LOCUS AK297118 2600 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ60298 complete cds, highly similar to Seprase
(EC 3.4.21.-).
ACCESSION AK297118
VERSION AK297118.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2600)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2600
/cell_type="normal dermal fibroblasts (Neonatal Skin)
(NHDF2564)"
/clone="DFNES2005461"
/clone_lib="DFNES2"
/db_xref="H-InvDB:HIT000491742"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, normal dermal fibroblasts"
/organism="Homo sapiens"
CDS 132..2339
/codon_start=1
/note="highly similar to Seprase (EC 3.4.21.-)"
/protein_id="BAG59624.1"
/transl_table=1
/translation="MKTWVKIVFGVATSAVLALLVMCIVLRPSRVHNSEENTMRALTL
KDILNGTFSYKTFFPNWISGQEYLHQSADNNIVLYNIETGQSYTILSNRTMLWRYSYT
ATYYIYDLSNGEFVRGNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITF
NGRENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTDIPVIAYSYYGDEQ
YPRTINIPYPKAGAKNPVVRIFIIDTTYPAYVGPQEVPVPAMIASSDYYFSWLTWVTD
ERVCLQWLKRVQNVSVLSICDFREDWQTWDCPKTQEHIEESRTGWAGGFFVSTPVFSY
DAISYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEE
YPGRRNIYRISIGSYPPSKKCVTCHLRKERCQYYTASFSDYAKYYALVCYGPGIPIST
LHDGRTDQEIKILEENKELENALKNIQLPKEEIKKLEVDEITLWYKMILPPQFDRSKK
YPLLIQVYGGPCSQSVRSVFAVNWISYLASKEGMVIALVDGRGTAFQGDKLLYAVYRK
LGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWSYGGYVSSLALASGTGLFKCGIAVAP
VSSWEYYASVYTERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHF
QNSAQIAKALVNAQVDFQAMWYSDQNHGLSGLSTNHLYTHMTHFLKQCFSLSD"
BASE COUNT 838 a 490 c 526 g 746 t
ORIGIN
1 aaaaaaaaac cttgtcctgg cttcagcttc caactacaaa gacagacttg gtccttttca
61 acggttttca cagatccagt gacccacgct ctgaagacag aattagctaa ctttcaaaaa
121 catctggaaa aatgaagact tgggtaaaaa tcgtatttgg agttgccacc tctgctgtgc
181 ttgccttatt ggtgatgtgc attgtcttac gcccttcaag agttcataac tctgaagaaa
241 atacaatgag agcactcaca ctgaaggata ttttaaatgg aacattttct tataaaacat
301 tttttccaaa ctggatttca ggacaagaat atcttcatca atctgcagat aacaatatag
361 tactttataa tattgaaaca ggacaatcat ataccatttt gagtaataga accatgcttt
421 ggagatactc ttacacagca acatattaca tctatgacct tagcaatgga gaatttgtaa
481 gaggaaatga gcttcctcgt ccaattcagt atttatgctg gtcgcctgtt gggagtaaat
541 tagcatatgt ctatcaaaac aatatctatt tgaaacaaag accaggagat ccaccttttc
601 aaataacatt taatggaaga gaaaataaaa tatttaatgg aatcccagac tgggtttatg
661 aagaggaaat gcttgctaca aaatatgctc tctggtggtc tcctaatgga aaatttttgg
721 catatgcgga atttaatgat acggatatac cagttattgc ctattcctat tatggcgatg
781 aacaatatcc tagaacaata aatattccat acccaaaggc tggagctaag aatcccgttg
841 ttcggatatt tattatcgat accacttacc ctgcgtatgt aggtccccag gaagtgcctg
901 ttccagcaat gatagcctca agtgattatt atttcagttg gctcacgtgg gttactgatg
961 aacgagtatg tttgcagtgg ctaaaaagag tccagaatgt ttcggtcctg tctatatgtg
1021 acttcaggga agactggcag acatgggatt gtccaaagac ccaggagcat atagaagaaa
1081 gcagaactgg atgggctggt ggattctttg tttcaacacc agttttcagc tatgatgcca
1141 tttcgtacta caaaatattt agtgacaagg atggctacaa acatattcac tatatcaaag
1201 acactgtgga aaatgctatt caaattacaa gtggcaagtg ggaggccata aatatattca
1261 gagtaacaca ggattcactg ttttattcta gcaatgaatt tgaagaatac cctggaagaa
1321 gaaacatcta cagaattagc attggaagct atcctccaag caagaagtgt gttacttgcc
1381 atctaaggaa agaaaggtgc caatattaca cagcaagttt cagcgactac gccaagtact
1441 atgcacttgt ctgctacggc ccaggcatcc ccatttccac ccttcatgat ggacgcactg
1501 atcaagaaat taaaatcctg gaagaaaaca aggaattgga aaatgctttg aaaaatatcc
1561 agctgcctaa agaggaaatt aagaaacttg aagtagatga aattacttta tggtacaaga
1621 tgattcttcc tcctcaattt gacagatcaa agaagtatcc cttgctaatt caagtgtatg
1681 gtggtccctg cagtcagagt gtaaggtctg tatttgctgt taattggata tcttatcttg
1741 caagtaagga agggatggtc attgccttgg tggatggtcg aggaacagct ttccaaggtg
1801 acaaactcct ctatgcagtg tatcgaaagc tgggtgttta tgaagttgaa gaccagatta
1861 cagctgtcag aaaattcata gaaatgggtt tcattgatga aaaaagaata gccatatggg
1921 gctggtccta tggaggatac gtttcatcac tggcccttgc atctggaact ggtcttttca
1981 aatgtggtat agcagtggct ccagtctcca gctgggaata ttacgcgtct gtctacacag
2041 agagattcat gggtctccca acaaaggatg ataatcttga gcactataag aattcgactg
2101 tgatggcaag agcagaatat ttcagaaatg tagactatct tctcatccac ggaacagcag
2161 atgataatgt gcactttcaa aactcagcac agattgctaa agctctggtt aatgcacaag
2221 tggatttcca ggcaatgtgg tactctgacc agaaccacgg cttatccggc ctgtccacga
2281 accacttata cacccacatg acccacttcc taaagcagtg tttctctttg tcagactaaa
2341 aacgatgcag atgcaagcct gtatcagaat ctgaaaacct tatataaacc cctcagacag
2401 tttgcttatt ttatttttta tgttgtaaaa tgctagtata aacaaacaaa ttaatgttgt
2461 tctaaaggct gttaaaaaaa agatgaggac tcagaagttc aagctaaata ttgtttacat
2521 tttctggtac tctgtgaaag aagagaaaag ggagtcatgc attttgcttt ggacacagtg
2581 ttttatcacc tgttcatttg
//