc ggcaaagatt aacagtgaag cgagggaaac
6661 taaaccgaaa aaataaactt tggaagacat aactttgggt gggttagttt taacaacaga
6721 tcagttacta agtgttagta actctctgtt gctaagtata gcttagcaca ggcagattgc
6781 tgtgctactt tctctgtgcc actctctcag ctgtcacacg ctcccaaaat tctggttttt
6841 gggaaatttt ttcggatttt cggagttttc gggtctgtgt gccagtcggt gcaactgtca
6901 caggggggtg gggttgcaaa gtagtacact tgtacctaag cgcggggaac ttactgata
//