LOCUS AK295002 2140 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ50959 complete cds, highly similar to Rap
guanine nucleotide exchange factor 4.
ACCESSION AK295002
VERSION AK295002.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2140)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2140
/clone="BRAWH3031805"
/clone_lib="BRAWH3"
/db_xref="H-InvDB:HIT000489626"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 212..1522
/codon_start=1
/note="highly similar to Rap guanine nucleotide exchange
factor 4"
/protein_id="BAH11946.1"
/transl_table=1
/translation="MAGLLAPPYGVMETGSNNDRIPDKENVPSEKILRAGKILRNAIL
SRAPHMIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNH
VDQEHHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMI
LRKPPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGE
EGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLR
VDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTP
EKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQGTE
QEKMDYALNSKRRVIRLVLQWAAMYGDLLQEDDVSMYLYQMMPG"
BASE COUNT 628 a 469 c 534 g 509 t
ORIGIN
1 attagtgcta atatgctgca gaaaagccaa gataaatcag tccagctctc atacatcact
61 aagggagaag ctgattgcaa cttgaccatg tgcctgagta gtcccatcag tgagaagagg
121 agaacatgga tttaaaaaaa aactagaatg tacccctagg gctcctgccg tggtggacct
181 gggagaagtg tctggtaaat atcgacagta tatggcagga cttctggctc ctccttatgg
241 tgttatggaa acgggctcta acaatgacag gattcctgac aaggagaacg tcccttcaga
301 gaagatcctc agagctggaa aaattttacg aaatgccatt ctctctcgag cacctcacat
361 gataagagat agaaaatacc acctaaagac atacagacaa tgctgtgtgg gaactgaact
421 ggtggactgg atgatgcagc agacaccatg tgttcactcc cggactcaag ctgttggcat
481 gtggcaagtc ctgttagaag atggtgttct caaccacgtg gaccaggagc accatttcca
541 agacaaatat ttattctatc gatttctgga tgatgagcac gaggatgccc ctttgcctac
601 tgaggaggag aagaaggagt gtgatgagga gctccaggac accatgctgc tgctgtcaca
661 gatgggcccc gacgcccaca tgaggatgat ccttcgcaaa ccacctggcc agaggactgt
721 ggatgaccta gagattatct atgaggagct tcttcatatt aaagccttat cccatctttc
781 taccacagtg aaacgagagt tagcaggtgt tctcattttt gagtctcacg ccaaaggagg
841 gactgtgttg tttaaccagg gggaagaagg tacctcctgg tacattattc taaaaggatc
901 agtgaatgta gtcatttacg gcaagggtgt ggtctgcacc ctgcatgaag gagatgactt
961 cggcaagtta gcactagtga atgatgcccc acgagctgcc tctatcgtct tacgagaaga
1021 taactgccat ttcttaagag tagacaagga ggatttcaac cggatcctaa gggacgtgga
1081 ggcgaataca gtcagactta aagaacatga ccaagatgtc ttggtgctgg agaaggtccc
1141 agcagggaac agagcttcta atcaaggaaa ctcacagcct cagcaaaagt atactgtgat
1201 gtcaggaaca cctgaaaaaa ttttagagca ttttctagaa acaatacgcc ttgaggcaac
1261 tttaaatgaa gcaacagatt ctgttttaaa tgactttatt atgatgcact gtgtttttat
1321 gccaaatacc cagctttgcc cggcactggt ggcccactac cacgcacagc cttcacaagg
1381 tacagaacag gagaaaatgg attatgccct caacagtaag aggcgagtca tccgcctggt
1441 tctacagtgg gctgccatgt atggagacct cctgcaagag gatgacgtgt ctatgtatct
1501 gtatcagatg atgcccggat gattgctgcc ctcaaggagc aactgccaga gttggagaag
1561 attgtcaagc aaatctcaga agatgcaaag gcaccacaaa agaagcacaa ggttcttttg
1621 caacagttca atacgggcga tgagagagcc cagaagcgcc agcctatccg cggctctgat
1681 gaagttctgt ttaaggtcta ttgcatggac cacacctaca caaccattcg ggtgccagtg
1741 gccacttcgg tgaaggaagt catcagtgca gttgccgaca agctgggctc cggggagggc
1801 ctgatcatag tcaagatgag ttccggagga gaaaaggtgg tgctcaaacc taatgatgtt
1861 tcagtattta cgacgctcac cattaatgga cgcctgtttg cttgcccgcg agagcaattc
1921 gattcactga ctcccttacc agaacaggaa ggcccaactg ttggaacagt gggaactttt
1981 gaactgatga gctccaaaga tttagcatac cagatgacaa tttatgattg ggaactcttc
2041 aactgcgtgc atgagctgga gctaatctat cacacatttg gaaggcataa ttttaaaaag
2101 accacagcaa acttggattt gttcctgagg agatttaatg
//