LOCUS       AF175265                2726 bp    mRNA    linear   HUM 22-DEC-2000
DEFINITION  Homo sapiens vacuolar sorting protein 35 (VPS35) mRNA, complete
            cds.
ACCESSION   AF175265
VERSION     AF175265.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Haft,C.R., de la Luz Sierra,M., Bafford,R., Lesniak,M.A., Barr,V.A.
            and Taylor,S.I.
  TITLE     Human orthologs of yeast vacuolar protein sorting proteins Vps26,
            29, and 35: assembly into multimeric complexes
  JOURNAL   Mol. Biol. Cell 11 (12), 4105-4116 (2000)
   PUBMED   11102511
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Renfrew Haft,C., Sierra,L. and Taylor,S.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-1999) Diabetes Branch, National Institutes of
            Health, 10 Center Dr., MSC-1770, Bethesda, MD 20892, USA
FEATURES             Location/Qualifiers
     source          1..2726
                     /db_xref="H-InvDB:HIT000072619"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /cell_line="HCC"
                     /cell_type="carcinoma"
                     /tissue_type="colon"
     gene            1..2726
                     /gene="VPS35"
     CDS             58..2448
                     /gene="VPS35"
                     /note="retromer protein; similar to MEM3"
                     /codon_start=1
                     /product="vacuolar sorting protein 35"
                     /protein_id="AAF89953.1"
                     /db_xref="GI:9622850"
                     /translation="MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALK
                     HASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAG
                     NIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNI
                     LPDEGEPPDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRI
                     LVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHL
                     QTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVAT
                     VIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSS
                     AVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTE
                     IVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYL
                     ILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQ
                     TISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQ
                     LAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRN
                     TDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTI
                     QVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL"
BASE COUNT          823 a          545 c          599 g          759 t
ORIGIN      
        1 aggaattcgg cacgaggcgg ggcgggtgct gcttgctgca ggctctgggg agtcgccatg
       61 cctacaacac agcagtcccc tcaggatgag caggaaaagc tcttggatga agccatacag
      121 gctgtgaagg tccagtcatt ccaaatgaag agatgcctgg acaaaaacaa gcttatggat
      181 gctctaaaac atgcttctaa tatgcttggt gaactccgga cttctatgtt atcaccaaag
      241 agttactatg aactttatat ggccatttct gatgaactgc actacttgga ggtctacctg
      301 acagatgagt ttgctaaagg aaggaaagtg gcagatctct acgaacttgt acagtatgct
      361 ggaaacatta tcccaaggct ttaccttttg atcacagttg gagttgtata tgtcaagtca
      421 tttcctcagt ccaggaagga tattttgaaa gatttggtag aaatgtgccg tggtgtgcaa
      481 catcccttga ggggtctgtt tcttcgaaat taccttcttc agtgtaccag aaatatctta
      541 cctgatgaag gagagccacc agatgaagaa acaactggtg acatcagtga ttccatggat
      601 tttgtactgc tcaactttgc agaaatgaac aagctctggg tgcgaatgca gcatcaggga
      661 catagccgag atagagaaaa aagagaacga gaaagacaag aactgagaat tttagtggga
      721 acaaatttgg tgcgcctcag tcagttggaa ggtgtaaatg tggaacgtta caaacagatt
      781 gttttgactg gcatattgga gcaagttgta aactgtaggg atgctttggc tcaagaatat
      841 ctcatggagt gtattattca ggttttccct gatgaatttc acctccagac tttgaatcct
      901 tttcttcggg cctgtgctga gttacaccag aatgtaaatg tgaagaacat aatcattgct
      961 ttaattgata gattagcttt atttgctcac cgtgaagatg gacctggaat cccagcggat
     1021 attaaacttt ttgatatatt ttcacagcag gtggctacag tgatacagtc tagacaagac
     1081 atgccttcag aggatgttgt atctttacaa gtctctctga ttaatcttgc catgaaatgt
     1141 taccctgatc gtgtggacta tgttgataaa gttctagaaa caacagtgga gatattcaat
     1201 aagctcaacc ttgaacatat tgctaccagt agtgcagttt caaaggaact caccagactt
     1261 ttgaaaatac cagttgacac ttacaacaat attttaacag tcttgaaatt aaaacatttt
     1321 cacccactct ttgagtactt tgactacgag tccagaaaga gcatgagttg ttatgtgctt
     1381 agtaatgttc tggattataa cacagaaatt gtctctcaag accaggtgga ttccataatg
     1441 aatttggtat ccacgttgat tcaagatcag ccagatcaac ctgtagaaga ccctgatcca
     1501 gaagattttg ctgatgagca gagccttgtg ggccgcttca ttcatctgct gcgctctgag
     1561 gaccctgacc agcagtactt gattttgaac acagcacgaa aacattttgg agctggtgga
     1621 aatcagcgga ttcgcttcac actgccacct ttggtatttg cagcttacca gctggctttt
     1681 cgatataaag agaattctaa agtggatgac aaatgggaaa agaaatgcca gaagattttt
     1741 tcatttgccc accagactat cagtgctttg atcaaagcag agctggcaga attgccctta
     1801 agactttttc ttcaaggagc actagctgct ggggaaattg gttttgaaaa tcatgagaca
     1861 gtcgcatatg aattcatgtc ccaggcattt tctctgtatg aagatgaaat cagcgattcc
     1921 aaagcacagc tagctgccat caccttgatc attggcactt ttgaaaggat gaagtgcttc
     1981 agtgaagaga atcatgaacc tctgaggact cagtgtgccc ttgctgcatc caaacttcta
     2041 aagaaacctg atcagggccg agctgtgagc acctgtgcac atctcttctg gtctggcaga
     2101 aacacggaca aaaatgggga ggagcttcac ggaggcaaga gggtaatgga gtgcctaaaa
     2161 aaagctctaa aaatagcaaa tcagtgcatg gacccctctc tacaagtgca gctttttata
     2221 gaaattctga acagatatat ctatttttat gaaaaggaaa atgatgcggt aacaattcag
     2281 gttttaaacc agcttatcca aaagattcga gaagacctcc cgaatcttga atccagtgaa
     2341 gaaacagagc agattaacaa acattttcat aacacactgg agcatttgcg cttgcggcgg
     2401 gaatcaccag aatccgaggg gccaatttat gaaggtctca tcctttaaaa aggaaatagc
     2461 tcaccatact cctttccatg tacatccagt gagggtttta ttacgctagg tttcccttcc
     2521 atagattgtg cctttcagaa atgctgaggt aggtttccca tttcttacct gtgatgtgtt
     2581 ttacccagca cctccggaca ctcaccttca ggaccttaat aaaattattc acttggtaag
     2641 tgttcaagtc tttctgatca ccccaagtag catgactgat ctgcaattta aaattcctgt
     2701 gatctgtaaa aaaaaaaaaa aaaaaa
//