LOCUS AI200186 508 bp mRNA linear EST 08-JAN-2011 DEFINITION qf85g06.x1 Soares_fetal_lung_NbHL19W Homo sapiens cDNA clone IMAGE:1756858 3' similar to gb:V00572 PHOSPHOGLYCERATE KINASE 1 (HUMAN), mRNA sequence. ACCESSION AI200186 VERSION AI200186.1 DBLINK BioSample: SAMN00155027 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 508) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -40UP from Gibco High quality sequence stop: 241. FEATURES Location/Qualifiers source 1..508 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1756858" /clone_lib="SAMN00155027 Soares_fetal_lung_NbHL19W" /dev_stage="19 weeks" /lab_host="DH10B (ampicillin resistant)" /note="Organ: lung; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAATTTTTTTTTTTTTTTTTT-3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. This library was constructed from the same fetus as the fetal heart library, Soares fetal heart NbHH19W." BASE COUNT 158 a 101 c 107 g 142 t ORIGIN 1 tttttcaatg gacactttta ttgcttactt aatggatcat caattttgcc tcactaccta 61 caaatggaat ttcatcttgt ttccatgctg agtagtgaaa cagtgacaaa gctaatcata 121 ataacctaca tcaaaagaga actaagctaa cactgctcac tttcttttta acaggcaaaa 181 tataaatata tgcactctag aatgcacaat ggtttagtca ctaaaaaatt caaatgggat 241 cttgaagaat gtatgcaaat ccagggtgca gggaagatga gctgagatgc tgcgcaactg 301 tttaagggtt cctggcactg catctcttgg ccactagctg aatcttgaca tggaagggtt 361 tagcttatgc caagttggaa tgcaaaaaat gcttagttgg cttaagggct gggcacagga 421 acctaaaggg gggaaagtct taatattggt gtgagccttc accccaagga ggagctttcc 481 ttcacgactc tcaaatggag caccccca //