LOCUS AK291825 1893 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ77428 complete cds, highly similar to Homo
sapiens sterile alpha motif and leucine zipper containing kinase
AZK (ZAK), transcript variant 2, mRNA.
ACCESSION AK291825
VERSION AK291825.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1893)
AUTHORS Isogai,T. and Yamamoto,J.
TITLE Direct Submission
JOURNAL Submitted (09-OCT-2007) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Reverse Proteomics Research Institute; 1-9-11 Kaji-cho,
Chiyoda-ku, Tokyo 101-0044, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Wakamatsu,A., Yamamoto,J., Kimura,K., Ishii,S., Watanabe,K.,
Sugiyama,A., Murakawa,K., Kaida,T., Tsuchiya,K., Fukuzumi,Y.,
Kumagai,A., Oishi,Y., Yamamoto,S., Ono,Y., Komori,Y., Yamazaki,M.,
Kisu,Y., Nishikawa,T., Sugano,S., Nomura,N. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2007)
COMMENT Human cDNA sequencing project focused on splicing variants of mRNA
in NEDO functional analysis of protein and research application
project supported by Ministry of Economy, Trade and Industry,
Japan; cDNA selection for complete cds sequencing: Reverse
Proteomics Research Institute (REPRORI), Hitachi, Ltd., Japan
(Hitachi) and Japan Biological Informatics Consortium, Japan
(JBIC); cDNA complete cds sequencing: JBIC; cDNA library
construction: Helix Research Institute supported by Japan Key
Technology Center, Japan (HRI); cDNA 5'- & 3'-end sequencing:
Research Association for Biotechnology, Japan, Biotechnology
Center, National Institute of Technology and Evaluation, Japan and
HRI; cDNA mapping to human genome: Central Research Laboratory,
Hitachi; evaluation and annotation: REPRORI.
FEATURES Location/Qualifiers
source 1..1893
/cell_type="pulmonary artery endothelial cells (HPAEC)"
/clone="PUAEN2000438"
/clone_lib="PUAEN2"
/db_xref="H-InvDB:HIT000424622"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="primary culture, pulmonary artery endothelial
cells"
/organism="Homo sapiens"
CDS 93..1460
/codon_start=1
/note="highly similar to Homo sapiens sterile alpha motif
and leucine zipper containing kinase AZK (ZAK),
transcript variant 2, mRNA"
/protein_id="BAF84514.1"
/transl_table=1
/translation="MSSHGASFVQIKFDDLQFFEYCGGGSFGSVYRAKWISQDKEVAV
KKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEM
DMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNH
TTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLV
VEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSF
LHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLLPL
AARMSEESYFESKTEESNSAEMSCQITATSNGEGHGMNPSLQAMMLMGFGDIFSMNKA
GAVMHSGMQINMQAKQNSSKTTSKRRGKKVNMALGFSDFDLSEGDDDDDDDGEEEDND
MDNSE"
BASE COUNT 580 a 358 c 441 g 514 t
ORIGIN
1 ctgggcgcgc ggggcctccg cgcccccggc tgctgctcac gccccgcccg ggagccagat
61 tttgtggaag tataatactt tgtcattatg agatgtcgtc tcacggtgcc tcctttgtgc
121 aaattaaatt tgatgacttg cagttttttg aatactgcgg tggaggaagt tttgggagtg
181 tttatcgagc caaatggata tcacaggaca aggaggtggc tgtaaagaag ctcctcaaaa
241 tagagaaaga ggcagaaata ctcagtgtcc tcagtcacag aaacatcatc cagttttatg
301 gagtaattct tgaacctccc aactatggca ttgtcacaga atatgcttct ctgggatcac
361 tctatgatta cattaacagt aacagaagtg aggagatgga tatggatcac attatgacct
421 gggccactga tgtagccaaa ggaatgcatt atttacatat ggaggctcct gtcaaggtga
481 ttcacagaga cctcaagtca agaaacgttg ttatagctgc tgatggagta ttgaagatct
541 gtgactttgg tgcctctcgg ttccataacc atacaacaca catgtccttg gttggaactt
601 tcccatggat ggctccagaa gttatccaga gtctccctgt gtcagaaact tgtgacacat
661 attcctatgg tgtggttctc tgggagatgc taacaaggga ggtccccttt aaaggtttgg
721 aaggattaca agtagcttgg cttgtagtgg aaaaaaacga gagattaacc attccaagca
781 gttgccccag aagttttgct gaactgttac atcagtgttg ggaagctgat gccaagaaac
841 ggccatcatt caagcaaatc atttcaatcc tggagtccat gtcaaatgac acgagccttc
901 ctgacaagtg taactcattc ctacacaaca aggcggagtg gaggtgcgaa attgaggcaa
961 ctcttgagag gctaaagaaa ctagagcgtg atctcagctt taaggagcag gagcttaaag
1021 aacgagaaag acgtttaaag atgtgggagc aaaagctgac agagcagtcc aacaccccgc
1081 ttctcttgcc tcttgctgca agaatgtctg aggagtctta ctttgaatct aaaacagagg
1141 agtcaaacag tgcagagatg tcatgtcaga tcacagcaac aagtaacggg gagggccatg
1201 gcatgaaccc aagtctgcag gccatgatgc tgatgggctt tggggatatc ttctcaatga
1261 acaaagcagg agctgtgatg cattctggga tgcagataaa catgcaagcc aagcagaatt
1321 cttccaaaac cacatctaag agaaggggga agaaagtcaa catggctctg gggttcagtg
1381 attttgactt gtcagaaggt gacgatgatg atgatgatga cggtgaggag gaggataatg
1441 acatggataa tagtgaatga aagcagaaag caaagtaata aaatcacaaa tgtttggaaa
1501 acacaaaagt aacttgttta tctcagtctg tacaaaaaca gtaaggaggc agaaagccaa
1561 gcactgcatt tttaggccaa tcacatttac atgaccgtaa tttcttatca attctacttt
1621 tatttttgct tacagaaaaa cggggggaga attaagccaa agaagtatat ttatgaatca
1681 gcaaatgtgg tgcctgatta tagaaatttg tgatcctata tacaatatag gacttttaaa
1741 gttgtgacat tctggctttt tcttttaatg aatacttttt agtttgtatt tgactttatt
1801 tcctttattc aaatcatttt taaaaactta cattttgaac aaacactctt aactcctaat
1861 tgttctttga cacatagtaa ttctgtgaca tac
//