LOCUS       AB451439                 588 bp    mRNA    linear   HUM 02-SEP-2008
DEFINITION  Homo sapiens PDGFA mRNA for platelet-derived growth factor alpha
            isoform 2 preproprotein, partial cds, clone: FLJ08166AAAF.
ACCESSION   AB451439
VERSION     AB451439.1
KEYWORDS    Gateway cloning system.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Kawamura,Y., Nomura,N. and Goshima,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2008) to the DDBJ/EMBL/GenBank databases.
            Contact:Naoki Goshima
            Biomedicinal Information Research Center (BIRC), National
            Institute of Advanced Industrial Science and Technology (AIST);
            2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan
REFERENCE   2
  AUTHORS   Goshima,N., Kawamura,Y., Fukumoto,A., Miura,A., Honma,R.,
            Satoh,R., Wakamatsu,A., Yamamoto,J.-I., Kimura,K., Nishikawa,T.,
            Andoh,T., Iida,Y., Ishikawa,K., Ito,E., Kagawa,N., Kaminaga,C.,
            Kanehori,K., Kawakami,B., Kenmochi,K.-I., Kimura,R., Kobayashi,M.,
            Kuroita,T., Kuwayama,H., Maruyama,Y., Matsuo,K., Minami,K.,
            Mitsubori,M., Mori,M., Morishita,R., Murase,A., Nishikawa,A.,
            Nishikawa,S., Okamoto,T., Sakagami,N., Sakamoto,Y., Sasaki,Y.,
            Seki,T., Sono,S., Sugiyama,A., Sumiya,T., Takayama,T.,
            Takayama,Y., Takeda,H., Togashi,T., Yahata,K., Yamada,H.,
            Yanagisawa,Y., Endo,Y., Imamoto,F., Kisu,Y., Tanaka,S., Isogai,T.,
            Imai,J.-I., Watanabe,S. and Nomura,N.
  TITLE     Human Protein Factory: an infrastructure to convert the human
            transcriptome into the in vitro-expressed human proteome of
            versatile utility
  JOURNAL   Unpublished (2008)
COMMENT     This human Gateway entry clone was constructed by recombining an
            attB-attached open reading frame (ORF) fragment with attP
            sequences of the Gateway donor vector pDONR201. The ORF sequence
            in the entry clone is flanked with attL1 sequence (100 nt) -
            spacer nucleotides (TC) - Shine-Dalgano sequence (GAAGGAGATA) -
            spacer nucleotides (GA) - Kozak sequence (ACC) upstream of the
            translational initiation codon (ATG) and is also flanked with
            spacer nucleotides (TATG) - attL2 sequence (99 nt) downstream of
            the ORF. This is an F-type clone that deletes the stop codon for
            C-terminal tagged proteins. DNA sequences of the entire ORF and
            flanking regions were validated by sequencing. Clone structure of
            the ORF and flanking sequences is as follows:
            5'-caaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgatgagcaatgct
            tttttataatgccaactttgtacaaaaaagcaggct TC GAAGGAGATA GA ACC 5'ORF3'
            TATG acccagctttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaac
            gaacaggtcactatcagtcaaaataaaatcattattg-3' (5'ORF3'; ORF sequence.
            attL sequences are described in lower cases).
            Clone information: http://www.HGPD.jp/
            This clone was produced in the "Functional Analysis of Protein and
            Research Application" project supported by the New Energy and
            Industrial Technology Development Organization (NEDO), Japan
FEATURES             Location/Qualifiers
     source          1..588
                     /clone="FLJ08166AAAF"
                     /db_xref="H-InvDB:HIT000487652"
                     /db_xref="taxon:9606"
                     /mol_type="mRNA"
                     /note="Vector: pDONR201"
                     /organism="Homo sapiens"
     CDS             1..>588
                     /codon_start=1
                     /gene="PDGFA"
                     /product="platelet-derived growth factor alpha isoform 2
                     preproprotein"
                     /protein_id="BAG70253.1"
                     /transl_table=1
                     /translation="MRTLACLLLLGCGYLAHVLAEEAEIPREVIERLARSQIHSIRDL
                     QRLLEIDSVGSEDSLDTSLRAHGVHATKHVPEKRPLPIRRKRSIEEAVPAVCKTRTVI
                     YEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRK
                     KPKLKEVQVRLEEHLECACATTSLNPDYREEDTDVR"
BASE COUNT          133 a          173 c          182 g          100 t
ORIGIN      
        1 atgaggacct tggcttgcct gctgctcctc ggctgcggat acctcgccca tgttctggcc
       61 gaggaagccg agatcccccg cgaggtgatc gagaggctgg cccgcagtca gatccacagc
      121 atccgggacc tccagcgact cctggagata gactccgtag ggagtgagga ttctttggac
      181 accagcctga gagctcacgg ggtccatgcc actaagcatg tgcccgagaa gcggcccctg
      241 cccattcgga ggaagagaag catcgaggaa gctgtccccg ctgtctgcaa gaccaggacg
      301 gtcatttacg agattcctcg gagtcaggtc gaccccacgt ccgccaactt cctgatctgg
      361 cccccgtgcg tggaggtgaa acgctgcacc ggctgctgca acacgagcag tgtcaagtgc
      421 cagccctccc gcgtccacca ccgcagcgtc aaggtggcca aggtggaata cgtcaggaag
      481 aagccaaaat taaaagaagt ccaggtgagg ttagaggagc atttggagtg cgcctgcgcg
      541 accacaagcc tgaatccgga ttatcgggaa gaggacacgg atgtgagg
//