LOCUS       AI462655                 306 bp    mRNA    linear   EST 09-MAR-1999
DEFINITION  vb59g11.x1 Ko mouse embryo 11 5dpc Mus musculus cDNA clone
            IMAGE:761348 3', mRNA sequence.
VERSION     AI462655.1
DBLINK      BioSample: SAMN00155444
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Marra,M., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T.,
            Underwood,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y.,
            Person,B., Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R.,
            Ritter,E., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R.,
            Waterston,R. and Wilson,R.
  TITLE     The WashU-NCI Mouse EST Project 1999
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/WashU-NCI Mouse EST Project 1999
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium ( for further information.
            This clone was previously sequenced on the 5' end only, this new
            data is from the 3' end
            Seq primer: Primer name ambiguous.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Mus musculus"
                     /clone_lib="SAMN00155444 Ko mouse embryo 11 5dpc"
                     /note="Organ: embryo; Vector: pSPORT1; Site_1: SalI;
                     Site_2: NotI; Total RNAs were extracted from 11.5 dpc
                     embryos (excluding placenta and yolk sac). The
                     double-stranded cDNA was synthesized with an oligo (dT)-1
                     3'. The cDNAs were ligated to LL-Sal3A: 5'
                     3'. The cDNAs were size-selected and amplified by
                     long-range PCR using Ex Taq polymerase for 18 cycles. The
                     PCR-amplifiable cDNA mixture went through one round of
                     equalization and was digested with SalI/NotI and cloned
                     into the SalI/NotI sites of the pSPORT1 plasmid vector
                     (Life Technologies). The library was constructed by Dr.
                     Minoru S. H. Ko and Dr. Xiaohong Wang."
BASE COUNT          101 a           76 c           41 g           88 t
        1 ttttttctgt gtataacttt tatttccaat atagtaaata aagcaaaata aaaaccatta
       61 agaaattttg acagcatcta cccatttttc ttttctataa accccaaagt ggtgtgacaa
      121 ttcctttgcc cacatactca agtcttggag tacttaaaaa gtcaaaactc acgaatgctg
      181 ctaataagaa agtctgaaat ggtactgccc ttcacccaga aatagcacac actgagcctc
      241 attatccaag gggagaaaag ctggctccta gaacatattc cctcccctca cccacctccc
      301 ctttga