LOCUS       AI078722                 471 bp    mRNA    linear   EST 07-JAN-2011
DEFINITION  oy12e04.s1 Soares_senescent_fibroblasts_NbHSF Homo sapiens cDNA
            clone IMAGE:1665630 3' similar to gb:V00572 PHOSPHOGLYCERATE KINASE
            1 (HUMAN), mRNA sequence.
ACCESSION   AI078722
VERSION     AI078722.1
DBLINK      BioSample: SAMN00155034
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 471)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Trace considered overall poor quality
            Insert Length: 652   Std Error: 0.00
            Seq primer: -40m13 fwd. ET from Amersham
            Sequence considered overall poor quality.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1665630"
                     /tissue_type="senescent fibroblast"
                     /clone_lib="SAMN00155034
                     Soares_senescent_fibroblasts_NbHSF"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker V_TYPE: phagemid; Site_1: Not I; Site_2: Eco
                     RI; 1st strand cDNA was primed with a Not I - oligo(dT)
                     primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo."
BASE COUNT          156 a           88 c           98 g          129 t
ORIGIN      
        1 atggacactt ttattgttta cttaatggat catcaatttt gtctcactac ctacaaatgg
       61 aatttcatct tgtttccatg ctcgagtagt gaaacagtga caaagctaat cataataacc
      121 tacatcaaaa gagaactaag ctaacactgc tcactttctt tttaacaggc aaaatataaa
      181 tatatgcact ctagaatgca caatggttta gtcactaaaa aattcaaatg ggatcttgaa
      241 gaatgtatgc aaatccaggg tgcagtgaag atgagctgag atgctgtcgc aactcgttta
      301 agggttcctg gcactgcatc tcttggccac tagctgaatc ttgacatgga aggttttagc
      361 taatgccaag tggagatgca gaaaatgcta agttgactta ggggctgtgc acaggaacta
      421 aaaggcagga aagtactaaa tattgctgag agcatccacc ccaggaagga c
//