LOCUS AK225372 3569 bp mRNA linear HUM 17-NOV-2007
DEFINITION Homo sapiens mRNA for Leucyl-tRNA synthetase, cytoplasmic variant,
clone: HEP18172.
ACCESSION AK225372
VERSION AK225372.1
KEYWORDS FLI_CDNA; oligo capping.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3569)
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2006) to the DDBJ/EMBL/GenBank databases.
Contact:Akiko Tanaka
RIKEN Yokohama Institute, Protein Research Group; 1-7-22 Suehiro,
Tsurumi, Yokohama, Kanagawa 230-0045, Japan
URL :http://protein.gsc.riken.jp/
REFERENCE 2
AUTHORS Suzuki,Y., Sugano,S., Totoki,Y., Toyoda,A., Takeda,T., Sakaki,Y.,
Tanaka,A. and Yokoyama,S.
TITLE Homo sapiens protein coding cDNA.
JOURNAL Published Only in Database(2006)
REFERENCE 3
AUTHORS Maruyama,K. and Sugano,S.
TITLE Oligo-capping : a simple method to replace the cap structure of
eukaryotic mRNAs with oligoribonucleotides.
JOURNAL Gene 138, 171-174 (1994)
REFERENCE 4
AUTHORS Suzuki,Y., Yoshitomo,K., Maruyama,K., Suyama,A. and Sugano,S.
TITLE Construction and characterization of a full length-enriched and a
5'-end-enriched cDNA library .
JOURNAL Gene 200, 149-156 (1997)
COMMENT This work was supported in part by the National Project on Protein
Structural and Functional Analysis, Ministry of Education,
Culture, Sports, Science and Technology of Japan.
Sumio Sugano, Yutaka Suzuki
Laboratory of Functional Genomics Department of Medical Genome
Sciences Graduate School of Frontier Sciences The University of
Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 Japan
URL: http://www.k.u-tokyo.ac.jp/index.html.en
FEATURES Location/Qualifiers
source 1..3569
/cell_line="HepG2"
/cell_type="hepatoma"
/clone="HEP18172"
/clone_lib="HEP"
/db_xref="H-InvDB:HIT000384502"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="this clone is also named as hss001101730"
/organism="Homo sapiens"
/tissue_type="liver"
misc_feature 2..3523
/product="Leucyl-tRNA synthetase, cytoplasmic variant"
BASE COUNT 1128 a 652 c 838 g 951 t
ORIGIN
1 aagaaaagga acagccaaag tggacttttt gaagaagatt gagaaagaaa tccaacagaa
61 atgggatact gagagagtgt ttgaggtcaa tgcatctaat ttagagaaac agaccagcaa
121 gggcaagtat tttgtaacct tcccatatcc atatatgaat ggacgccttc atttgggaca
181 cacgttttct ttatccaaat gtgagtttgc tgtagggtac cagcgattga aaggaaaatg
241 ttgtctgttt ccctttggcc tgcactgtac tggaatgcct attagggcat gtgctgataa
301 gttgaaaaga gaaatagagc tgtatggttg cccccctgat tttccagatg aagaagagga
361 agaggaagaa accagtgtta aaacagaaga tataataatt aaggataaag ctaaaggaaa
421 aaagagtaaa gctgctgcta aagctggatc ttctaaatac cagtggggca ttatgaaatc
481 ccttggcctg tctgatgaag agatagtaaa attttctgaa gcagaacatt ggcttgatta
541 tttcccgcca ctggctattc aggatttaaa aagaatgggt ttgaaggtag actggcgtcg
601 ttccttcatc accactgatg ttaatcctta ctatgattca tttgtcagat ggcaattttt
661 aacattaaga gaaagaaaca aaattaaatt tgggaagcgg tatacaattt actctccgaa
721 agatggacag ccttgcatgg atcatgatag acaaactgga gagggtgttg gacctcagga
781 atatacttta ctcaaattga aggtgcttga gccataccca tctaaattaa gtggcctgaa
841 aggtaaaaat attttcttgg tggctgctac tctcagacct gagaccatgt ttgggcagac
901 aaattgttgg gttcgtcctg atatgaagta cattggattt gagacggtga atggtgatat
961 attcatctgt acccaaaaag cagccaggaa tatgtcatac cagggcttta ccaaagacaa
1021 tggcgtggtg cctgttgtta aggaattaat gggggaggaa attcttggtg catcactttc
1081 tgcaccttta acatcataca aggtgatcta tgttctccca atgctaacta ttaaggagga
1141 taaaggcact ggtgtggtta caagtgttcc ttccgactcc cctgatgata ttgctgccct
1201 cagagacttg aagaaaaagc aagccttacg agcaaaatat ggaattagag atgacatggt
1261 cttgccattt gagccggtgc cagtcattga aatcccaggt tttggaaatc tttctgctgt
1321 aaccatttgt gatgagttga aaattcagag ccagaatgac cgggaaaaac ttgcagaagc
1381 aaaggagaag atatatctaa aaggatttta tgagggtatc atgttggtgg atggatttaa
1441 aggacagaag gttcaagatg taaagaagac tattcagaaa aagatgattg acgctggaga
1501 tgcacttatt tacatggaac cagagaaaca agtgatgtcc aggtcgtcag atgaatgtgt
1561 tgtggctctg tgtgaccagt ggtacttgga ttatggagaa gagaattgga agaaacagac
1621 atctcagtgc ttgaagaacc tggaaacatt ctgtgaggag accaggagga attttgaagc
1681 caccttaggt tggctacaag aacatgcttg ctcaagaact tatggtctag gcactcacct
1741 gccttgggat gagcagtggc tgattgaatc actttctgac tccactattt acatggcatt
1801 ttacacagtt gcacacctat tgcagggggg taacttgcat ggacaggcag agtctccgct
1861 gggcattaga ccgcaacaga tgaccaagga agtttgggat tatgttttct tcaaggaggc
1921 tccatttcct aagactcaga ttgcaaagga aaaattagat cagttaaagc aggagtttga
1981 attctggtat cctgttgatc ttcgcgtctc tggcaaggat cttgttccaa atcatctttc
2041 atattacctt tataatcatg tggctatgtg gccggaacaa agtgacaaat ggcctacagc
2101 tgtgagagca aatggacatc tcctcctgaa ctctgagaag atgtcaaaat ccacaggcaa
2161 cttcctcact ttgacccaag ctattgacaa attttcagca gatggaatgc gtttggctct
2221 ggctgatgct ggtgacactg tagaagatgc caactttgtg gaagccatgg cagatgcagg
2281 tattctccgt ctgtacacct gggtagagtg ggtgaaagaa atggttgcca actgggacag
2341 cctaagaagt ggtcctgcca gcactttcaa tgatagagtt tttgccagtg aattgaatgc
2401 aggaattata aaaacagatc aaaactatga aaagatgatg tttaaagaag ctttgaaaac
2461 agggtttttt gagtttcagg ccgcaaaaga taagtaccgt gaattggctg tggaagggat
2521 gcacagagaa cttgtgttcc ggtttattga agttcagaca cttctcctcg ctccattctg
2581 tccacatttg tgtgagcaca tctggacact cctgggaaag cctgactcaa ttatgaatgc
2641 ttcatggcct gtggcaggtc ctgttaatga agttttaata cactcctcac agtatcttat
2701 ggaagtaaca catgacctta gactacgact caagaactat atgatgccag ctaaagggaa
2761 gaagactgac aaacaacccc tgcagaagcc ctcacattgc accatctatg tggcaaagaa
2821 ctatccacct tggcaacata ccaccctgtc tgttctacgt aaacactttg aggccaataa
2881 cggaaaactg cctgacaaca aagtcattgc tagtgaacta ggcagtatgc cagaactgaa
2941 gaaatacatg aagaaagtca tgccatttgt tgccatgatt aaggaaaatc tggagaagat
3001 ggggcctcgt attctggatt tgcaattaga atttgatgaa aaggctgtgc ttatggagaa
3061 tatagtctat ctgactaatt cgcttgagct agaacacata gaagtcaagt ttgcctccga
3121 agcagaagat aaaatcaggg aagactgctg tcctgggaaa ccacttaatg tttttagaat
3181 agaacctggt gtgtccgttt ctctggtgaa tccccagcca tccaatggcc acttctcaac
3241 caaaattgaa atcaggcaag gagataactg tgattccata atcaggcgtt taatgaaaat
3301 gaatcgagga attaaagacc tttccaaagt gaaactgatg agatttgatg atccactgtt
3361 ggggcctcga cgagttcctg tcctgggaaa ggagtacacc gagaagaccc ccatttctga
3421 gcatgctgtt ttcaatgtgg acctcatgag caagaaaatt catctgactg agaatgggat
3481 aagggtggat attggcgata caataatcta tctggttcat taaactcatg cacattggaa
3541 aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
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