LOCUS AK298561 2064 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ53290 complete cds, highly similar to
Cytoplasmic dynein 1 intermediate chain 2.
ACCESSION AK298561
VERSION AK298561.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2064)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2064
/cell_type="normal mesangial cells (NHMC56046-2)"
/clone="MESAN2012053"
/clone_lib="MESAN2"
/db_xref="H-InvDB:HIT000493185"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal mesangial cells"
/organism="Homo sapiens"
CDS 72..1964
/codon_start=1
/note="highly similar to Cytoplasmic dynein 1
intermediate chain 2"
/protein_id="BAG60755.1"
/transl_table=1
/translation="MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAV
APVQEESDLEKKRREAEALLQSMGLTPESPIAEQPLRVVTADTCLFHYLVPPPMSPSS
KSVSTPSEAGSQDSGDGAVGSRRGPIKLGMAKITQVDFPPREIVTYTKETQTPVMAQP
KEDEEEDDDAVAPKPPIEPEEEKTLKKDEENDSKAPPHELTEEEKQQILHSEEFLSFF
DHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSC
LDWSSQYPELLVASYNNNEDAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAK
FHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLIS
ISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYT
ACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKP
LYSFEDNADYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALN
RVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINANRADAEEEA
ATRIPA"
BASE COUNT 633 a 404 c 491 g 536 t
ORIGIN
1 acttaaggcc cctgggttga ctgggttctt tgttttatct accggcttct gctttacgac
61 aggtcacaaa catgtcagac aaaagtgaat taaaggctga gttggaacgt aagaagcagc
121 gactggccca aatcagagag gaaaagaaga gaaaagaaga agaaaggaaa aaaaaagaaa
181 cagaccagaa gaaggaagct gttgctcctg tgcaagaaga atcagatctt gaaaaaaaaa
241 ggagagaagc tgaagcattg cttcaaagca tggggctaac tccagaatcc cccattgctg
301 agcaacctct ccgtgtagta acagcggata cctgtctatt tcactattta gtccctcctc
361 ctatgtctcc atcctccaaa tctgtgagca ctccaagtga agctggaagc caagactctg
421 gagatggcgc cgtgggatct agacgaggac ctattaaact tggaatggct aaaatcacgc
481 aagtcgactt tcctcctcga gaaattgtca cgtatacaaa ggaaactcag actccagtta
541 tggctcaacc caaagaagat gaagaggaag atgatgatgc agtggctcct aaaccaccta
601 ttgaacctga agaagagaaa actttaaaga aagatgagga aaatgatagt aaagctcccc
661 ctcatgagct gactgaagaa gaaaagcaac aaatcttgca ctctgaggaa tttttaagtt
721 tctttgacca ttctacaaga attgtagaaa gagctctttc tgagcagatt aacatcttct
781 ttgactatag tgggagagat ttggaagaca aagaaggaga gattcaagca ggtgctaaac
841 tgtcattaaa tcgacaattt tttgacgaac gttggtcaaa gcatcgggtg gttagttgtt
901 tggattggtc atctcagtat ccggagttac tcgtggcttc ctataacaac aatgaagatg
961 cccctcatga gcctgatggt gtggcccttg tatggaatat gaaatacaaa aaaactaccc
1021 cagagtatgt gtttcactgc cagtcagctg tgatgtctgc cacatttgca aaatttcatc
1081 caaatcttgt tgttggtggt acatattcag gccaaattgt gctttgggat aaccgtagca
1141 ataaaagaac tccagtgcaa agaactccac tgtcagcagc tgcacacaca caccctgtat
1201 attgtgtaaa tgttgttgga acacaaaatg ctcacaatct gattagcatc tctactgatg
1261 gaaaaatttg ttcatggagt ctggacatgc tttcccatcc acaggatagc atggagttgg
1321 ttcataaaca gtcaaaagca gtagctgtga catctatgtc cttccctgtt ggagatgtca
1381 acaactttgt tgttgggagt gaagaaggtt ctgtgtacac agcatgccgc catggcagca
1441 aagctggaat cagtgagatg tttgaggggc atcaaggacc aatcactggc atccattgtc
1501 atgcagctgt tggagcagta gacttctcac atctttttgt cacttcatcg tttgactgga
1561 cagtaaagct ttggacaact aagaataaca agcctttgta ttcatttgaa gataatgcag
1621 actatgttta tgatgttatg tggtcaccta cccacccagc cctgtttgcc tgtgtggatg
1681 gcatggggag attggatttg tggaatctca ataatgacac agaggtacca actgccagca
1741 tttctgtgga gggtaatcct gctcttaatc gtgtgagatg gacccattct ggcagagaga
1801 ttgctgtggg tgattctgaa ggacagattg ttatatacga tgtgggagag cagattgctg
1861 ttccccgcaa tgatgaatgg gcacggtttg gccgaacact tgcagaaatt aatgcaaacc
1921 gagctgatgc agaggaggaa gcagctaccc gaatacctgc ttagttcctg aaaaggggag
1981 tgtaactagt ggatttggga aaggttctta agtagatcct gagactattt gcatgcttct
2041 gtctaaatga taattaaaag gaaa
//