LOCUS       AF367252                1799 bp    DNA     linear   PLN 12-FEB-2004
DEFINITION  Trichosanthes kirilowii trichosanthin precursor (TCS) gene,
            complete cds.
ACCESSION   AF367252
VERSION     AF367252.1
KEYWORDS    .
SOURCE      Trichosanthes kirilowii
  ORGANISM  Trichosanthes kirilowii
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
            Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae;
            Sicyoeae; Trichosanthes.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Yuan,H., Wang,L., Wang,Y., An,C. and Chen,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2001) National Laboratory of Protein Engineering
            and Plant Genetic Engineering, College of Life Sciences, Peking
            University, Beijing 100871, China
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Trichosanthes kirilowii"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:3677"
                     /clone="type2"
     gene            1..>1799
                     /gene="TCS"
     regulatory      1..904
                     /regulatory_class="promoter"
                     /gene="TCS"
     misc_feature    903
                     /gene="TCS"
                     /note="alternative weaker transcriptional start site"
     mRNA            905..>1799
                     /gene="TCS"
                     /product="trichosanthin precursor"
     5'UTR           905..929
                     /gene="TCS"
     misc_feature    906
                     /gene="TCS"
                     /note="alternative weaker transcription start site"
     CDS             930..1799
                     /gene="TCS"
                     /function="inactivates eukaryotic 60S ribosomal subunits"
                     /note="preproprotein"
                     /codon_start=1
                     /product="trichosanthin precursor"
                     /protein_id="AAK52960.1"
                     /translation="MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNL
                     RKALPNERKLYDIPLLRSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDT
                     SYFFNEASATEAAKYVFKDSMRKITLPYSGNYERLQTAAGKIRENIPLGLPALDSAIT
                     TLFYYNANSAASALMVLIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSAL
                     SKQIQIASTNNGQFESPVVLINAQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDDV
                     PMTQSFGCGSYAI"
     sig_peptide     930..998
                     /gene="TCS"
     misc_feature    999..1796
                     /gene="TCS"
                     /note="encodes trichosanthin proprotein"
     mat_peptide     999..1739
                     /gene="TCS"
                     /product="trichosanthin"
BASE COUNT          612 a          331 c          295 g          561 t
ORIGIN      
        1 ataaaatctc aagttccttt cacacaaatt tcaataaaat ctttcgttgc tttcacaaag
       61 atttgaagac gtgattttcg tcctagcgtt taacagaatt ctacacgaaa agcggaccca
      121 aacatagtaa tttgccttct ctcctaggaa gtaagtttga accatcttca tttgtatgta
      181 atttaatgat tacaaaaaag tagataaaac tagaatgttg agcttttatt atatcccaaa
      241 gtgagcatag ctaaacggca attgacatat atctttcacc acgaggttag aggtttgaat
      301 ctcccattct caaatgttgt ttaattaaaa aaaaagtttt tattatacaa aatgcatgtt
      361 tgaaaatata ataagggtaa tataatataa gtcccatttg ataacgattt tgatctaatt
      421 tgataaccat ttagtttttg aaaattaagc ctataaatac tacttctatc catgggtttc
      481 cttattttgt tatctatttt ttacatatgt tttcaaaaac caagtcaaga tttgaaaact
      541 aaaaaaatta gttttcaaaa gcctattttt gtttttgaaa tttggctaag aattcaaata
      601 ttttctgaat aaatataaaa ttcattgtag agaaatgatg agaaaacaag tacaattttc
      661 aaaaacaaaa aacaaaaaac aaatggttta tcaaagaaga cccgaattta ttatcaaaag
      721 cgaaagttaa taatatctaa aaaaaaacta ttaccttata agaagctatt acctagatgg
      781 cataagatca tacttttatt tttgatttag actagaaata cactaatata ttgtggacat
      841 agaccagaca acgattagat ggaatacctc tctataaaaa ccacagcttg agatgctcca
      901 atggcatcca aattcctcaa gtcaaaaaga tgatcagatt cttagtcctc tctttgctaa
      961 ttctcaccct cttcctaaca actcctgctg tggagggcga tgttagcttc cgtttatcag
     1021 gtgcaacaag cagttcctat ggagttttca tttcaaatct gagaaaagct cttccaaatg
     1081 aaaggaaact gtacgatatc cctctgttac gttccagtct tccaggttct caacgctacg
     1141 cattgatcca tctcacaaat tacgccgatg aaaccatttc agtggccata gacgtaacga
     1201 acgtctatat tatgggatat cgcgctggcg atacatccta ttttttcaac gaggcttctg
     1261 caacagaagc tgcaaaatat gtattcaaag actctatgcg aaaaattacg cttccatatt
     1321 ctggcaatta cgaaaggctt caaactgctg caggcaaaat aagggaaaat attccgcttg
     1381 gactcccagc tttggacagt gccattacca ctttgtttta ctacaacgcc aattctgctg
     1441 cgtcggcact tatggtactc attcagtcga cgtctgaggc tgcgaggtat aaatttattg
     1501 agcaacaaat tgggaagcgt gttgacaaaa ccttcctacc aagtttagca attataagtt
     1561 tggaaaatag ttggtctgct ctctccaagc aaattcagat agcgagtact aataatggac
     1621 agtttgaaag tcctgttgtg cttataaatg ctcaaaacca acgagtcacg ataaccaatg
     1681 ttgatgctgg agttgtaacc tccaacatcg cgttgctgct gaatagaaac aatatggcag
     1741 ccatggatga cgatgttcct atgacacaga gctttggatg tggaagttat gctatttag
//