LOCUS AK295874 3737 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58533 complete cds, highly similar to
Leucyl-tRNA synthetase, cytoplasmic (EC 6.1.1.4).
ACCESSION AK295874
VERSION AK295874.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3737)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3737
/clone="BRHIP3040648"
/clone_lib="BRHIP3"
/db_xref="H-InvDB:HIT000490498"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="hippocampus"
CDS 174..3542
/codon_start=1
/note="highly similar to Leucyl-tRNA synthetase,
cytoplasmic (EC 6.1.1.4)"
/protein_id="BAG58672.1"
/transl_table=1
/translation="MNGRLHLGHTFSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIK
ACADKLKREIELYGCPPDFPDEEEEEEETSVKTEDIIIKDKAKGKKSKAAAKAGSSKY
QWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYY
DSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVL
EPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKA
ARNMSYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGV
VTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFGNLSAVTIC
DELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQKKMIDAGDA
LIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRNFE
ATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAE
SPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLV
PNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSA
DGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRSGPASTFND
RVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMHRELVFRFI
EVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLR
LRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPD
NKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVY
LTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTK
IEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPIS
EHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH"
BASE COUNT 1117 a 701 c 885 g 1034 t
ORIGIN
1 aggtgtgaag cgtgtgcttt agtttcgtgg gaggcctggc atccccgaga gggaggggaa
61 aggtaaccac tcctttgtgg aggtcgccag ggtcattgtc gtggatttgc acagtcggct
121 gggcggtgca atggcgcaag ggcaagtatt ttgtaacctt cccatatcca tatatgaatg
181 gacgccttca tttgggacac acgttttctt tatccaaatg tgagtttgct gtagggtacc
241 agcgattgaa aggaaaatgt tgtctgtttc cctttggcct gcactgtact ggaatgccta
301 ttaaggcatg tgctgataag ttgaaaagag aaatagagct gtatggttgc ccccctgatt
361 ttccagatga agaagaggaa gaggaagaaa ccagtgttaa aacagaagat ataataatta
421 aggataaagc taaaggaaaa aagagtaaag ctgctgctaa agctggatct tctaaatacc
481 agtggggcat tatgaaatcc cttggcctgt ctgatgaaga gatagtaaaa ttttctgaag
541 cagaacattg gcttgattat ttcccgccac tggctattca ggatttaaaa agaatgggtt
601 tgaaggtaga ctggcgtcgt tccttcatca ccactgatgt taatccttac tatgattcat
661 ttgtcagatg gcaattttta acattaagag aaagaaacaa aattaaattt gggaagcggt
721 atacaattta ctctccgaaa gatggacagc cttgcatgga tcatgataga caaactggag
781 agggtgttgg acctcaggaa tatactttac tcaaattgaa ggtgcttgag ccatacccat
841 ctaaattaag tggcctgaaa ggtaaaaata ttttcttggt ggctgctact ctcagacctg
901 agaccatgtt tgggcagaca aattgttggg ttcgtcctga tatgaagtac attggatttg
961 agacggtgaa tggtgatata ttcatctgta cccaaaaagc agccaggaat atgtcatacc
1021 agggctttac caaagacaat ggcgtggtgc ctgttgttaa ggaattaatg ggggaggaaa
1081 ttcttggtgc atcactttct gcacctttaa catcatacaa ggtgatctat gttctcccaa
1141 tgctaactat taaggaggat aaaggcactg gtgtggttac aagtgttcct tccgactccc
1201 ctgatgatat tgctgccctc agagacttga agaaaaagca agccttacga gcaaaatatg
1261 gaattagaga tgacatggtc ttgccatttg agccggtgcc agtcattgaa atcccaggtt
1321 ttggaaatct ttctgctgta accatttgtg atgagttgaa aattcagagc cagaatgacc
1381 gggaaaaact tgcagaagca aaggagaaga tatatctaaa aggattttat gagggtatca
1441 tgttggtgga tggatttaaa ggacagaagg ttcaagatgt aaagaagact attcagaaaa
1501 agatgattga cgctggagat gcacttattt acatggaacc agagaaacaa gtgatgtcca
1561 ggtcgtcaga tgaatgtgtt gtggctctgt gtgaccagtg gtacttggat tatggagaag
1621 agaattggaa gaaacagaca tctcagtgct tgaagaacct ggaaacattc tgtgaggaga
1681 ccaggaggaa ttttgaagcc accttaggtt ggctacaaga acatgcttgc tcaagaactt
1741 atggtctagg cactcacctg ccttgggatg agcagtggct gattgaatca ctttctgact
1801 ccactattta catggcattt tacacagttg cacacctatt gcaggggggt aacttgcatg
1861 gacaggcaga gtctccgctg ggcattagac cgcaacagat gaccaaggaa gtttgggatt
1921 atgttttctt caaggaggct ccatttccta agactcagat tgcaaaggaa aaattagatc
1981 agttaaagca ggagtttgaa ttctggtatc ctgttgatct tcgcgtctct ggcaaggatc
2041 ttgttccaaa tcatctttca tattaccttt ataatcatgt ggctatgtgg ccggaacaaa
2101 gtgacaaatg gcctacagct gtgagagcaa atggacatct cctcctgaac tctgagaaga
2161 tgtcaaaatc cacaggcaac ttcctcactt tgacccaagc tattgacaaa ttttcagcag
2221 atggaatgcg tttggctttg gctgatgctg gtgacactgt agaagatgcc aactttgtgg
2281 aagccatggc agatgcaggt attctccgtc tgtacacctg ggtagagtgg gtgaaagaaa
2341 tggttgccaa ctgggacagc ctaagaagtg gtcctgccag cactttcaat gatagagttt
2401 ttgccagtga attgaatgca ggaattataa aaacagatca aaactatgaa aagatgatgt
2461 ttaaagaagc tttgaaaaca gggttttttg agtttcaggc cgcaaaagat aagtaccgtg
2521 aattggctgt ggaagggatg cacagagaac ttgtgttccg gtttattgaa gttcagacac
2581 ttctcctcgc tccattctgt ccacatttgt gtgagcacat ctggacactc ctgggaaagc
2641 ctgactcaat tatgaatgct tcatggcctg tggcaggtcc tgttaatgaa gttttaatac
2701 actcctcaca gtatcttatg gaagtaacac atgaccttag actacgactc aagaactata
2761 tgatgccagc taaagggaag aagactgaca aacaacccct gcagaagccc tcacattgca
2821 ccatctatgt ggcaaagaac tatccacctt ggcaacatac caccctgtct gttctacgta
2881 aacactttga ggccaataac ggaaaactgc ctgacaacaa agtcattgct agtgaactag
2941 gcagtatgcc agaactgaag aaatacatga agaaagtcat gccatttgtt gccatgatta
3001 aggaaaatct ggagaagatg gggcctcgta ttctggattt gcaattagaa tttgatgaaa
3061 aggctgtgct tatggagaat atagtctatc tgactaattc gcttgagcta gaacacatag
3121 aagtcaagtt tgcctccgaa gcagaagata aaatcaggga agactgctgt cctgggaaac
3181 cacttaatgt ttttagaata gaacctggtg tgtccgtttc tctggtgaat ccccagccat
3241 ccaatggcca cttctcaacc aaaattgaaa tcaggcaagg agataactgt gattccataa
3301 tcaggcgttt aatgaaaatg aatcgaggaa ttaaagacct ttccaaagtg aaactgatga
3361 gatttgatga tccactgttg gggcctcgac gagttcctgt cctgggaaag gagtacaccg
3421 agaagacccc catttctgag catgctgttt tcaatgtgga cctcatgagc aagaaaattc
3481 atctgactga gaatgggata agggtggata ttggcgatac aataatctat ctggttcatt
3541 aaactcatgc acattggaga tttatcctgg tttcttagga atactactac tctgattgtg
3601 tctactgatt ggctatcaga accttaggct ggacctaaat agattgattt catttctaac
3661 catccaattc tgcatgtatt cataattcta tcaagtcatc tttgattcct ggacctaata
3721 aatttttttt ccctttc
//