LOCUS AP013886 8116 bp DNA linear HTG 30-OCT-2015
DEFINITION Uncultured Mediterranean phage uvMED DNA, genomic fragment, group
G3, isolate: uvMED-CGF-C1B-MedDCM-OCT-S24-C185, *** SEQUENCING IN
PROGRESS ***.
ACCESSION AP013886
VERSION AP013886.1
DBLINK BioProject:PRJDB1484
KEYWORDS ENV; HTG; HTGS_PHASE2.
SOURCE uncultured Mediterranean phage uvMED
ORGANISM uncultured Mediterranean phage uvMED
Viruses; Duplodnaviria; Heunggongvirae; Uroviricota;
Caudoviricetes; environmental samples.
REFERENCE 1 (bases 1 to 8116)
AUTHORS Mizuno,C.M. and Ghai,R.
TITLE Direct Submission
JOURNAL Submitted (11-OCT-2013) to the DDBJ/EMBL/GenBank databases.
Contact:Rohit Ghai
Universidad Miguel Hernandez, Evolutionary Genomics Group,
Laboratorio de Microbiologia; Ctra. de Valencia, s/n, San Juan de
Alicante, Alicante 3550, Spain
REFERENCE 2
AUTHORS Mizuno,C.M., Rodriguez-Valera,F., Kimes,N.E. and Ghai,R.
TITLE Expanding the Marine Virosphere Using Metagenomics
JOURNAL PLoS Genet. 9, e1003987 (2013)
REMARK Publication Status: Online-Only
DOI:10.1371/journal.pgen.1003987
COMMENT URL:http://egg.umh.es
##Genome-Assembly-Data-START##
Assembly Method :: Velvet v. 1.2.03
Sequencing Technology :: Illumina HiSeq
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..8116
/clone_lib="MedDCM-OCT"
/collection_date="2007-10-15"
/country="Spain"
/db_xref="taxon:1407671"
/environmental_sample
/isolate="uvMED-CGF-C1B-MedDCM-OCT-S24-C185"
/isolation_source="metagenomic fosmid library constructed
from the 0.2-5um-size plankton collected from the deep
chlorophyll maximum in the Mediterranean Sea off the
coast of Alicante, Spain, 50m below sea surface"
/lat_lon="38.4 N 0.13 W"
/mol_type="genomic DNA"
/organism="uncultured Mediterranean phage uvMED"
CDS complement(325..486)
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22702.1"
/transl_table=11
/translation="MPEVVTTVLDDGCISISVGDFTGVVSSMHLIEPKTHQLQSAWLA
REAELANAC"
CDS complement(515..1279)
/codon_start=1
/product="KilA-N domain-containing protein"
/protein_id="BAR22703.1"
/transl_table=11
/translation="MTAIKPMYGQLQTRSWNGTAIPRRTSDGYINATAMAKASGKQWN
NYFQTDRTTTYMEALSRNLGIQVTSLYTAKPGEGTWIHERLAVDFARWISADFAVWMD
AWFLEEVKGLSQVQNTQPVLAPTVLDTVDRSINLLERLGGVDERAQMLLRDVVLNTAM
KSAGGTAPALEPAKELTLSEKLIELGCPSHKATPLAAKIGKEIKGLYRRKNGREPKSQ
DQLVNGRRCSVALYERDFLNWLDSDFQAFIKEFCAS"
CDS complement(1439..2023)
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22704.1"
/transl_table=11
/translation="MGFISTKNKSAISAGSGGGYLNPSKIQSGGSVRFALLQDQPLEF
FECWGESSEGGVKPFRFSDDPSPEDIQEEMGPEFERRMNREGTAPEKVKFAIAVPVYN
YEASSVQIMQLSQKSLQNELDDISQMEDYANLLEWDFVMGKEGNGLETRYSLRPVPRK
KGSQDSIESAWTEARAAGFDIGRLLTGENPFKAE"
CDS 2173..4584
/codon_start=1
/product="bifunctional DNA primase/polymerase"
/protein_id="BAR22705.1"
/transl_table=11
/translation="MNLVDFVRSLPKHWATAPIYAKGAVMPNGKKAEGKSPLGRAAHN
ELSPELTAQYIENAPETFAAVGVYSGTRSGGLVIFDVDANLGAIEEKWGKDLENAPRI
TSPKKNAAKFLFVVPDKDRLIVSDLSHAAAGHEGWEVLWGRQGLLFGAYKDEGEYAFT
GDVNKIPTAPEWLLERMREQYRKLHERTDNRKLRDSRYSNRSREEKIEIARSCLSVIE
PRGANSEQFWWEIGAMIHSELPNEDGLKLWEEWSRRDNEYADDWKDGHNPCAERWENG
FRGGGLGFGSLIRQADLVDPQKTRFQRDGIARLVEEIEALPIKYRPDLLSGEEVISRG
LELEDSIENPALLDQAKTQLAQEAGRHKEGAAAIDRMLDAHLTFERNKEFRPRAVSEL
DDASFDYLIPGLLPKPWLLLIHADGGTGKSAMCQTLCKHISQGRSFNVHGGLVNVPRG
RCLWLNGDQSERIVRRQFDLIGVTAGVDVVGEWDMSWYSRFKKLQGPKEAPNYDLVVI
DSLDGCNDSNPYEENRREYALPLKKLARRNGEDFGACTIIVIHHNNRNGSFRGTSAIR
AAVDETWNMQKLEGKQLAELGLPDNTRIVTVEKSRDDREGQEMAFALLSDYTYQIEPV
PEKDSKVNGPTRQMLDMLKEMRRTRIPWTVQDFVDHDLLGGVHKKRAIKYGLDKLEGQ
KLIERCATPASWKGKGRPPAFYEAVGTDVPGKFCSRAREKTVKPPVKSQTPSPGTDLN
NKADCQKSEIVKSPDEGLLTKPELLTKPIVGKNPSTATGQRFNADNQRHRGFDSDADD
WGMWD"
CDS 4828..5082
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22706.1"
/transl_table=11
/translation="MKRIEITVDDADADRLKLEADQLGISRSDLLRERIKCTPLKTDK
RLTPTDFYDLVERTRRRAGQGVDQRQVEGIVAFVFSELAG"
CDS 5144..5407
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22707.1"
/transl_table=11
/translation="MGHQVQFQYCKVRARAGVNALALVRFTSYDDEAKAVAVEQVTYQ
DDQVGHRDFEYQVAAALDCGIDVAVMTAYDLDYFPLVEALVSS"
CDS 5442..7280
/codon_start=1
/product="DNA polymerase"
/protein_id="BAR22708.1"
/transl_table=11
/translation="MSPQVFQGIEHLGKLATSQSLCFDTETLQLQPERGKLRLLQLGA
RDRDTIVLIDCFQLDKGDWADLRWFFSTPTRFWLAHNAVFDLGWLQEHNIYPAGWVRC
SMLASRLLTNGLPNSKHGLDSVVKRYLKDENGKPKELSKEQQRSDWSGDLSQEQLEYA
ANDVAALMELDPILEHRISRDRLGPAFKLECRALPAMAQMWRTGLPWNAENLQQRKID
YEHDIKGLAKDFVLHLDAAMPEEHKLPRDEDGSFNLRPKDQGKLRDGTKKYAGFNINS
PKQLVEKLTVVLGAAPRDANGKPSASRQALRSYAADHEVIQIYLEWKRCEKRRQMIES
IQEKMDGTGFVRASYMQLGAESGRMSCIKPNNQQIPRDKQFRSCVEAPEGWLLVDADF
GQMELRLAAAVADDERMIAAFQAGEDPHTVTAEAIGCDRQTAKSANFGLLYGSGATGL
RNYAGGMGITMTQERAADIRNEWLGVFQGVAKWQRDNAKEADETQDDKWAATRVPISG
MRRYLQGDMNRLTVRCNTPIQGAGAAILKCALGNLWPKVKEAGEDTVRIAAAVHDEIL
LLVRENAAQEWAATLKQVMEDAEAKWLGEIPALAEVSVGKTWSETH"
CDS 7356..7571
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22709.1"
/transl_table=11
/translation="MGRWMPRTGRQLVMERLNKAIQAATTGDLQRAAMFLERAREVRA
GCTNQRAQARRAQATAWKKKVDSSITW"
CDS 7596..7847
/codon_start=1
/product="hypothetical protein"
/protein_id="BAR22710.1"
/transl_table=11
/translation="MAIRHGNKQYFQILLDPNRAELVQEEALKKQQRATAWIREAVYA
ELKRVTSASVYSEAEAKDLARWRQSVRSQVEGRTKQNDD"
BASE COUNT 1877 a 2044 c 2384 g 1811 t
ORIGIN
1 cgttccaggc cgtcatctcc agtcctccag cttcctcgct tgttagcgct attacgcgcg
61 agtttcgctg ctgtcttgag cacgtactcc gcatgatcgt gagcaagagt cccgcgctgg
121 caggcaatgt cacgctccag ggcagagcct ggcttttgaa tccatcgatc aagtgcatct
181 ttctgccatt gcggagaggt ttctttgagg atgtgggtca ctgaggcgta agacacgccg
241 gactcgtctc ggtacacgcg gtgggggcca gagtcatcac ggaccaggga ccagcggcgt
301 agtccggcga gggcgttctg tgtatcagca ggcattggct aactcagctt cacgggccag
361 ccatgcactc tgtagttgat gggttttggg ctcgattagg tgcattgaac tgaccactcc
421 agtgaagtct cccactgaaa tggaaataca gccatcatcg agaactgttg tgactacttc
481 gggcatggcc cgttccaggt ggtcactcat gtttttacga ggcgcagaac tctttaataa
541 acgcttgaaa atcggagtca agccagttca agaagtcccg ctcataaagc gcaacagaac
601 aacgacggcc attgactagc tgatcttggc ttttaggttc cctaccgttc tttcttcggt
661 acagcccctt aatttccttg ccaatttttg cggctaatgg ggttgctttg tgcgaagggc
721 agcccaactc aatcagcttt tctgaaagcg ttagttcctt tgcgggttca agtgcgggag
781 cagtgccgcc cgctgatttc atcgcagtgt ttagcacgac atcacgcagc aacatctgag
841 cccgctcgtc tactccgcca agacgctcca gcaaattgat gctgcggtca acggtatcca
901 gcaccgtggg agccagaaca ggctgagtgt tttgcacttg agataggccc ttcacctcct
961 ccaaaaacca ggcgtccatc cacaccgcaa aatcagcact aatccaacga gcaaaatcca
1021 ccgccaagcg ttcgtgaatc cacgtcccct ctccaggctt ggcggtgtaa agggaagtta
1081 cctggattcc caggtttctt gacaaagcct ccatgtaggt ggtagtgcga tcggtttgaa
1141 agtagttatt ccactgctta ccgctggctt tggccatagc agtcgcgttt atgtagccat
1201 cagaggtgcg gcgggggatt gcggttccgt tccaggagcg ggtttgaagc tgtccgtaca
1261 tcggctttat ggcggtcatc aaactatacc tctatcacag agatttagct tgtatgacat
1321 tcttaccaag atctaatgcc ccaaggttat atgcaggaat atgccgaaat atgccggaag
1381 taagtaataa aaaaggggta cacacatggt gcacccctga gtcagtagcc ggtttgtgtt
1441 actcggcttt gaatgggttt tcgccggtca gcaggcggcc aatgtcgaag ccagcggcgc
1501 gtgcttcggt ccaggcagat tcgatgctgt cttggctgcc tttcttacgt ggcacggggc
1561 gcaggctgta acgagtttcc aagccgttgc cttccttacc catcacgaag tcccactcca
1621 acaggttggc gtagtcctcc atctggctga tgtcgtccag ttcgttctgc aagctctttt
1681 gagagagctg catgatctgg acgctgctgg cttcgtagtt gtagaccggc acagcgatgg
1741 cgaactttac cttctcgggt gcggtgccct cgcggttcat gcggcgctca aactcgggac
1801 ccatttcttc ctgaatgtcc tcagggctgg gatcgtcgct gaaacggaag ggctttacgc
1861 cgccttcaga tgactcgccc cagcactcga agaattcgag gggttggtct tgcaggaggg
1921 cgaagcggac gctgccgccg ctctggattt tggaggggtt gaggtagccg ccgccggaac
1981 cggcgctaat ggctgacttg ttcttggttg agatgaagcc catgggtcct ttgctgtagg
2041 cgcgatgccc ggtgcctcat caatgtagca aagcgtgcgg ggtttgcaac tgctatagaa
2101 tggaaaaacc cccgggagcc ggtgaagcac ccaggggtat gagtcaaaca ttccacgacc
2161 acactgtagc aaatgaatct cgttgatttc gttcgcagcc tgcccaagca ttgggccact
2221 gcgccgatct acgccaaggg cgctgtcatg cctaacggca aaaaagctga aggcaagtcc
2281 ccgcttgggc gtgccgcgca caatgagcta tcccctgagc tgacggctca gtacatcgaa
2341 aacgctccgg agacttttgc ggctgtcggt gtttacagcg gcactcgctc tggtgggctc
2401 gtcatatttg acgtggacgc caaccttggg gccatcgaag agaagtgggg aaaggatctc
2461 gaaaacgctc cacggatcac ctcaccgaaa aagaatgcgg ccaagtttct gtttgttgtt
2521 cctgataagg atcgactgat tgtttctgat ctcagccacg ctgctgctgg gcatgaaggt
2581 tgggaggtgc tctggggcag gcaaggtctg ctttttggtg cttacaaaga tgagggggag
2641 tacgccttta ctggcgatgt aaataaaatc cccaccgctc ctgaatggct tttggaacgg
2701 atgcgcgagc agtacagaaa gctgcacgaa cgaactgata accgcaaact tcgcgatagc
2761 cgttactcca accgttccag ggaggagaag attgagattg ctcggagttg tctgagtgtc
2821 atcgagcccc gtggtgccaa cagtgagcag ttctggtggg aaatcggggc gatgattcac
2881 agcgagctgc ctaacgagga cggcttgaag ctgtgggaag agtggagccg ccgcgataac
2941 gagtacgccg acgattggaa ggatggtcac aacccgtgtg ctgagcgctg ggagaacggg
3001 tttcgtgggg gcggccttgg atttgggtcg ctgatccgcc aggccgatct ggtggaccca
3061 cagaagaccc gatttcaaag ggacggtatt gcacgactgg tggaagagat cgaggcgctc
3121 ccgattaagt acaggccgga cctcctcagc ggtgaggaag tcatctcgcg ggggctggag
3181 ctagaggaca gtatcgaaaa cccggcgctg cttgaccagg ccaaaactca gctggcccaa
3241 gaggctgggc ggcataaaga aggggcggcg gccattgacc ggatgcttga tgctcacctc
3301 acgtttgagc gcaacaaaga gtttcgtccc agggccgtga gcgagcttga tgatgcctcg
3361 ttcgattatt tgattccggg cttgctgccg aagccttggt tgctgctgat tcacgcggac
3421 ggcggcacag gcaagtcggc aatgtgtcag acgctgtgta agcacatcag ccaggggcgg
3481 tcattcaacg ttcatggcgg tttggtcaac gtgcccaggg gccgctgcct ttggttgaac
3541 ggagaccaga gcgagcggat tgtgcggcgt cagtttgatc tgatcggtgt taccgctgga
3601 gttgatgttg tcggtgagtg ggacatgtcc tggtacagcc gcttcaagaa gctccagggt
3661 ccgaaggaag cgccgaacta tgacctcgtg gtgattgaca gcctggacgg ctgcaatgac
3721 tccaaccctt acgaggagaa ccgcagggag tacgcgctgc ccttgaagaa gcttgcacgg
3781 cgcaatggtg aggatttcgg ggcctgcacc atcatcgtga tccaccacaa caacaggaac
3841 ggcagtttcc gtggcactag cgccatcaga gcggccgtag acgagacgtg gaacatgcag
3901 aagctggagg gcaagcagct ggctgagttg ggcctgcctg acaacaccag gatcgtgact
3961 gttgaaaagt cacgcgatga ccgcgaagga caagagatgg cctttgcgct gctgtcggac
4021 tacacgtacc agatcgagcc ggtgccggag aaagacagca aggtcaacgg accaactcgg
4081 cagatgctgg acatgctcaa ggaaatgcgg cgtacgcgca taccttggac ggtccaggat
4141 tttgtggatc acgacctgct gggaggcgta cataagaagc gcgccattaa gtacggcttg
4201 gacaagctgg aggggcagaa gctcattgaa cgttgtgcta ccccagcgag ttggaagggt
4261 aaagggcggc ctcctgcgtt ctacgaggcg gttggtacag acgttccagg taagttctgc
4321 tctcgcgcgc gtgaaaagac cgtgaaaccc cctgttaaaa gtcaaactcc ttcccctgga
4381 acggatttaa acaacaaagc ggattgtcaa aagtccgaaa ttgtcaaaag tccggacgaa
4441 ggacttttaa caaaaccaga acttttaaca aagcctattg ttggtaagaa cccttccacc
4501 gcaacgggtc agcgttttaa cgcggataat cagagacata ggggtttcga ctctgacgcg
4561 gatgattggg gtatgtggga ctgacttact aggcaaaagc ttgtcaatgt gctactctgt
4621 gcacaggatt taccgggctg tgaccgaaaa acaaattcaa gccaccgtta gaacggctca
4681 ccattacggt gggtcgtttc tcagcaaagt tgctgaagct gccatgagcg cggacccacg
4741 caaccgaacc cgcgtcttag acgctttccc agaaatcgtg tctaagtacg gtccaggcag
4801 tgctttttac aacgaatatc tttgagcatg aaaaggattg aaatcacggt ggacgacgct
4861 gacgcggatc gtctgaagct tgaagctgac cagttgggca tttcacggtc tgacttgctc
4921 cgcgagcgaa ttaagtgcac tccccttaaa acggacaagc gtttaacgcc cactgatttt
4981 tacgatttgg tggagcgcac taggcgcaga gctggccagg gggtggacca gcgtcaggtt
5041 gaagggatcg ttgcgtttgt tttcagtgaa ctggctggct aggcagctgg ttcgtctgct
5101 catacgcaag cggggccata ctccctttaa gcgagtagcc cgcatgggac accaagttca
5161 gttccaatac tgcaaggtca gggctcgggc gggtgtaaat gctctagccc tggtgcgctt
5221 cactagctac gacgacgagg ctaaggctgt agctgttgag caagtgacgt accaggacga
5281 tcaggtaggg catcgagact ttgaatatca agtggctgct gccctcgatt gcgggattga
5341 tgtagccgtg atgacggcat acgatttgga ctattttccg ctggtagaag ccctggtatc
5401 ttcttgatgt gctacattaa gggagtcata cgacaccacc catgtctccc caagtttttc
5461 agggcattga gcacttaggc aagctcgcca cctctcagtc cctctgcttt gacacagaaa
5521 cgctccagct gcagcctgag cgtggcaagt tgcggctctt gcaattaggt gcccgagacc
5581 gcgacactat tgtccttatt gactgctttc aacttgacaa gggcgactgg gctgatctcc
5641 gctggttctt cagcactcca acccgattct ggctggccca taacgctgtc ttcgatcttg
5701 gttggctcca ggaacacaac atctatccgg ctgggtgggt ccgttgctcc atgctggcca
5761 gccgacttct cacaaacggc ctgcctaact cgaagcacgg tctcgacagc gttgtaaagc
5821 gctatctcaa agacgaaaac ggaaagccca aagagctgtc gaaagaacag cagcgttctg
5881 attggagcgg cgacctcagt caagagcagc tggagtacgc agctaatgat gttgcggctc
5941 tgatggagct agatcccatc ttggagcacc gcattagccg ggacaggctc ggaccagcct
6001 tcaagcttga gtgccgcgct ttacccgcga tggcgcagat gtggcgcaca ggtctgccct
6061 ggaacgctga gaacttgcag caacgcaaga tcgattacga gcacgacatc aaggggttgg
6121 ccaaagactt tgtgttacat cttgatgctg cgatgcctga agaacacaag ttgccgcgcg
6181 atgaggatgg cagttttaat ctgcgaccta aggatcaagg caagcttcgg gacggcacca
6241 aaaagtacgc aggcttcaat atcaatagcc ccaagcagct tgtggaaaaa cttactgtgg
6301 tacttggcgc tgccccgcgt gatgccaacg gcaagcccag tgcatccagg caggcactgc
6361 gttcttacgc cgcagatcac gaagtcatcc agatttacct ggagtggaaa aggtgtgaaa
6421 aacgccgtca gatgatcgag tcgattcaag aaaagatgga cggcactggc tttgtgcgtg
6481 ccagctacat gcagttgggg gcggagtcag ggcgcatgtc ctgcatcaag cccaacaatc
6541 agcagattcc gcgtgacaag cagttccgca gttgtgtcga agcgcctgaa ggttggctgc
6601 tagttgacgc agatttcggc cagatggagc tgcgacttgc tgcagcggtg gcagatgatg
6661 agcggatgat tgctgcgttc caggccgggg aggatccgca cactgtcact gcggaagcta
6721 tcggctgtga ccgtcagacc gcgaaatcag caaacttcgg tctgctgtat gggtctggcg
6781 ctacaggttt gcggaattac gcaggtggca tgggcatcac catgacccag gaacgggccg
6841 ctgatattag aaatgaatgg cttggtgtgt tccagggagt agcgaagtgg cagcgggaca
6901 acgcaaaaga agctgatgaa acgcaggacg ataaatgggc ggcaaccagg gttccgatct
6961 cagggatgag gcgttacctg caaggcgaca tgaaccggct gactgttcgc tgtaacaccc
7021 caatacaggg agctggtgct gccatcctta agtgcgcttt ggggaatctg tggccgaagg
7081 tcaaggaggc gggcgaagac accgtgcgta ttgctgctgc ggtgcatgac gaaattctgt
7141 tgcttgttag ggaaaatgca gctcaggagt gggcagcgac cttaaaacag gtgatggagg
7201 atgccgaggc caagtggctt ggagagattc ctgcgctggc tgaggtgtct gtaggtaaaa
7261 cctggagcga gactcactga tggtcaacgt ctactgcaca gatgaggggt ggttctgttc
7321 ctgtggagga cgggtaacgt accaccagga tcttcgtggg gcgatggatg ccgcgtacag
7381 gcaggcaact cgtgatggaa cgtttaaaca aggcgatcca ggcagcaacg acgggtgatt
7441 tgcaacgggc ggcgatgttc ttagagcggg cgcgcgaggt aagggcgggc tgtacgaatc
7501 agcgcgccca agctcgccgt gctcaagcca ctgcttggaa aaagaaggtc gattcgtcta
7561 ttacatggta acgtgcttgt agcacagtga aaactatggc tattcgccac ggaaacaaac
7621 agtactttca aatactgctt gacccgaacc gggctgagct agttcaagag gaagcgttga
7681 agaagcaaca gcgcgcaaca gcttggataa gagaagctgt atacgcagag ttgaagcggg
7741 ttacttcggc tagtgtctat agcgaggccg aagctaaaga cctggcgcgg tggcggcagt
7801 ccgttcgcag ccaggttgag ggtcggacca aacaaaacga cgactgattt tatttatgcg
7861 ctttgcactc aaagtggccg attctgagtc catgttttta tcggcttatt atgaaaaatc
7921 tggcgacact ttttggacta taaccgcctc agatgcttgc agttacaaca gttttaacca
7981 agctcaaact attctgagta agatacgtag acctttagaa attgtgccag tagcttttta
8041 gctacggcag actgtcagtc tcctgtaagg cgcacttttc tgcatggtac gcaccatcct
8101 gctgcatgtg tattac
//