LOCUS AK299032 4255 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ51634 complete cds, highly similar to
Integrator complex subunit 2.
ACCESSION AK299032
VERSION AK299032.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4255)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..4255
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RI3000210"
/clone_lib="NT2RI3"
/db_xref="H-InvDB:HIT000493656"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells treated
2-weeks mitotic inhibitor after 5-weeks retinoic acid
(RA) induction."
/note="majorly NT2 neuron"
/organism="Homo sapiens"
CDS 357..3947
/codon_start=1
/note="highly similar to Integrator complex subunit 2"
/protein_id="BAG61107.1"
/transl_table=1
/translation="MTECTSLQFVSPFAFEAMQKVDVVCLASLSDPELRLLLPCLVRM
ALCAPADQSQSWAQDKKLILRLLSGVEAVNSIVALLSVDFHALEQDASKEQQLRHKLG
GGSGESILVSQLQHGLTLEFEHSDSPRRLRLVLSELLAIMNKVSESNGEFFFKSSELF
ESPVYLEEAADVLCILQAELPSLLPIVDVAEALLHVRNGAWFLCLLVANVPDSFNEVC
RGLIKNGERQDEESLGGRRRTDALRFLCKMNPSQALKVRGMVVEECHLPGLGVALTLD
HTKNEACEDGVSDLVCFVSGLLLGTNAKVRTWFGTFIRNGQQRKRETSSSVLWQMRRQ
LLLELMGILPTVRSTRIVEEADVDMEPNVSVYSGLKEEHVVKASALLRLYCALMGIAG
LKPTEEEAEQLLQLMTSRPPATPAGVRFVSLSFCMLQAFSTLVSTPEQEQLMVVWLSW
MIKEEAYFESTSGVSASFGEMLLLVAMYFHSNQLSAIIDLVCSTLGMKIVIKPSSLSR
MKTIFTQEIFTEQVVTAHAVRVPVTSNLSANITGFLPIHCIYQLLRSRSFTKHKVSIK
DWIYRQLCETSTPLHPQLLPLIDVYINSILTPASKSNPEATNQPVTEQEILNIFQGVI
GGDNIRLNQRFSITAQLLVLYYILSYEEALLANTKTLAAMQRKPKSYSSSLMDQIPIK
FLIRQAQGLQQELGGLHSALLRLLATNYPHLCIVDDWICEEEITGTDALLRRMLLTNN
AKNHSPKQLQEAFSAVPVNHTQVMQIIEHLTLLSASELIPYAEVLTSNMSQLLNSGVP
RRILQTVNKLWMVLNTVMPRRLWVMTVNALQPSIKFVRQQKYTQNDLMIDPLIVLRCD
QRVHRCPPLMDITLHMLNGYLLASKAYLSAHLKETEQDRPSQNNTIGLVGQTDAPEVT
REELKNALLAAQDSAAVQILLEICLPTEEEKANGVNPDSLLRNVQSVITTSAPNKGME
EGEDNLLCILREVQCLICCLLHQMYIADPNIAKLVHFQGYPCELLPLTVAGIPSMHIC
LDFIPELIAQPELEKQIFAIQLLSHLCIQYALPKSLSVARLAVNVMGTLLTVLTQAKR
YALFMPTLPSLVSFCRAFPPLYEDIMSLLIQIGQVCASDVATQTRDIDPIITRLQQIK
EKPSGWSQICKDSSYKNGSRDTGSMDPDVQLCHCIERTVIEIINMSVSGI"
BASE COUNT 1143 a 877 c 976 g 1259 t
ORIGIN
1 cttcctctca gaccagtacg gtggccgacg ggagtcagac gctggggatg aatgaaggtg
61 ctgggtgcag gttgaaaaag tctcccgctt ttccgttcct acagtcccgg ttctgccttt
121 gtgtggtgcc cccatccttc ccactttgta tcgacaaccc ggttggtccc ggcgtctgag
181 ttcttggtgt ccgagtcgac tcgaggcaca actagggttt ggggttccgg atatcgccta
241 ggcccaacat cggaccgcgc tctcgatttc tgccgcgtcc cgcctctagg acgcggagtc
301 cgtgtgcggt tccgtgaggc tggagggtag atcttaagga tcaacaaaca gtaataatga
361 ctgaatgtac aagtcttcag tttgtcagcc cttttgcttt tgaggcaatg cagaaggtgg
421 atgttgtttg cctggcatct ttaagtgatc cagaattaag acttcttctg ccctgtttgg
481 tacggatggc actttgtgca cctgctgacc agagccaaag ctgggctcag gataagaaac
541 tcatccttcg ccttctttct ggagtggaag ctgtcaactc cattgttgca ttgttgtccg
601 tggactttca tgctttagaa caagatgcca gcaaagaaca gcagcttagg cataaacttg
661 gaggaggcag tggagagagc atcctggtat cacagcttca gcatggactg acgttagagt
721 ttgaacacag tgattcacct cgtcgattgc gtcttgtgct tagtgaactg ttggcaatta
781 tgaacaaggt gtctgagtcc aacggagaat tttttttcaa gtcttctgaa ctttttgaga
841 gtccagtata tttggaggaa gctgcagatg tactttgtat tttacaagca gagctccctt
901 ccttgctccc tatagttgat gtagctgaag ctttgctaca tgttagaaat ggtgcctggt
961 tcttgtgtct cttggtggcc aatgttcctg atagttttaa tgaagtttgt aggggcctga
1021 taaaaaatgg agaacgacaa gatgaagaaa gtcttggagg aaggcgcagg acagatgcct
1081 tacgcttctt gtgtaaaatg aatccttctc aggccctcaa ggtccgaggc atggtggtgg
1141 aagaatgtca cttgccaggc cttggtgtgg ctttgacatt ggatcatact aaaaatgaag
1201 cttgtgagga tggagtgagt gacttggttt gttttgtaag tggtttgctt cttggaacaa
1261 atgcgaaagt ccggacttgg tttggaactt ttatccgaaa tggacagcag agaaaaagag
1321 agaccagcag ttctgtcctt tggcagatga gaaggcagct tcttctggag ttgatgggca
1381 ttcttcccac agtaagaagc acccgaattg tggaagaagc tgatgtggat atggagccca
1441 atgtgtctgt gtattcgggg ctgaaagaag agcatgttgt gaaagccagt gcactcttac
1501 gtctgtactg tgctttgatg gggatcgctg gactcaaacc aactgaagaa gaagctgagc
1561 aattactgca gttgatgacg agccgtcctc ctgctacgcc agctggggtt cgctttgttt
1621 cactttcctt ttgtatgcta caggcctttt ctacacttgt cagtacacct gaacaggagc
1681 agctgatggt ggtgtggcta agttggatga taaaagaaga agcgtatttt gagagtactt
1741 caggcgtctc tgcttctttt ggggagatgt tattattggt ggctatgtac tttcacagca
1801 accagcttag tgctatcatt gacttggtct gttccacttt ggggatgaag attgtaatta
1861 agccaagctc cttgagcagg atgaagacaa tcttcacaca ggaaattttt actgagcagg
1921 ttgtcacagc tcatgcagtt cgggtccctg tcaccagcaa cctgagtgcc aacattactg
1981 gatttttgcc tattcattgt atttaccagc ttctcaggag ccgttccttt accaagcaca
2041 aagtgtcaat aaaagattgg atttatagac agctgtgtga aacctctact ccacttcatc
2101 ctcaattact tcctttgatt gatgtgtaca taaattctat acttactcct gcgtcgaaat
2161 ctaatccaga agccacaaat cagccagtca cagaacagga gatactcaat attttccaag
2221 gagtcattgg gggtgacaac atccgcctta atcagcgttt cagtatcaca gcacagcttt
2281 tggtgctcta ctatatactg tcttatgaag aggctcttct agcaaacacg aagactttag
2341 ctgccatgca aagaaagccc aaatcatatt cttcttcttt aatggatcag attcctatca
2401 aattccttat tcgacaggct caagggctgc agcaggagtt gggagggttg cattcagctt
2461 tactacgtct ccttgctact aactacccac atttatgtat tgtggatgac tggatttgtg
2521 aagaagaaat cacagggact gatgccctgc tacggcgaat gctcctgact aataatgcta
2581 aaaatcattc tcccaaacaa ctccaagaag cattttcagc tgtcccagta aatcacacac
2641 aagtgatgca gattatagaa cacttgactc tactctctgc cagtgaactt ataccatatg
2701 cggaagtgtt aacatccaat atgagccagc tattgaattc aggggttcca cggagaattc
2761 tgcaaacagt caataaacta tggatggttc ttaatactgt gatgcctaga aggctatggg
2821 taatgacggt taatgcactt cagccttcaa taaagtttgt acgacaacaa aagtatactc
2881 agaatgacct gatgatagat cctctcattg tcctaaggtg tgatcagagg gttcacagat
2941 gccccccact gatggatatt accctacaca tgttgaatgg atatcttctc gcatctaaag
3001 cctaccttag tgctcatctg aaggaaacag agcaagatag gccttcccag aataatacaa
3061 ttggtttagt tggacaaact gatgctccgg aagttaccag ggaagaattg aaaaatgcat
3121 tactggccgc tcaggatagt gcagctgtcc agattctctt agagatttgc ctacctactg
3181 aagaggagaa agcaaatggt gtcaatccag atagcttgtt aagaaatgtt caaagtgtta
3241 ttaccaccag cgctccaaat aagggaatgg aggaaggaga agacaatttg ctctgtatcc
3301 ttcgagaagt tcagtgcctt atctgttgtc tcttgcacca aatgtacatt gcagatccca
3361 acattgctaa gcttgttcac tttcagggtt atccatgtga acttttgcct ctgacggtcg
3421 caggtattcc atctatgcac atctgtctag atttcatacc tgagcttatt gcacagccag
3481 aacttgagaa acagatattt gctatccagt tgctttctca cttgtgtata caatatgcat
3541 taccaaagtc acttagtgtg gctcgtttag ctgtcaatgt catgggaact ttgttaacag
3601 ttttaacaca ggctaagcgg tatgctcttt ttatgccaac tctgccaagt ttggtctctt
3661 tttgtcgagc atttcctcca ttgtatgagg atattatgtc tttgctgatc caaatagggc
3721 aagtttgtgc ctctgatgtt gccactcaga caagagacat tgatccaatt attacacgtc
3781 ttcaacaaat aaaggagaaa ccaagtggat ggtctcaaat ctgtaaagat tcatcttata
3841 aaaatggatc cagggacact ggaagcatgg atcctgatgt acagctctgt cactgtattg
3901 aaagaacagt aattgaaata ataaatatga gtgttagtgg aatttaaaac aaaatttaaa
3961 acaacaaaaa gttgtttgct gcatataccc aacatgaatc tgcatattag taacaactct
4021 aaactgaatg ggaacagtaa agtattgtct tggaatcact aaaacaattc aattcaacat
4081 gagtatagtt tagaacttta tgagaattat gcttgcttgt ttctgattgg cacatctttg
4141 gatctacttt gctgatatgt ttctattgta gcagctgagc tttttttttt tccactggga
4201 acacatgtaa gaaactcatt attggaaagg gaatttggcc ttgtatttag ctttt
//