LOCUS AI080306 712 bp mRNA linear EST 08-JAN-2011 DEFINITION oz51h04.x1 Soares_senescent_fibroblasts_NbHSF Homo sapiens cDNA clone IMAGE:1678903 3' similar to gb:V00572 PHOSPHOGLYCERATE KINASE 1 (HUMAN), mRNA sequence. ACCESSION AI080306 VERSION AI080306.1 DBLINK BioSample: SAMN00155034 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 712) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 1838 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 352. FEATURES Location/Qualifiers source 1..712 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1678903" /tissue_type="senescent fibroblast" /clone_lib="SAMN00155034 Soares_senescent_fibroblasts_NbHSF" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker V_TYPE: phagemid; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 210 a 166 c 141 g 194 t ORIGIN 1 tttttttttt caatggacac ttttattgtt tacttaatgg atcatcaatt ttgtctcact 61 acctacaaat ggaatttcat cttgtttcca tgctgagtag tgaaacagtg acaaagctaa 121 tcataataac ctacatcaaa agagaactaa gctaacactg ctcactttct ttttaacagg 181 caaaatataa atatatgcac tctagaatgc acaatggttt agtcactaaa aaattcaaat 241 gggatcttga agaatgtatg caaatccagg gtgcagtgaa gatgagctga gatgctgtgc 301 aactgtttaa gggttcctgg cactgcatct cttggccact agctgaatct tgacatggaa 361 ggttttagct aatgccaagt ggagatgcag aaaatgctaa gttgacttag gggctgtgca 421 caggaactaa aaggcaggaa agtactaaat attgctgaga gcatccaccc caggaaggac 481 tttaccttcc aggagctcca aactggcacc acccccagtg ctcacatggc tgactttatc 541 ctccgtgttc catttggcac agcaagtggc agtgtctcca ccacctatga tggtgatgca 601 gccccctaga atggctttca ccacctcatc catgaaagct tggttccccg ggcaaagctt 661 ccattcaaat cccccacgga ccatccacac aatttgttan cccgatgaca gc //