LOCUS AK154055 1439 bp mRNA linear HTC 06-OCT-2010 DEFINITION Mus musculus 2 days neonate thymus thymic cells cDNA, RIKEN full-length enriched library, clone:E430030F23 product:CD8 antigen, beta chain, full insert sequence. ACCESSION AK154055 VERSION AK154055.1 KEYWORDS HTC_FLI; HTC; CAP trapper. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 1439) AUTHORS Arakawa,T., Carninci,P., Fukuda,S., Hashizume,W., Hayashida,K., Hori,F., Iida,J., Imamura,K., Imotani,K., Itoh,M., Kanagawa,S., Kawai,J., Kojima,M., Konno,H., Murata,M., Nakamura,M., Ninomiya,N., Nishiyori,H., Nomura,K., Ohno,M., Sakazume,N., Sano,H., Sasaki,D., Shibata,K., Shiraki,T., Tagami,M., Tagami,Y., Waki,K., Watahiki,A., Muramatsu,M. and Hayashizaki,Y. TITLE Direct Submission JOURNAL Submitted (30-MAR-2004) to the DDBJ/EMBL/GenBank databases. Contact:Yoshihide Hayashizaki The Institute of Physical and Chemical Research (RIKEN), Omics Science Center, RIKEN Yokohama Institute; 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan URL :http://www.osc.riken.jp/ REFERENCE 2 AUTHORS CONSRTM The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) TITLE The Transcriptional Landscape of the Mammalian Genome JOURNAL Science 309, 1559-1563 (2005) REFERENCE 3 AUTHORS CONSRTM RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium TITLE Antisense Transcription in the Mammalian Transcriptome JOURNAL Science 309, 1564-1566 (2005) REFERENCE 4 AUTHORS CONSRTM The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team TITLE Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs JOURNAL Nature 420, 563-573 (2002) REFERENCE 5 AUTHORS CONSRTM The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium TITLE Functional annotation of a full-length mouse cDNA collection JOURNAL Nature 409, 685-690 (2001) REFERENCE 6 AUTHORS Carninci,P. and Hayashizaki,Y. TITLE High-efficiency full-length cDNA cloning JOURNAL Meth. Enzymol. 303, 19-44 (1999) REFERENCE 7 AUTHORS Carninci,P., Shibata,Y., Hayatsu,N., Sugahara,Y., Shibata,K., Itoh,M., Konno,H., Okazaki,Y., Muramatsu,M. and Hayashizaki,Y. TITLE Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes JOURNAL Genome Res. 10, 1617-1630 (2000) REFERENCE 8 AUTHORS Shibata,K., Itoh,M., Aizawa,K., Nagaoka,S., Sasaki,N., Carninci,P., Konno,H., Akiyama,J., Nishi,K., Kitsunai,T., Tashiro,H., Itoh,M., Sumi,N., Ishii,Y., Nakamura,S., Hazama,M., Nishine,T., Harada,A., Yamamoto,R., Matsumoto,H., Sakaguchi,S., Ikegami,T., Kashiwagi,K., Fujiwake,S., Inoue,K., Togawa,Y., Izawa,M., Ohara,E., Watahiki,M., Yoneda,Y., Ishikawa,T., Ozawa,K., Tanaka,T., Matsuura,S., Kawai,J., Okazaki,Y., Muramatsu,M., Inoue,Y., Kira,A. and Hayashizaki,Y. TITLE RIKEN integrated sequence analysis (RISA) system-384-format sequencing pipeline with 384 multicapillary sequencer JOURNAL Genome Res. 10, 1757-1771 (2000) COMMENT cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science Laboratory in RIKEN. Division of Experimental Animal Research in Riken contributed to prepare mouse tissues. Please visit our web site for further details. URL:http://www.osc.riken.jp/ URL:http://fantom.gsc.riken.jp/ clone information is available at: http://fantom.gsc.riken.jp/3/db/annotate/ main.cgi?masterid=E430030F23 FEATURES Location/Qualifiers source 1..1439 /cell_type="thymic cells" /clone="E430030F23" /clone_lib="RIKEN full-length enriched mouse cDNA library" /db_xref="FANTOM_DB:E430030F23" /db_xref="MGI:3551561" /db_xref="taxon:10090" /dev_stage="2 days neonate" /mol_type="mRNA" /organism="Mus musculus" /strain="NOD" /tissue_type="thymus" CDS 26..667 /codon_start=1 /note="CD8 antigen, beta chain (MGD|MGI:88347 GB|AK087944, evidence: BLASTN, 99%, match=1429)" /note="putative" /protein_id="BAE32345.1" /transl_table=1 /translation="MQPWLWLVFSMKLAALWSSSALIQTPSSLLVQTNHTAKMSCEVK SISKLTSIYWLRERQDPKDKYFEFLASWSSSKGVLYGESVDKKRNIILESSDSSRPFL SIMNVKPEDSDFYFCATVGSPKMVFGTGTKLTVVDVLPTTAPTKKTTLKMKKKKQCPF PHPETQKGLTCSLTTLSLLVVCILLLLAFLGVAVYFYCVRRRARIHFMKQFHK" BASE COUNT 360 a 383 c 351 g 345 t ORIGIN 1 gaacagtgtc cccaaaagcg ccaagatgca gccatggctc tggctggtct tcagtatgaa 61 gctggcagct ctctggagca gctctgccct cattcagacc ccttcgtccc tgctggttca 121 aaccaaccat acggcaaaga tgtcctgtga ggttaaaagc atctctaagt taacaagcat 181 ctactggctg cgggagcgcc aggaccccaa ggacaagtac tttgagttcc tggcctcctg 241 gagttcttcc aaaggagttt tgtatggtga aagtgtggac aagaaaagaa atataattct 301 tgagtcttca gactcaagcc ggccctttct cagtatcatg aatgtgaagc cagaggacag 361 tgacttctac ttctgcgcga cggttgggag ccccaagatg gtctttggga cagggacgaa 421 gctgactgtg gttgatgtcc ttcctacaac tgccccaacc aagaagacta ccctgaagat 481 gaagaagaag aagcaatgcc cgttccccca cccagagacc cagaagggcc tgacatgcag 541 ccttaccacc ctcagcctgc tggtagtctg catcctgctt ctgctggcat tcctcggagt 601 ggccgtctac ttttactgtg tgcggaggag agcccgaatt cacttcatga aacagtttca 661 caaatgatca gcgcccacaa cactgatagc ctgctagcaa taaaaggact gtaactcaat 721 caaagcaaga tctggactag tgagagacag cgcattcagg actggagagg cccttgccct 781 tgctcaagct gcctgctttg aactgctgca agctctgtgt gtggtttggg gtctcacaag 841 gaaggcctca gaacccaagc tcacgcacag agtggctgaa gaaccaagaa ctcccacgcc 901 accatgtact cttcttggac tgggccttgg acagtggcct tccagccact gtctttgtca 961 gttgcttggt aggacagcct tattgactct ttggcctttc atggaaaaga gctagccggg 1021 cagccctgga gaagactgca aatcctcaca cagagaagcc acctcatgaa taaggtttcc 1081 tgcaaacagt ttctgggctc tcagcagcca tcttactttc tcaggccttg ccgtgggttt 1141 gggaggccgt tggctcagga gacagacttc tacttcatct cctagtagga agtttcttaa 1201 gcttcacttc ttctctctcg tggcttatat atttcttctc tcactctctg cccttttaat 1261 gaaaatgttt tggctataaa aataaagtcc tgccgggcat ggtggtgcat gcctttaatc 1321 ccagcactca ggaggcagag gcaggcggat ctctgagttc gaggccagcc tggtctacag 1381 agtgagttcc aggacagcca aggctacaga gaaaccctgt ctcaaaaaaa cacaaaaac //