LOCUS AK055051 2550 bp mRNA linear HUM 09-JAN-2008
DEFINITION Homo sapiens cDNA FLJ30489 fis, clone BRAWH2000142, highly similar
to Rattus norvegicus vesicular GABA transporter (VGAT) mRNA.
ACCESSION AK055051
VERSION AK055051.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2550)
AUTHORS Isogai,T., Otsuki,T. and Sugiyama,T.
TITLE Direct Submission
JOURNAL Submitted (24-OCT-2001) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Nishi,T., Nakagawa,S., Senoh,A., Mizuguchi,H., Inagaki,H.,
Sugiyama,T., Irie,R., Otsuki,T., Sato,H., Ota,T., Wakamatsu,A.,
Ishii,S., Yamamoto,J., Isono,Y., Kawai-Hio,Y., Saito,K.,
Nishikawa,T., Kimura,K., Yamashita,H., Matsuo,K., Nakamura,Y.,
Sekine,M., Kikuchi,H., Kanda,K., Wagatsuma,M., Murakawa,K.,
Kanehori,K., Takahashi-Fujii,A., Oshima,A., Sugiyama,A.,
Kawakami,B., Suzuki,Y., Sugano,S., Nagahari,K., Masuho,Y.,
Nagai,K. and Isogai,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2001)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
Economy, Trade and Industry of Japan; cDNA full insert sequencing:
Research Association for Biotechnology (RAB); cDNA library
construction: Helix Research Institute (HRI) (supported by Japan
Key Technology Center etc.); 5'- & 3'-end one pass sequencing:
RAB, HRI, and Biotechnology Center, National Institute of
Technology and Evaluation; clone selection for full insert
sequencing: RAB and HRI.
FEATURES Location/Qualifiers
source 1..2550
/clone="BRAWH2000142"
/clone_lib="BRAWH2"
/db_xref="H-InvDB:HIT000011665"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/organism="Homo sapiens"
/tissue_type="brain"
CDS 240..1817
/codon_start=1
/protein_id="BAB70846.1"
/translation="MATLLRSKLSNVATSVSNKSQAKMSGMFARMGFQAATDEEAVGF
AHCDDLDFEHRQGLQMDILKAEGEPCGDEGAEAPVEGDIHYQRGSGAPLPPSGSKDQV
GGGGEFGGHDKPKITAWEAGWNVTNAIQGMFVLGLPYAILHGGYLGLFLIIFAAVVCC
YTGKILIACLYEENEDGEVVRVRDSYVAIANACCAPRFPTLGGRVVNVAQIIELVMTC
ILYVVVSGNLMYNSFPGLPVSQKSWSIIATAVLLPCAFLKNLKAVSKFSLLCTLAHFV
INILVIAYCLSRARDWAWEKVKFYIDVKKFPISIGIIVFSYTSQIFLPSLEGNMQQPS
EFHCMMNWTHIAACVLKGLFALVAYLTWADETKEVITDNLPGSIRAVVNIFLVAKALL
SYPLPFFAAVEVLEKSLFQEGSRAFFPACYSGDGRLKSWGLTLRCALVVFTLLMAIYV
PHFALLMGLTGSLTGAGLCFLLPSLFHLRLLWRKLLWHQVFFDVAIFVIGGICSVSGF
VHSLEGLIEAYRTNAED"
BASE COUNT 450 a 803 c 758 g 539 t
ORIGIN
1 agtgcactag ccaccaccgc cgccgccgcc gctccgccag acctgctgcc atcttgcccg
61 gtccagccct gagagagcct cgaacgccag ctgcgagggt catgagccag agagccccgg
121 ggcgccgcgc ggagagcaag cggagatagc gactttgcgc cccccagccc tcgcctcctt
181 gcatcgcgtt ccccgcatcc tcgggtcctt ctgtcctttc cgctgtcccc accgccgcca
241 tggccacctt gctccgcagc aagctgtcca acgtggccac gtccgtgtcc aacaagtccc
301 aggccaagat gagcggcatg ttcgccagga tgggttttca ggcggccacg gatgaggagg
361 cggtgggctt cgcgcattgc gacgacctcg actttgagca ccgccagggc ctgcagatgg
421 acatcctgaa agccgaggga gagccctgcg gggacgaggg cgctgaagcg cccgtcgagg
481 gagacatcca ttatcagcga ggcagcggag ctcctctgcc gccctccggc tccaaggacc
541 aggtgggagg tggtggcgaa ttcgggggcc acgacaagcc caaaatcacg gcgtgggagg
601 caggctggaa cgtgaccaac gccatccagg gcatgttcgt gctgggccta ccctacgcca
661 tcctgcacgg cggctacctg gggttgtttc tcatcatctt cgccgccgtt gtgtgctgct
721 acaccggcaa gatcctcatc gcgtgcctgt acgaggagaa tgaagacggc gaggtggtgc
781 gcgtgcggga ctcgtacgtg gccatagcca acgcctgctg cgccccgcgc ttcccaacgc
841 tgggcggccg agtggtgaac gtagcgcaga tcatcgagct ggtgatgacg tgcatcctgt
901 acgtggtggt gagtggcaac ctcatgtaca acagcttccc ggggctgccc gtgtcgcaga
961 agtcctggtc cattatcgcc acggccgtgc tgctgccttg cgccttcctt aagaacctca
1021 aggccgtgtc caagttcagt ctgctgtgca ctctggccca cttcgtcatc aatatcctgg
1081 tcatagccta ctgtctatcg cgggcgcgcg actgggcctg ggagaaggtc aagttctaca
1141 tcgacgtcaa gaagttcccc atctccattg gcatcatcgt gttcagctac acgtctcaga
1201 tcttcctgcc ttcgctggag ggcaatatgc agcagcccag cgagttccac tgcatgatga
1261 actggacgca catcgcagcc tgcgtgctca agggcctctt cgcgctcgtc gcctacctca
1321 cctgggccga cgagaccaag gaggtcatca cggataacct gcccggctcc atccgcgccg
1381 tggtcaacat ctttctggtg gccaaggcgc tgttgtccta tcctctgcca ttctttgccg
1441 ctgtcgaggt gctggagaag tcgctcttcc aggaaggcag ccgcgccttt ttcccggcct
1501 gctacagcgg cgacgggcgc ctgaagtcct gggggctgac gctgcgctgc gcgctcgtcg
1561 tcttcacgct gctcatggcc atttatgtgc cgcacttcgc gctgctcatg ggcctcaccg
1621 gcagcctcac gggcgccggc ctctgtttct tgctgcccag cctctttcac ctgcgcctgc
1681 tctggcgcaa gctgctgtgg caccaagtct tcttcgacgt cgccatcttc gtcatcggcg
1741 gcatctgcag cgtgtccggc ttcgtgcact ccctcgaggg cctcatcgaa gcctaccgaa
1801 ccaacgcgga ggactagggc gcaagggcga gcccccgccg cgcttctgcg ctctctccct
1861 tctcccctca ccccgccccc accagcccag tgcgccctgc cgccgcgctt gggaggccaa
1921 gctttaaaca tctctggttc ctagtttctg attattcggg gatggggggg atgggagggg
1981 acagggattc acgatccatc gcgtctgcgt ttctgttgtc ctttcttttc cacaacaccc
2041 tggttttggg gggaggcggg gtgcatttgc gggcagggtt ctctgtcctt ccaagtgggg
2101 ccccgacact ttggttccag tcatcgaggg ggttgggaag ggagggagag ggggcgcagc
2161 tcgcaggcgt ggcaacttga ccttggggga atatttcaca tccatccaga gctcggaatc
2221 tacagcgtcc agccatttcc agcaagagcg cttcccattc cggagacgtt tcaaccctgc
2281 agcgggaaag gctgactggg aaatccattt tgggtgggca atttccttca acgaagccgg
2341 aaggcgagaa gccgcggcgg ggccagcttg cctgccggtt ttcaggaatc taaactctca
2401 tcttgtgcaa tttatcaggt gtggaactgt tctactgtgc gtgtggtgtg ctcgtggtga
2461 ataagatgaa atgtatatca gaaaaaaatc tatctctaat ttagagtgcg gtacataatt
2521 atatccgcaa ataaagaaga gacaaaggct
//