LOCUS AI085219 511 bp mRNA linear EST 05-JAN-2011 DEFINITION oy16d08.s1 Soares_senescent_fibroblasts_NbHSF Homo sapiens cDNA clone IMAGE:1665999 3' similar to gb:V00572 PHOSPHOGLYCERATE KINASE 1 (HUMAN), mRNA sequence. ACCESSION AI085219 VERSION AI085219.1 DBLINK BioSample: SAMN00155034 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 511) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 1806 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 312. FEATURES Location/Qualifiers source 1..511 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1665999" /tissue_type="senescent fibroblast" /clone_lib="SAMN00155034 Soares_senescent_fibroblasts_NbHSF" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker V_TYPE: phagemid; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 161 a 102 c 104 g 143 t ORIGIN 1 tttttcacgg tttcaatgga cacttttatt gtttacttaa tggatcatca attttgtctc 61 actacctaca aatggaattt catcttgttt ccatgctgag tagtgaaaca gtgacaaagc 121 taatcataat aacctacatc aaaagagaac taagctaaca ctgctcactt tctttttaac 181 aggcaaaata taaatatatg cactctagaa tgcacaatgg tttagtcact aaaaaattca 241 aatgggatct tgaagaatgt atgcaaatcc agggtgcagt gaagatgagc tgagatgctg 301 tgcaactgtt taagggttcc tggcactgca tctcttggcc actagctgaa tcttgacatg 361 gaaggtttta gctaatgcca agtggagatg cagnaaatgc taagttgact tagtggctgt 421 cacaggaacc taaagacagg gaagtactaa aattgctgag agcatccacc caggagggct 481 ttccttccag agccccaact gggcacaacc c //