LOCUS AK316014 3229 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA, FLJ78913 complete cds, highly similar to Rap
guanine nucleotide exchange factor 4.
ACCESSION AK316014
VERSION AK316014.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3229)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2008) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2008)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3229
/clone="BRAWH3029225"
/clone_lib="BRAWH3"
/db_xref="H-InvDB:HIT000499627"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 241..2817
/codon_start=1
/note="highly similar to Rap guanine nucleotide exchange
factor 4"
/protein_id="BAH14385.1"
/transl_table=1
/translation="MAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPANTITK
VPSEKILRAGKILRNAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSR
TQAVGMWQVLLEDGVLNHVDQEHHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQ
DTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGV
LIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGRLALVND
APRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRAS
NQGNSQPQQKYTVMSGTPEKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQ
LCPALVAHYHAQPSQGTEQEKMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMAFLE
EFYVSVSDDARMIAALKEQLPELEKIVKQISEDAKAPQKKHKVLLQQFNTGDERAQKR
QPIRGSDEVLFKVYCMDHTYTTIRVPVATSVKEVISAVADKLGSGEGLIIVKMSSGGE
KVVLKPNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFELMSSKDLA
YQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCS
QLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFY
AEFESLMDPSRNHRAYRLTVAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKM
RMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPR
RP"
BASE COUNT 963 a 712 c 750 g 804 t
ORIGIN
1 aaaagaaagg taggatgcat acgcagaaac attagtgcta atatgctgca gaaaagccaa
61 gataaatcag tccagctctc atacatcact aagggagaag ctgattgcaa cttgaccatg
121 tgcctgagta gtcccatcag tgagaagagg agaacatgga tttaaaaaaa actagaatgt
181 acccctaggg ctcctgccgt ggtggacctg ggagaagtgt ctggtaaata tcgacagtat
241 atggcaggac ttctggctcc tccttatggt gttatggaaa cgggctctaa caatgacagg
301 attcctgaca aggagaacac acctctcatt gaacctcacg ttcctcttcg tcctgctaac
361 accattacca aggtcccttc agagaagatc ctcagagctg gaaaaatttt acgaaatgcc
421 attctctctc gagcacctca catgataaga gatagaaaat accacctaaa gacatacaga
481 caatgctgtg tgggaactga actggtggac tggatgatgc agcagacacc atgtgttcac
541 tcccggactc aagctgttgg catgtggcaa gtcctgttag aagatggtgt tctcaaccac
601 gtggaccagg agcaccattt ccaagacaaa tatttattct atcgatttct ggatgatgag
661 cacgaggatg cccctttgcc tactgaggag gagaagaagg agtgtgatga ggagctccag
721 gacaccatgc tgctgctgtc acagatgggc cccgacgccc acatgaggat gatccttcgc
781 aaaccacctg gccagaggac tgtggatgac ctagagatta tctatgagga gcttcttcat
841 attaaagcct tatcccatct ttctaccaca gtaaaacgag agttagcagg tgttctcatt
901 tttgagtctc acgccaaagg agggactgtg ttgtttaacc agggggaaga aggtacctcc
961 tggtacatta ttctaaaagg atcagtgaat gtagtcattt acggcaaggg tgtggtctgc
1021 accctgcatg aaggagatga cttcggcagg ttagcactag tgaatgatgc cccacgagct
1081 gcctctatcg tcttacgaga agataactgc catttcttaa gagtagacaa ggaggatttc
1141 aaccggatcc taagggacgt ggaggcgaat acagtcagac ttaaagaaca tgaccaagat
1201 gtcttggtgc tggagaaggt cccagcaggg aacagagctt ctaatcaagg aaactcacag
1261 cctcagcaaa agtatactgt gatgtcagga acacctgaaa aaattttaga gcattttcta
1321 gaaacaatac gccttgaggc aactttaaat gaagcaacag attctgtttt aaatgacttt
1381 attatgatgc actgtgtttt tatgccaaat acccagcttt gcccggcact ggtggcccac
1441 taccacgcac agccttcaca aggtacagaa caggagaaaa tggattatgc cctcaacaat
1501 aagaggcgag tcatccgcct ggttctacag tgggctgcca tgtatggaga cctcctgcaa
1561 gaggatgacg tgtctatggc cttcctggag gagttttatg tatctgtatc agatgatgcc
1621 cggatgattg ctgccctcaa ggagcaactg ccagagttgg agaagattgt caagcaaatc
1681 tcagaagatg caaaggcacc acaaaagaag cacaaggttc ttttgcaaca gttcaatacg
1741 ggcgatgaga gagcccagaa gcgccagcct atccgcggct ctgatgaagt tctgtttaag
1801 gtctattgta tggaccacac ctacacaacc attcgggtgc cagtggccac ttcggtgaag
1861 gaagtcatca gtgcagttgc cgacaagctg ggctccgggg agggcctgat catagtcaag
1921 atgagttccg gaggagaaaa ggtggtgctc aaacctaatg atgtttcagt atttacgacg
1981 ctcaccatta atggacgcct gtttgcttgc ccgcgagagc aattcgattc actgactccc
2041 ttaccagaac aggaaggccc aactgttgga acagtgggaa cttttgaact gatgagctcc
2101 aaagatttag cataccagat gacaatttat gattgggaac tcttcaactg cgtgcatgag
2161 ctggagctaa tctatcacac atttggaagg cataatttta aaaagaccac agcaaacttg
2221 gatttgttcc tgaggagatt taatgaaatt cagttttggg tcgtcactga gatctgcctt
2281 tgttctcagc tcagcaagcg tgttcagcta ttaaaaaaat ttattaagat agcagcccac
2341 tgtaaggagt ataaaaatct gaattccttt tttgccatcg tcatgggact aagtaacgtt
2401 gctgtgagcc gcttggcact aacgtgggag aaactgccaa gcaagttcaa gaagttctat
2461 gcggagtttg aaagtttaat ggacccttca aggaaccaca gggcctacag gctgacagta
2521 gctaagctgg aacctcctct catccccttc atgcctttgc tcattaaaga tatgacattt
2581 actcatgagg ggaacaagac gttcattgac aatctagtaa actttgaaaa aatgcgcatg
2641 attgcaaata cggccagaac agtgagatac tacaggagcc aacccttcaa tcctgatgca
2701 gctcaagcta ataagaacca tcaggatgtc cggagttatg tacggcaatt aaatgtgatt
2761 gacaaccaga gaactttatc acagatgtca cacagattag agcctcgtcg accatagaca
2821 tttcaaatgc ccaaagcaac agtttgtctc cagtccacaa tctttcaaaa attccattta
2881 tgctactact gactgtattg ccactagaga attctacaaa acaagcaaaa acacatcctg
2941 agacacctca gggctgcatt cagcttacca gctacctagc aagagaagga attatttgac
3001 atgcctaaag cttacacact ctagcttagg aatccccagt ttgtggctac cctgtttcat
3061 ttccacttgt gccatcttct ttgctgaagt gttgaataag gcccacgtga agagtttggt
3121 agaaatagcc ttgtcaagag aactagcaag cccctgacat ttttttctaa gagtgtttat
3181 gggatgagat gtgctacaaa tgggatagat ttggataaaa ttttaactc
//