LOCUS AK294445 2961 bp mRNA linear HUM 21-JAN-2009
DEFINITION Homo sapiens cDNA FLJ57298 complete cds, highly similar to Rap
guanine nucleotide exchange factor 4.
ACCESSION AK294445
VERSION AK294445.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2961)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..2961
/clone="BRAMY3006504"
/clone_lib="BRAMY3"
/db_xref="H-InvDB:HIT000489069"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="amygdala"
CDS 126..2702
/codon_start=1
/note="highly similar to Rap guanine nucleotide exchange
factor 4"
/protein_id="BAH11769.1"
/transl_table=1
/translation="MAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPANTITK
VPSEKILRAGKILRNAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSR
TQAVGMWQVLLEDGVLNHVDQEHHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQ
DTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGV
LIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVND
APRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRAS
NQGNSQPQQKYTVMSGTPEKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQ
LCPALVAHYHAQPSQGTEQEKMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMAFLE
EFYVSVSDDARMIAALKEQLPELEKIVKQISEDAKAPQKKHKVLLQQFNTGDERAQKR
QPIRGSDEVLFKVYCMDHTYTTIRVPVATSVKEVISAVADKLGSGEGLIIVKMSSGGE
KVVLKPNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFELMSSKDLA
YQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCS
QLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFY
AEFESLMDPSRNHRAYRLTVAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKM
RMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPR
RP"
BASE COUNT 875 a 668 c 675 g 743 t
ORIGIN
1 agatagatat tttgtaagat tctagtcaat gtcagtattt aaactcctgg accatatgtg
61 gcttctctta gcagattttg caaaggaaac ttactccagt atgcaagact aaatatcgac
121 agtatatggc aggacttctg gctcctcctt atggtgttat ggaaacgggc tctaacaatg
181 acaggattcc tgacaaggag aacacacctc tcattgaacc tcacgttcct cttcgtcctg
241 ctaacaccat taccaaggtc ccttcagaga agatcctcag agctggaaaa attttacgaa
301 atgccattct ctctcgagca cctcacatga taagagatag aaaataccac ctaaagacat
361 acagacaatg ctgtgtggga actgaactgg tggactggat gatgcagcag acaccatgtg
421 ttcactcccg gactcaagct gttggcatgt ggcaagtcct gttagaagat ggtgttctca
481 accacgtgga ccaggagcac catttccaag acaaatattt attctatcga tttctggatg
541 atgagcacga ggatgcccct ttgcctactg aggaggagaa gaaggagtgt gatgaggagc
601 tccaggacac catgctgctg ctgtcacaga tgggccccga cgcccacatg aggatgatcc
661 ttcgcaaacc acctggccag aggactgtgg atgacctaga gattatctat gaggagcttc
721 ttcatattaa agccttatcc catctttcta ccacagtgaa acgagagtta gcaggtgttc
781 tcatttttga gtctcacgcc aaaggaggga ctgtgttgtt taaccagggg gaagaaggta
841 cctcctggta cattattcta aaaggatcag tgaatgtagt catttacggc aagggtgtgg
901 tctgcaccct gcatgaagga gatgacttcg gcaagttagc actagtgaat gatgccccac
961 gagctgcctc tatcgtctta cgagaagata actgccattt cttaagagta gacaaggagg
1021 atttcaaccg gatcctaagg gacgtggagg cgaatacagt cagacttaaa gaacatgacc
1081 aagatgtctt ggtgctggag aaggtcccag cagggaacag agcttctaat caaggaaact
1141 cacagcctca gcaaaagtat actgtgatgt caggaacacc tgaaaaaatt ttagagcatt
1201 ttctagaaac aatacgcctt gaggcaactt taaatgaagc aacagattct gttttaaatg
1261 actttattat gatgcactgt gtttttatgc caaataccca gctttgcccg gcactggtgg
1321 cccactacca cgcacagcct tcacaaggta cagaacagga gaaaatggat tatgccctca
1381 acaataagag gcgagtcatc cgcctggttc tacagtgggc tgccatgtat ggagacctcc
1441 tgcaagagga tgacgtgtct atggccttcc tggaggagtt ttatgtatct gtatcagatg
1501 atgcccggat gattgctgcc ctcaaggagc aactgccaga gttggagaag attgtcaagc
1561 aaatctcaga agatgcaaag gcaccacaaa agaagcacaa ggttcttttg caacagttca
1621 atacgggcga tgagagagcc cagaagcgcc agcctatccg cggctctgat gaagttctgt
1681 ttaaggtcta ttgcatggac cacacctaca caaccattcg ggtgccagtg gccacttcgg
1741 tgaaggaagt catcagtgca gttgccgaca agctgggctc cggggagggc ctgatcatag
1801 tcaagatgag ttccggagga gaaaaggtgg tgctcaaacc taatgatgtt tcagtattta
1861 cgacgctcac cattaatgga cgcctgtttg cttgcccgcg agagcaattc gattcactga
1921 ctcccttacc agaacaggaa ggcccaactg ttggaacagt gggaactttt gaactgatga
1981 gctccaaaga tttagcatac cagatgacaa tttatgattg ggaactcttc aactgcgtgc
2041 atgagctgga gctaatctat cacacatttg gaaggcataa ttttaaaaag accacagcaa
2101 acttggattt gttcctgagg agatttaatg aaattcagtt ttgggtcgtc actgagatct
2161 gcctttgttc tcagctcagc aagcgtgttc agctattaaa aaaatttatt aagatagcag
2221 cccactgtaa ggagtataaa aatctgaatt ccttttttgc catcgtcatg ggactaagta
2281 acgttgctgt gagccgcttg gcactaacgt gggagaaact gccaagcaag ttcaagaagt
2341 tctatgcgga gtttgaaagt ttaatggacc cttcaaggaa ccacagagcc tacaggctga
2401 cagtagctaa gctggaacct cctctcatcc ccttcatgcc tttgctcatt aaagatatga
2461 catttactca tgaggggaac aagacgttca ttgacaatct agtaaacttt gaaaaaatgc
2521 gcatgattgc aaatacggcc agaacagtga gatactacag gagccaaccc ttcaatcctg
2581 atgcagctca agctaataag aaccatcagg atgtccggag ttatgtacgg caattaaatg
2641 tgattgacaa ccagagaact ttatcacaga tgtcacacag attagagcct cgtcgaccat
2701 agacatttca aatgcccaaa gcaacagttt gtctccagtc cacaatcttt caaaaatgcc
2761 atttatgcta ctactgactg tattgccact agagaattct acaaaacaag caaaaacaca
2821 tcctgagaca cctcagggct gcattcagct taccagctac ctagcaagag aaggaattat
2881 ttgacatgcc taaagcttac acactctagc ttaggaatcc ccagtttgtg gctaccctgt
2941 ttcatttcca cttgtgccat c
//