LOCUS AK157380 959 bp mRNA linear HTC 06-OCT-2010
DEFINITION Mus musculus activated spleen cDNA, RIKEN full-length enriched
library, clone:F830212P03 product:immunoglobulin kappa chain
variable 28 (V28), full insert sequence.
ACCESSION AK157380
VERSION AK157380.1
KEYWORDS HTC_FLI; HTC; CAP trapper.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 959)
AUTHORS Arakawa,T., Carninci,P., Fukuda,S., Hashizume,W., Hayashida,K.,
Hori,F., Iida,J., Imamura,K., Imotani,K., Itoh,M., Kanagawa,S.,
Kawai,J., Kojima,M., Konno,H., Murata,M., Nakamura,M.,
Ninomiya,N., Nishiyori,H., Nomura,K., Ohno,M., Sakazume,N.,
Sano,H., Sasaki,D., Shibata,K., Shiraki,T., Tagami,M., Tagami,Y.,
Waki,K., Watahiki,A., Muramatsu,M. and Hayashizaki,Y.
TITLE Direct Submission
JOURNAL Submitted (30-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Yoshihide Hayashizaki
The Institute of Physical and Chemical Research (RIKEN), Omics
Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho,
Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
URL :http://www.osc.riken.jp/
REFERENCE 2
AUTHORS
CONSRTM The FANTOM Consortium, Riken Genome Exploration Research Group and
Genome Science Group (Genome Network Project Core Group)
TITLE The Transcriptional Landscape of the Mammalian Genome
JOURNAL Science 309, 1559-1563 (2005)
REFERENCE 3
AUTHORS
CONSRTM RIKEN Genome Exploration Research Group and Genome Science Group
(Genome Network Project Core Group) and the FANTOM Consortium
TITLE Antisense Transcription in the Mammalian Transcriptome
JOURNAL Science 309, 1564-1566 (2005)
REFERENCE 4
AUTHORS
CONSRTM The FANTOM Consortium and the RIKEN Genome Exploration Research
Group Phase I and II Team
TITLE Analysis of the mouse transcriptome based on functional annotation
of 60,770 full-length cDNAs
JOURNAL Nature 420, 563-573 (2002)
REFERENCE 5
AUTHORS
CONSRTM The RIKEN Genome Exploration Research Group Phase II Team and the
FANTOM Consortium
TITLE Functional annotation of a full-length mouse cDNA collection
JOURNAL Nature 409, 685-690 (2001)
REFERENCE 6
AUTHORS Carninci,P. and Hayashizaki,Y.
TITLE High-efficiency full-length cDNA cloning
JOURNAL Meth. Enzymol. 303, 19-44 (1999)
REFERENCE 7
AUTHORS Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K.,
Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y.
TITLE Normalization and subtraction of cap-trapper-selected cDNAs to
prepare full-length cDNA libraries for rapid discovery of new genes
JOURNAL Genome Res. 10, 1617-1630 (2000)
REFERENCE 8
AUTHORS Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N.,
Carninci,P., Konno,H., Akiyama,J., Nishi,K., Kitsunai,T.,
Tashiro,H., Itoh,M., Sumi,N., Ishii,Y., Nakamura,S., Hazama,M.,
Nishine,T., Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S.,
Ikegami,T., Kashiwagi,K., Fujiwake,S., Inoue,K., Togawa,Y.,
Izawa,M., Ohara,E., Watahiki,M., Yoneda,Y., Ishikawa,T., Ozawa,K.,
Tanaka,T., Matsuura,S., Kawai,J., Okazaki,Y., Muramatsu,M.,
Inoue,Y., Kira,A. and Hayashizaki,Y.
TITLE RIKEN integrated sequence analysis (RISA) system-384-format
sequencing pipeline with 384 multicapillary sequencer
JOURNAL Genome Res. 10, 1757-1771 (2000)
COMMENT cDNA library was prepared and sequenced in Mouse Genome
Encyclopedia Project of Genome Exploration Research Group in Riken
Genomic Sciences Center and Genome Science Laboratory in RIKEN.
Division of Experimental Animal Research in Riken contributed to
prepare mouse tissues.
Tissues were provided by Dr. John Todd (Dept. of Medical Genetics
Wellcome Trust Centre for Molecular Mechanisms in Disease Wellcome
Trust/MRC building Addenbrookes Hospital Cambridge) whose
assistance we gratefully acknowledge.
Please visit our web site for further details.
URL:http://www.osc.riken.jp/
URL:http://fantom.gsc.riken.jp/
clone information is available at:
http://fantom.gsc.riken.jp/3/db/annotate/
main.cgi?masterid=F830212P03
FEATURES Location/Qualifiers
source 1..959
/clone="F830212P03"
/clone_lib="RIKEN full-length enriched mouse cDNA library"
/db_xref="FANTOM_DB:F830212P03"
/db_xref="MGI:3558622"
/db_xref="taxon:10090"
/mol_type="mRNA"
/organism="Mus musculus"
/strain="NOD"
/tissue_type="activated spleen"
CDS 32..751
/codon_start=1
/note="immunoglobulin kappa chain variable 28 (V28)
(MGD|MGI:96511 GB|AK002514, evidence: BLASTN, 98%,
match=560)"
/note="putative"
/protein_id="BAE34073.1"
/transl_table=1
/translation="MRCSLQFLGMLMFWISGVSGDIVITQDELSNPVTSGESVSISCR
SSKSLLYKDGKTYLNWFLQRPGQSPQLLVYWMSTRASGVSDRFSGSGSGTDFTLEISR
VKAEDVGVYYCQQVVEYPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCF
LNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYT
CEATHKTSTSPIVKSFNRNEC"
regulatory 937..942
/note="putative"
/regulatory_class="polyA_signal_sequence"
polyA_site 959
/note="putative"
BASE COUNT 245 a 250 c 218 g 246 t
ORIGIN
1 gtatcagaac tcttcacacc aagttctcag aatgaggtgc tctcttcagt tcttggggat
61 gcttatgttc tggatctctg gagtcagtgg ggatattgtg ataacccagg atgaactctc
121 caatcctgtc acttctggag aatcagtttc catctcctgc aggtctagta agagtctcct
181 atataaggat gggaagacat acttgaattg gtttctgcag aggccaggac agtctcctca
241 gctcctggtc tattggatgt ccacccgtgc atcaggagtc tcagaccggt ttagtggcag
301 tgggtcagga acagatttca cactggaaat cagtagagtg aaggctgagg atgtcggtgt
361 gtattactgt caacaagttg tagagtatcc tctcacgttc ggtgctggga ccaagctgga
421 gctgaaacgg gctgatgctg caccaactgt atccatcttc ccaccatcca gtgagcagtt
481 aacatctgga ggtgcctcag tcgtgtgctt cttgaacaac ttctacccca aagacatcaa
541 tgtcaagtgg aagattgatg gcagtgaacg acaaaatggc gtcctgaaca gttggactga
601 tcaggacagc aaagacagca cctacagcat gagcagcacc ctcacgttga ccaaggacga
661 gtatgaacga cataacagct atacctgtga ggccactcac aagacatcaa cttcacccat
721 cgtcaagagc ttcaacagga atgagtgtta gagacaaagg tcctgagatg ccaccaccag
781 ctccccagct ccatcctatc ttcccttcta aggtcttgga ggcttcccca caagcgacct
841 accactgttg cggtgctcca aacctcctcc ccacctcctt ctcctcctcc tccctttcct
901 tggcttttat catgctaata tttgcagaaa atattcaata aagtgagtct ttgcacttg
//