LOCUS AI078722 471 bp mRNA linear EST 07-JAN-2011 DEFINITION oy12e04.s1 Soares_senescent_fibroblasts_NbHSF Homo sapiens cDNA clone IMAGE:1665630 3' similar to gb:V00572 PHOSPHOGLYCERATE KINASE 1 (HUMAN), mRNA sequence. ACCESSION AI078722 VERSION AI078722.1 DBLINK BioSample: SAMN00155034 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 471) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Trace considered overall poor quality Insert Length: 652 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham Sequence considered overall poor quality. FEATURES Location/Qualifiers source 1..471 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1665630" /tissue_type="senescent fibroblast" /clone_lib="SAMN00155034 Soares_senescent_fibroblasts_NbHSF" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker V_TYPE: phagemid; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 156 a 88 c 98 g 129 t ORIGIN 1 atggacactt ttattgttta cttaatggat catcaatttt gtctcactac ctacaaatgg 61 aatttcatct tgtttccatg ctcgagtagt gaaacagtga caaagctaat cataataacc 121 tacatcaaaa gagaactaag ctaacactgc tcactttctt tttaacaggc aaaatataaa 181 tatatgcact ctagaatgca caatggttta gtcactaaaa aattcaaatg ggatcttgaa 241 gaatgtatgc aaatccaggg tgcagtgaag atgagctgag atgctgtcgc aactcgttta 301 agggttcctg gcactgcatc tcttggccac tagctgaatc ttgacatgga aggttttagc 361 taatgccaag tggagatgca gaaaatgcta agttgactta ggggctgtgc acaggaacta 421 aaaggcagga aagtactaaa tattgctgag agcatccacc ccaggaagga c //