LOCUS AI127823 499 bp mRNA linear EST 05-JAN-2011
DEFINITION qc36f03.x1 Soares_pregnant_uterus_NbHPU Homo sapiens cDNA clone
IMAGE:1711709 3' similar to gb:V00572 PHOSPHOGLYCERATE KINASE 1
(HUMAN), mRNA sequence.
ACCESSION AI127823
VERSION AI127823.1
DBLINK BioSample: SAMN00155083
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 499)
CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
Tumor Gene Index
JOURNAL Unpublished
COMMENT Contact: Robert Strausberg, Ph.D.
Email: cgapbs-r@mail.nih.gov
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 695 Std Error: 0.00
Seq primer: -40m13 fwd. ET from Amersham
High quality sequence stop: 350.
FEATURES Location/Qualifiers
source 1..499
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:1711709"
/sex="female"
/clone_lib="SAMN00155083 Soares_pregnant_uterus_NbHPU"
/dev_stage="adult"
/lab_host="DH10B"
/note="Organ: uterus; Vector: pT7T3D-PacI; Site_1: Not I;
Site_2: Eco RI; 1st strand cDNA was primed with a Not I -
oligo(dT) primer [5'
AACTGGAAGAATTCGCGGCCGCCTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not I
and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization. Library
constructed by M. Fatima Bonaldo."
BASE COUNT 161 a 96 c 104 g 138 t
ORIGIN
1 cacggtttca atggacactt ttattgttta cttaatggat catcaatttt gtctcactac
61 ctacaaatgg aatttcatct tgtttccatg ctgagtagtg aaacagtgac aaagctaatc
121 ataataacct acatcaaaag agaactaagc taacactgct cactttcttt ttaacaggca
181 aaatataaat atatgcactc tagaatgcac aatggtttag tcactaaaaa attcaaatgg
241 gatcttgaag aatgtatgca aatccagggt gcagtgaaga tgagctgaga tgctgtgcaa
301 ctgtttaagg gttcctggca ctgcatctct tggccactag ctgaatcttg acatggaagg
361 ttttagctaa tgccaagtgg agatgcagat aatgctaagt tgacttaggg gctgtgcaca
421 ggaactaaaa ggcgggaaag tactaaatat tgctgagagc atccacccca ggaaggacct
481 taccttccag gagctccaa
//