LOCUS AK001386 2738 bp mRNA linear HUM 03-JUL-2008
DEFINITION Homo sapiens cDNA FLJ10524 fis, clone NT2RP2000880, highly similar
to Eukaryotic translation initiation factor 5B.
ACCESSION AK001386
VERSION AK001386.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2738)
AUTHORS Isogai,T. and Otsuki,T.
TITLE Direct Submission
JOURNAL Submitted (16-FEB-2000) to the DDBJ/EMBL/GenBank databases.
Contact:Takao Isogai
Helix Research Institute, Genomics Laboratory; 1532-3 Yana,
Kisarazu, Chiba 292-0812, Japan
E-mail :flj-cdna@nifty.com
REFERENCE 2
AUTHORS Ota,T., Suzuki,Y., Nishikawa,T., Otsuki,T., Sugiyama,T., Irie,R.,
Wakamatsu,A., Hayashi,K., Sato,H., Nagai,K., Kimura,K., Makita,H.,
Sekine,M., Obayashi,M., Nishi,T., Shibahara,T., Tanaka,T.,
Ishii,S., Yamamoto,J., Saito,K., Kawai,Y., Isono,Y., Nakamura,Y.,
Nagahari,K., Murakami,K., Yasuda,T., Iwayanagi,T., Wagatsuma,M.,
Shiratori,A., Sudo,H., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Kanda,K., Yokoi,T., Furuya,T.,
Kikkawa,E., Omura,Y., Abe,K., Kamihara,K., Katsuta,N., Sato,K.,
Tanikawa,M., Yamazaki,M., Ninomiya,K., Ishibashi,T., Yamashita,H.,
Murakawa,K., Fujimori,K., Tanai,H., Kimata,M., Watanabe,M.,
Hiraoka,S., Chiba,Y., Ishida,S., Ono,Y., Takiguchi,S.,
Watanabe,S., Yosida,M., Hotuta,T., Kusano,J., Kanehori,K.,
Takahashi-Fujii,A., Hara,H., Tanase,T., Nomura,Y., Togiya,S.,
Komai,F., Hara,R., Takeuchi,K., Arita,M., Imose,N., Musashino,K.,
Yuuki,H., Oshima,A., Sasaki,N., Aotsuka,S., Yoshikawa,Y.,
Matsunawa,H., Ichihara,T., Shiohata,N., Sano,S., Moriya,S.,
Momiyama,H., Satoh,N., Takami,S., Terashima,Y., Suzuki,O.,
Nakagawa,S., Senoh,A., Mizoguchi,H., Goto,Y., Shimizu,F.,
Wakebe,H., Hishigaki,H., Watanabe,T., Sugiyama,A., Takemoto,M.,
Kawakami,B., Yamazaki,M., Watanabe,K., Kumagai,A., Itakura,S.,
Fukuzumi,Y., Fujimori,Y., Komiyama,M., Tashiro,H., Tanigami,A.,
Fujiwara,T., Ono,T., Yamada,K., Fujii,Y., Ozaki,K., Hirao,M.,
Ohmori,Y., Kawabata,A., Hikiji,T., Kobatake,N., Inagaki,H.,
Ikema,Y., Okamoto,S., Okitani,R., Kawakami,T., Noguchi,S.,
Itoh,T., Shigeta,K., Senba,T., Matsumura,K., Nakajima,Y.,
Mizuno,T., Morinaga,M., Sasaki,M., Togashi,T., Oyama,M., Hata,H.,
Watanabe,M., Komatsu,T., Mizushima-Sugano,J., Satoh,T., Shirai,Y.,
Takahashi,Y., Nakagawa,K., Okumura,K., Nagase,T., Nomura,N.,
Kikuchi,H., Masuho,Y., Yamashita,R., Nakai,K., Yada,T.,
Nakamura,Y., Ohara,O., Isogai,T. and Sugano,S.
TITLE Complete sequencing and characterization of 21,243 full-length
human cDNAs
JOURNAL Nat. Genet. 36, 40-45 (2004)
REFERENCE 3
AUTHORS Isogai,T., Ota,T., Hayashi,K., Sugiyama,T., Otsuki,T., Suzuki,Y.,
Nishikawa,T., Nagai,K., Sugano,S., Shiratori,A., Sudo,H.,
Wagatsuma,M., Hosoiri,T., Kaku,Y., Kodaira,H., Kondo,H.,
Sugawara,M., Takahashi,M., Chiba,Y., Ishida,S., Murakawa,K.,
Ono,Y., Takiguchi,S., Watanabe,S., Kimura,K., Murakami,K.,
Ishii,S., Kawai,Y., Saito,K., Yamamoto,J., Wakamatsu,A.,
Nakamura,Y., Nagahari,K., Masuho,Y., Ninomiya,K. and Iwayanagi,T.
TITLE NEDO human cDNA sequencing project
JOURNAL Unpublished (2000)
COMMENT NEDO human cDNA sequencing project supported by Ministry of
International Trade and Industry of Japan; cDNA full insert
sequencing: Research Association for Biotechnology; cDNA library
construction, 5'- & 3'-end one pass sequencing and clone
selection: Helix Research Institute (supported by Japan Key
Technology Center etc.) and Department of Virology, Institute of
Medical Science, University of Tokyo.
FEATURES Location/Qualifiers
source 1..2738
/cell_line="NT2"
/cell_type="teratocarcinoma"
/clone="NT2RP2000880"
/clone_lib="NT2RP2"
/db_xref="H-InvDB:HIT000003860"
/db_xref="taxon:9606"
/mol_type="mRNA"
/note="cloning vector: pME18SFL3"
/note="mRNA from NT2 neuronal precursor cells after
2-weeks retinoic acid (RA) induction."
/organism="Homo sapiens"
CDS 181..2085
/codon_start=1
/note="highly similar to Eukaryotic translation
initiation factor 5B"
/protein_id="BAG50898.1"
/translation="MSSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLR
APIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIENF
DRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKS
KKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLN
AALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVME
VKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYE
KHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEE
KGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILA
FDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKI
KILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAK
KGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLI
VELKKVFEII"
BASE COUNT 917 a 463 c 620 g 738 t
ORIGIN
1 gtaaaagaaa accctgaaga ggaggaggag gaggaagaag aggaagaaga agatgaagaa
61 agtgaagaag aggaggaaga ggagggagaa agtgaaggca gtgaaggtga tgaggaagat
121 gaaaaggtgt cagatgagaa ggattcaggg aagacattag ataaaaagcc aagtaaagaa
181 atgagctcag attctgaata tgactctgat gatgatcgga ctaaagaaga aagggcttat
241 gacaaagcaa aacggaggat tgagaaacgg cgacttgaac atagtaaaaa tgtaaacacc
301 gaaaagctaa gagcccctat tatctgcgta cttgggcatg tggacacagg gaagacaaaa
361 attctagata agctccgtca cacacatgta caagacggtg aagcaggtgg tatcacacaa
421 caaattgggg ccaccaatgt tcctcttgaa gctattaatg aacagactaa gatgattgaa
481 aattttgata gagagaatgt acggattcca ggaatgctaa ttattgatac tcctgggcat
541 gaatctttca gtaatctgag aaatagagga agctctcttt gtgacattgc cattttagtt
601 gttgatatta tgcatggttt ggagccccag acaattgagt ctatcaacct tctcaaatct
661 aaaaaatgtc ccttcattgt tgcactcaat aagattgata ggttatatga ttggaaaaag
721 agtcctgact ctgatgtggc tgctacttta aagaagcaga aaaagaatac aaaagatgaa
781 tttgaggagc gagcaaaggc tattattgta gaatttgcac agcagggttt gaatgctgct
841 ttgttttatg agaataaaga tccccgcact tttgtgtctt tggtacctac ctctgcacat
901 actggtgatg gcatgggaag tctgatctac cttcttgtag agttaactca gaccatgttg
961 agcaagagac ttgcacactg tgaagagctg agagcacagg tgatggaggt taaagctctc
1021 ccggggatgg gcaccactat agatgtcatc ttgatcaatg ggcgtttgaa ggaaggagat
1081 acaatcattg ttcctggagt agaagggccc attgtaactc agattcgagg cctcctgtta
1141 cctcctccta tgaaggaatt acgagtgaag aaccagtatg aaaagcataa agaagtagaa
1201 gcagctcagg gggtaaagat tcttggaaaa gacctggaga aaacattggc tggtttaccc
1261 ctccttgtgg cttataaaga agatgaaatc cctgttctta aagatgaatt gatccatgag
1321 ttaaagcaga cactaaatgc tatcaaatta gaagaaaaag gagtctatgt ccaggcatct
1381 acgctgggtt ctttggaagc tctactggaa tttctgaaaa catcagaagt gccctatgca
1441 ggaattaaca ttggcccagt gcataaaaaa gatgttatga aggcttcagt gatgttggaa
1501 catgaccctc agtatgcagt aattttggcc ttcgatgtga gaattgaacg agatgcacaa
1561 gaaatggctg atagtttagg agttagaatt tttagtgcag aaattattta tcatttattt
1621 gatgccttta caaaatatag acaagactac aagaaacaga aacaagaaga atttaagcac
1681 atagcagtat ttccctgcaa gataaaaatc ctccctcagt acatttttaa ttctcgagat
1741 ccgatagtga tgggggtgac ggtggaagca ggtcaggtga aacaggggac acccatgtgt
1801 gtcccaagca aaaattttgt tgacatcgga atagtaacaa gtattgaaat aaaccataaa
1861 caagtggatg ttgcaaaaaa aggacaagaa gtttgtgtaa aaatagaacc tatccctggt
1921 gagtcaccca aaatgtttgg aagacatttt gaagctacag atattcttgt tagtaagatc
1981 agccggcagt ccattgatgc actcaaagac tggttcagag atgaaatgca gaagagtgac
2041 tggcagctta ttgtggagct gaagaaagta tttgaaatca tctaattttt tcacatggag
2101 caggaactgg agtaaatgca atactgtgtt gtaatatccc aacaaaaatc agacaaaaaa
2161 tggaacagac gtatttggac actgatggac ttaagtatgg aaggaagaaa aataggtgta
2221 taaaatgttt tccatgagaa accaagaaac ttacactggt ttgacagtgg tcagttacat
2281 gtccccacag ttccaatgtg cctgttcact cacctctccc ttccccaacc cttctctact
2341 tggctgctgt tttaaagttt gcccttcccc aaatttggat ttttattaca gatctaaagc
2401 tctttcgatt ttatactgat taaatcagta ctgcagtatt tgattaacca agcttctgca
2461 gattttgtga ttcttgggac ttttttgacg taagaaatac ttctttattt atgcatattc
2521 ttcccacagt gatttttcca gcattcttct gccatatgcc tttagggctt ttataaaata
2581 gaaaattagg cattctgata tttctttagc tgctttgtgt gaaaccatgg tgtaaaagca
2641 cagctggctg ctttttactg cttgtgtagt cacgagtcca ttgtaatcat cacaattcta
2701 aaccaaacta ccaataaaga aaacagacat ccaccagt
//