LOCUS       AI085219                 511 bp    mRNA    linear   EST 05-JAN-2011
DEFINITION  oy16d08.s1 Soares_senescent_fibroblasts_NbHSF Homo sapiens cDNA
            clone IMAGE:1665999 3' similar to gb:V00572 PHOSPHOGLYCERATE KINASE
            1 (HUMAN), mRNA sequence.
ACCESSION   AI085219
VERSION     AI085219.1
DBLINK      BioSample: SAMN00155034
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 511)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1806   Std Error: 0.00
            Seq primer: -40m13 fwd. ET from Amersham
            High quality sequence stop: 312.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1665999"
                     /tissue_type="senescent fibroblast"
                     /clone_lib="SAMN00155034
                     Soares_senescent_fibroblasts_NbHSF"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker V_TYPE: phagemid; Site_1: Not I; Site_2: Eco
                     RI; 1st strand cDNA was primed with a Not I - oligo(dT)
                     primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo."
BASE COUNT          161 a          102 c          104 g          143 t
ORIGIN      
        1 tttttcacgg tttcaatgga cacttttatt gtttacttaa tggatcatca attttgtctc
       61 actacctaca aatggaattt catcttgttt ccatgctgag tagtgaaaca gtgacaaagc
      121 taatcataat aacctacatc aaaagagaac taagctaaca ctgctcactt tctttttaac
      181 aggcaaaata taaatatatg cactctagaa tgcacaatgg tttagtcact aaaaaattca
      241 aatgggatct tgaagaatgt atgcaaatcc agggtgcagt gaagatgagc tgagatgctg
      301 tgcaactgtt taagggttcc tggcactgca tctcttggcc actagctgaa tcttgacatg
      361 gaaggtttta gctaatgcca agtggagatg cagnaaatgc taagttgact tagtggctgt
      421 cacaggaacc taaagacagg gaagtactaa aattgctgag agcatccacc caggagggct
      481 ttccttccag agccccaact gggcacaacc c
//