LOCUS AF060183 4560 bp DNA linear BCT 06-NOV-1998
DEFINITION Mycobacterium avium putative glycosyltransferase,
rhamnosyltransferase (rtfA), and putative methyltransferase genes,
complete cds.
ACCESSION AF060183
VERSION AF060183.1
KEYWORDS .
SOURCE Mycobacterium avium
ORGANISM Mycobacterium avium
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium avium complex (MAC).
REFERENCE 1 (bases 1 to 4560)
AUTHORS Eckstein,T.M., Silbaq,F.S., Chatterjee,D., Kelly,N.J., Brennan,P.J.
and Belisle,J.T.
TITLE Identification and recombinant expression of a Mycobacterium avium
rhamnosyltransferase gene (rtfA) involved in glycopeptidolipid
biosynthesis
JOURNAL J. Bacteriol. 180 (21), 5567-5573 (1998)
PUBMED 9791104
REFERENCE 2 (bases 1 to 4560)
AUTHORS Eckstein,T.M., Silbaq,F.S., Inamine,I.M. and Belisle,J.T.
TITLE Direct Submission
JOURNAL Submitted (16-APR-1998) Microbiology, Colorado State University,
Fort Collins, CO 80523, USA
FEATURES Location/Qualifiers
source 1..4560
/organism="Mycobacterium avium"
/mol_type="genomic DNA"
/strain="724"
/db_xref="taxon:1764"
CDS 361..1638
/note="ORF1; similar to MTCY19G5_2 and MTCY19G5_4c of
Mycobacterium tuberculosis, and to U00023_2c of
Mycobacterium leprae"
/codon_start=1
/transl_table=11
/product="putative glycosyltransferase"
/protein_id="AAC71701.1"
/translation="MKCVLASYGTRGDIEPSVVVARELQRRGHDMIMAVPPDSVSFTE
AAGLTAVPYGLDSQAWLDVYRNFWTSFFRGFWKMGEMRGMWREMWDLSDQCWAQMNTT
LLSLADGADLLFAGQSYQEPAANVAEYYDLPLATLHHVPMRPNGQVVSVLPAPLARSA
MTAFDWFCWRLNKKVEDAQRRELGLPKATGPSPRRIAERGSLEIQAYDEVCFPGLAEE
WAKWDGQRPFVGTLTMELTTEADDEVMSWIRGGTPPICFGFGSMPVESPADTVDMIGT
ACAQLGERALLCAGRSDFSGVPAPDHVKVVGPVNYATIFPACRAVVHHGGSGTTAASL
RAGVPTLILSMDANQTIWGAQLKRLKVGATRRFSATDRDSLVQDLRKILAPDYARRAR
EIAARMTKPADSVMKAADVVEDFVQSRSRTGRS"
gene 2042..3328
/gene="rtfA"
CDS 2042..3328
/gene="rtfA"
/note="ORF2; similar to gene product of ORF1"
/codon_start=1
/transl_table=11
/product="rhamnosyltransferase"
/protein_id="AAC71702.1"
/translation="MKFAVASYGTRGDIEPCLAIGRELMRRGHSVRMAVPPNLVDLAE
AAGLSAIAYGPDMHDFWSDEFIRDFFKNFLRGPVTLIREAWEPVLKNWQEMSSALMSV
GAGADLLFTGQLYQDLAINVADYYGIPMATLHYIPMRPNGQLLPRVPGPLVRTGMAVY
DWMCWRMNKKAEDRQRRELGLPTATVASPRRIAERGSLEIQAYHEVCFPGLAEEWAKW
GGQRPFVGSLTMELPTDFDDEVLSWIGQGTPPICFGFGSMPVAESPAETIEIIGTACA
ELGERALICSGWSDFSNVPHADHVKVVGVVNYTKIFPACRAVVHHGGSGTTAAGLRAG
IPSLILWTAGDQPLWGSRIKQLKVGTSRRFSATTPETLVADLRKILAPEYLTRARELS
SRMSKPAESAGRAVDLLEEFARSGRCIPRAEAQRSR"
CDS 3430..4230
/note="ORF3; similar to mycinaminIII O-methyltransferase
of Micromonospora griseorubida"
/codon_start=1
/transl_table=11
/product="putative methyltransferase"
/protein_id="AAC71703.1"
/translation="MTDHDTRFAYLDLLRRDLTRYGSDELVPVGLYRLGRPLFSTRNL
MLVRKRPFNKRARDLGLDWPADALTMIGMKRLTSLQHCVETVLEEDVPGDLVECGVWR
GGASILMRAVLAAYGDEKRCVWLCDSFAGVPPPDTVNYKADKGIRLHRHARILGVPLE
NVKANFERYGLLDDQVRFVPGWFKDTLKDAPIDRISVLRLDGDLYESTIQALDALYPR
LSPGGFCIVDDYHAIKACAQAVTDYRTQHGVTAEIVEIDGTGVLWRKP"
BASE COUNT 783 a 1453 c 1536 g 788 t
ORIGIN
1 aggatgcgcc gatcgaacgg atcgccgtgc tgcgcctcga cggcgacctg tatgaatcga
61 cgatccaggc cctggacgcg ctctacccgc ggctctcggc gggcggcatc tgcatcatcg
121 acgactacca cgccatcgac gcctgccggc aggcggtgac cgactaccgt tccgaacacg
181 gcgtgaccgc gccgatcgag gagatcgacg gcaccggcgt gctgtggcgc aagtcctgag
241 ggcaaccttt atcgggccgc gtcggcgcag aaccgtaaac cggtgctgtg cggattcgtc
301 gatacctaga gtggtcggtg catcaccgtc gaggacgcaa ccgaagggct tggattcgcg
361 atgaaatgtg tgctggccag ctacgggacg cgcggggaca tcgagccatc cgtggtggtc
421 gcccgcgaac tgcagcgccg gggccacgac atgatcatgg ccgtgccgcc cgactcggtg
481 agcttcaccg aggcggccgg gttgacggcc gtcccctacg ggctggactc gcaggcctgg
541 ctcgacgtgt accgcaactt ctggacgtcg ttcttccggg ggttctggaa gatgggggag
601 atgcgcggca tgtggcgcga gatgtgggat ctcagcgacc agtgctgggc gcagatgaac
661 accacgctgc tgtcgctggc cgacggagcc gacctgctgt tcgccgggca gagctaccag
721 gagcccgccg ccaacgtcgc cgagtattac gaccttccgc tggccaccct gcaccacgtg
781 ccgatgcggc ccaacggcca ggtcgtttcg gtgctgccgg cgccgctggc ccgctcggcg
841 atgacggcgt tcgactggtt ctgctggcgg ctgaacaaga aggtcgagga cgcccagcgc
901 cgcgagctgg gcctgcccaa agcgaccgga ccgtcgccgc ggcggatcgc cgagcgcggc
961 tcgctggaaa tccaggccta cgacgaggtc tgcttccccg gcctggccga ggaatgggcg
1021 aaatgggatg gccaacggcc cttcgtcggc acgctgacga tggagttgac caccgaggcc
1081 gacgacgagg tgatgtcctg gatccgcggc ggaacgccgc cgatctgctt cgggttcggc
1141 agcatgcccg tcgaatcgcc ggccgacacg gtcgacatga tcggcacggc ctgcgcccag
1201 ctgggcgagc gcgcgctgct gtgcgccggc cgcagtgact tcagcggcgt cccggcgccc
1261 gatcacgtca aggtggtggg cccggtgaac tacgccacca ttttcccggc ctgccgcgcg
1321 gtcgtgcacc acggcggctc gggcaccacg gccgcgagcc tgcgcgccgg cgtgcccacc
1381 ttgatcctgt ccatggacgc caaccagacc atctggggcg cacagctcaa acggctgaaa
1441 gtcggtgcca cacggcgttt ttcggcaacc gaccgcgact cgctggtgca ggatctgcgc
1501 aagatcctgg ccccggatta cgctcggcgc gcccgcgaga tcgccgcccg gatgaccaag
1561 cccgccgaca gcgtcatgaa ggccgccgat gtcgtggagg acttcgtcca atcccgcagc
1621 cgcaccggtc gcagctaacc tggcgcatgg ccgctgcgct gggcaaacgg ccaggattgc
1681 taccccgcag gtgcccgcac acccgccggt tgacttcccg ttttccaaga cacccgatcg
1741 atcgtctggc acactgacgt aggatcaggc gacgacgctt gggtacagcc acaccgccca
1801 agggtcgtct tgtttctacg gtcgggggga aggccgatga ggttggctct gtcgcactcg
1861 gctactccga tgcgccgtga tcttcccgct gggtggggtg tttccaaatc cgttgcgaaa
1921 caactacaac caatgacttc gaccgggccc cggggccgat ggcgggccgc agatcaccga
1981 gaggaaacgc cgccgccaaa gttgcccatg ctgtcggaga ttgattaagg taataatcgc
2041 gatgaaattt gctgtggcaa gttatgggac gcgtggcgat atcgagcctt gcctcgccat
2101 tggtcgtgaa ctgatgcgca gagggcattc ggtacgtatg gccgtcccgc ccaatctggt
2161 cgatttagcc gaagccgccg ggctgtcggc gatcgcgtat gggccggata tgcacgactt
2221 ttggagcgac gagttcatcc gggacttctt caaaaacttc ctgcgcggcc cggtcacatt
2281 gatccgcgaa gcctgggaac cggtattgaa gaactggcag gaaatgagct cggcgttgat
2341 gtcggtgggc gccggagctg atttgctgtt caccggccag ctttaccagg acctggccat
2401 caacgtcgcc gactattacg gcatcccgat ggccacgctg cactacattc cgatgcgccc
2461 caacggtcag ctgctgccgc gggtgccggg accgttggtc cgcaccggga tggcggtcta
2521 cgactggatg tgctggcgga tgaacaagaa ggccgaggac aggcagcgcc gcgaactcgg
2581 cctaccgacc gccacggtgg cctcaccgcg ccggatcgcc gagcgtgggt cgctggaaat
2641 ccaggcctac cacgaggttt gctttcccgg gttggccgag gaatgggcga aatggggcgg
2701 tcagcggccg ttcgtgggct cgctgaccat ggagctgccc accgacttcg atgacgaggt
2761 gttgtcctgg atcggccagg gaacgccgcc gatctgcttc ggcttcggca gcatgccggt
2821 ggccgaatcg cctgccgaga cgatcgaaat catcggcacg gcatgcgccg aattggggga
2881 gcgggcgttg atctgctcgg gctggagtga cttcagcaat gtcccgcacg ccgaccacgt
2941 caaggtggtc ggcgtggtca actacaccaa gatcttcccg gcctgccgcg cggtcgtgca
3001 ccacggcggc tcggggacca ccgccgccgg cctgcgggcg ggaattccca gcctgatcct
3061 gtggactgcg ggggatcagc cgctgtgggg gtcacgcatc aagcagctga aggtgggcac
3121 ctcgcggcgg ttctcggcca ccacgcccga aacgttggtg gccgacctgc ggaagatctt
3181 ggcgcccgaa tacctcaccc gcgcgcgcga actgtcctcg cggatgagca agcccgccga
3241 gagcgcgggc cgggcggtcg acctgctgga ggaattcgct cgctcgggcc gctgcattcc
3301 gcgggccgaa gcgcagcgaa gtcgctgaga accgttgacc gataccgtgc gaaacgcatt
3361 gacggcgtcg atggacgtct aggctctacc ggtaatacag gcaagcggag taaggggcaa
3421 gttttggccg tgaccgatca cgacacgcgg tttgcatacc tcgacctgct ccggcgtgat
3481 ctgaccaggt atggcagcga cgagttggtg ccggtggggt tgtaccgcct ggggcgcccg
3541 ctgttcagca cccgcaactt gatgttggtg cgcaagcgtc cgttcaacaa acgagctcgt
3601 gatctaggcc tggattggcc ggcggacgcg ctgaccatga tcggcatgaa gcggctgacc
3661 agcttgcagc actgcgtgga aacggttctg gaagaagacg ttccgggcga tctggtcgag
3721 tgcggcgtgt ggcgtggcgg ggcgtcgatc ttgatgcgtg ccgtgctggc ggcttacggc
3781 gacgagaagc gctgcgtgtg gctgtgcgat tcctttgccg gagtgccgcc gccggacacc
3841 gtcaactaca aggcggacaa ggggattagg ctgcaccgtc acgcgcgcat cctgggtgtg
3901 ccgctggaga acgtcaaggc gaatttcgag cgctacgggc tgctcgacga ccaggtgcgt
3961 ttcgtgcccg gctggttcaa ggacacgctg aaagacgccc cgatcgatcg catctcggtg
4021 ttacggctcg acggcgatct ctacgaatcc accatccagg cgctcgacgc gctgtacccc
4081 cgactgtccc ccggcggctt ctgcatcgtc gacgactacc acgccatcaa ggcgtgcgcg
4141 caggccgtga cggactaccg cacccagcac ggtgtcaccg cggagatcgt agagatcgac
4201 ggcaccggtg tgctgtggcg caagccctga gagatcgctg aaaccctcgg tgttcgaaac
4261 gatttgccct ccggtcgggg atgggcgcaa gagcgtcacg tcgaaccctt cgcgtatttt
4321 gcggaccggg tgcacgccga tgttatggtt gccgccgtgg ctacagggct gacccccggc
4381 acgcgcctgc ggatgtggtg ggtgcgcacc gaatactggc tggcgcgcac actgctgccg
4441 gacgtctacg ccaacgatgc gctgatcacc ttcaacaact ttcatgcatt cctggacgat
4501 cccgaattcc agcgcgccta ccggcgcggc gcccgctcgc tgggcaagga ggattggtac
//