LOCUS AK293750 3769 bp mRNA linear HUM 24-JUL-2008
DEFINITION Homo sapiens cDNA FLJ58157 complete cds, highly similar to
Leucyl-tRNA synthetase, cytoplasmic (EC 6.1.1.4).
ACCESSION AK293750
VERSION AK293750.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3769)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project focused on splicing variants
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..3769
/clone="BRACE3004379"
/clone_lib="BRACE3"
/db_xref="H-InvDB:HIT000488374"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="cerebellum"
CDS 125..3574
/codon_start=1
/note="highly similar to Leucyl-tRNA synthetase,
cytoplasmic (EC 6.1.1.4)"
/protein_id="BAG57172.1"
/transl_table=1
/translation="MAERKGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKG
KYFVTFPYPYMNGRLHLGHTFSLSKCEACADKLKREIELYGCPPDFPDEEEEEEETSV
KTEDIIIKDKAKGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPL
AIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDG
QPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQT
NCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILGAS
LSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIR
DDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIM
LVDGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYG
EENWKKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIES
LSDSTIYMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIA
KEKLDQLKQEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGH
LLLNSEKMSKSTGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRL
YTWVEWVKEMVANWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGF
FEFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNA
SWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVA
KNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKEN
LEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKP
LNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKL
MRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIY
LVH"
BASE COUNT 1148 a 701 c 893 g 1027 t
ORIGIN
1 gaagcgtgtg ctttagtttc gtgggaggcc tggcatcccc gagggggagg ggaaaggtaa
61 ccactccttt gtggaggtcg ccagggtcat tgtcgtggat ttgcacagtc ggctgggcgg
121 tgcaatggcg gaaagaaaag gaacagccaa agtggacttt ttgaagaaga ttgagaaaga
181 aatccaacag aaatgggata ctgagagagt gtttgaggtc aatgcatcta atttagagaa
241 acagaccagc aagggcaagt attttgtaac cttcccatat ccatatatga atggacgcct
301 tcatttggga cacacgtttt ctttatccaa atgtgaggca tgtgctgata agttgaaaag
361 agaaatagag ctgtatggtt gcccccctga ttttccagat gaagaagagg aagaggaaga
421 aaccagtgtt aaaacagaag atataataat taaggataaa gctaaaggaa aaaagagtaa
481 agctgctgct aaagctggat cttctaaata ccagtggggc attatgaaat cccttggcct
541 gtctgatgaa gagatagtaa aattttctga agcagaacat tggcttgatt atttcccgcc
601 actggctatt caggatttaa aaagaatggg tttgaaggta gactggcgtc gttccttcat
661 caccactgat gttaatcctt actatgattc atttgtcaga tggcaatttt taacattaag
721 agaaagaaac aaaattaaat ttgggaagcg gtatacaatt tactctccga aagatggaca
781 gccttgcatg gatcatgata gacaaactgg agagggtgtt ggacctcagg aatatacttt
841 actcaaattg aaggtgcttg agccataccc atctaaatta agtggcctga aaggtaaaaa
901 tattttcttg gtggctgcta ctctcagacc tgagaccatg tttgggcaga caaattgttg
961 ggttcgtcct gatatgaagt acattggatt tgagacggtg aatggtgata tattcatctg
1021 tacccaaaaa gcagccagga atatgtcata ccagggcttt accaaagaca atggcgtggt
1081 gcctgttgtt aaggaattaa tgggggagga aattcttggt gcatcacttt ctgcaccttt
1141 aacatcatac aaggtgatct atgttctccc aatgctaact attaaggagg ataaaggcac
1201 tggtgtggtt acaagtgttc cttccgactc ccctgatgat attgctgccc tcagagactt
1261 gaagaaaaag caagccttac gagcaaaata tggaattaga gatgacatgg tcttgccatt
1321 tgagccggtg ccagtcattg aaatcccagg ttttggaaat ctttctgctg taaccatttg
1381 tgatgagttg aaaattcaga gccagaatga ccgggaaaaa cttgcagaag caaaggagaa
1441 gatatatcta aaaggatttt atgagggtat catgttggtg gatggattta aaggacagaa
1501 ggttcaagat gtaaagaaga ctattcagaa aaagatgatt gacgctggag atgcacttat
1561 ttacatggaa ccagagaaac aagtgatgtc caggtcgtca gatgaatgtg ttgtggctct
1621 gtgtgaccag tggtacttgg attatggaga agagaattgg aagaaacaga catctcagtg
1681 cttgaagaac ctggaaacat tctgtgagga gaccaggagg aattttgaag ccaccttagg
1741 ttggctacaa gaacatgctt gctcaagaac ttatggtcta ggcactcacc tgccttggga
1801 tgagcagtgg ctgattgaat cactttctga ctccactatt tacatggcat tttacacagt
1861 tgcacaccta ttgcaggggg gtaacttgca tggacaggca gagtctccgc tgggcattag
1921 accgcaacag atgaccaagg aagtttggga ttatgttttc ttcaaggagg ctccatttcc
1981 taagactcag attgcaaagg aaaaattaga tcagttaaag caggagtttg aattctggta
2041 tcctgttgat cttcgcgtct ctggcaagga tcttgttcca aatcatcttt catattacct
2101 ttataatcat gtggctatgt ggccggaaca aagtgacaaa tggcctacag ctgtgagagc
2161 aaatggacat ctcctcctga actctgagaa gatgtcaaaa tccacaggca acttcctcac
2221 tttgacccaa gctattgaca aattttcagc agatggaatg cgtttggctc tggctgatgc
2281 tggtgacact gtagaagatg ccaactttgt ggaagccatg gcagatgcag gtattctccg
2341 tctgtacacc tgggtagagt gggtgaaaga aatggttgcc aactgggaca gcctaagaag
2401 tggtcctgcc agcactttca atgatagagt ttttgccagt gaattgaatg caggaattat
2461 aaaaacagat caaaactatg aaaagatgat gtttaaagaa gctttgaaaa cagggttttt
2521 tgagtttcag gccgcaaaag ataagtaccg tgaattggct gtggaaggga tgcacagaga
2581 acttgtgttc cggtttattg aagttcagac acttctcctc gctccattct gtccacattt
2641 gtgtgagcac atctggacac tcctgggaaa gcctgactca attatgaatg cttcatggcc
2701 tgtggcaggt cctgttaatg aagttttaat acactcctca cagtatctta tggaagtaac
2761 acatgacctt agactacgac tcaagaacta tatgatgcca gctaaaggga agaagactga
2821 caaacaaccc ctgcagaagc cctcacattg caccatctat gtggcaaaga actatccacc
2881 ttggcaacat accaccctgt ctgttctacg taaacacttt gaggccaata acggaaaact
2941 gcctgacaac aaagtcattg ctagtgaact aggcagtatg ccagaactga agaaatacat
3001 gaagaaagtc atgccatttg ttgccatgat taaggaaaat ctggagaaga tggggcctcg
3061 tattctggat ttgcaattag aatttgatga aaaggctgtg cttatggaga atatagtcta
3121 tctgactaat tcgcttgagc tagaacacat agaagtcaag tttgcctccg aagcagaaga
3181 taaaatcagg gaagactgct gtcctgggaa accacttaat gtttttagaa tagaacctgg
3241 tgtgtccgtt tctctggtga atccccagcc atccaatggc cacttctcaa ccaaaattga
3301 aatcaggcaa ggagataact gtgattccat aatcaggcgt ttaatgaaaa tgaatcgagg
3361 aattaaagac ctttccaaag tgaaactgat gagatttgat gatccactgt tggggcctcg
3421 acgagttcct gtcctgggaa aggagtacac cgagaagacc cccatttctg agcatgctgt
3481 tttcaatgtg gacctcatga gcaagaaaat tcatctgact gagaatggga taagggtgga
3541 tattggcgat acaataatct atctggttca ttaaactcat gcacattgga gatttatcct
3601 ggtttcttag gaatactact actctgattg tgtctactga ttggctatca gaaccttagg
3661 ctggacctaa atagattgat ttcatttcta accatccaat tctgcatgta ttcataattc
3721 tatcaagtca tctttgattc ctggacctaa taaatttttt ttccctttc
//