LOCUS AB267085 4411 bp DNA circular SYN 19-DEC-2007
DEFINITION Shuttle vector pNV18 DNA, complete sequence.
ACCESSION AB267085
VERSION AB267085.2
KEYWORDS .
SOURCE Shuttle vector pNV18
ORGANISM Shuttle vector pNV18
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4411)
AUTHORS Ishikawa,J. and Chiba,K.
TITLE Direct Submission
JOURNAL Submitted (27-JUL-2006) to the DDBJ/EMBL/GenBank databases.
Contact:Jun Ishikawa
National Institute of Infectious Diseases, Department of Bioactive
Molecules; 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan
URL :http://nocardia.nih.go.jp/
REFERENCE 2
AUTHORS Chiba,K., Hoshino,Y., Ishino,K., Kogure,T., Mikami,Y., Uehara,Y.
and Ishikawa,J.
TITLE Construction of a pair of practical Nocardia-Escherichia coli
shuttle vectors.
JOURNAL Jpn. J. Infect. Dis. 60, 45-47 (2007)
COMMENT
FEATURES Location/Qualifiers
source 1..4411
/db_xref="taxon:396412"
/mol_type="other DNA"
/organism="Shuttle vector pNV18"
misc_feature 1..2661
/note="derived from pK18"
CDS complement(1512..2306)
/codon_start=1
/gene="neo"
/product="aminoglycoside 3'-O-phosphotransferase"
/protein_id="BAF02355.1"
/transl_table=11
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 2662..4411
/note="derived from pAL5000"
CDS complement(2746..3093)
/codon_start=1
/gene="repB"
/product="replication protein RepB"
/protein_id="BAF02356.2"
/transl_table=11
/translation="MSDGYSDGYNRQPTVRKKRRVTAAEGARITGLSERHVVRLVAQE
RSEWLAEQAARRERIRAYHDDEGHSWPQTAKHFGLHLDTVKRLGYRARKERAAEQEAA
QKAHNEADNPPLF"
CDS complement(3090..4016)
/codon_start=1
/gene="repA"
/product="replication protein RepA"
/protein_id="BAF02357.1"
/transl_table=11
/translation="MSHVADEFEQLWLPYWPLASDDLLEGIYRQSRASALGRRYIEAN
PTALANLLVVDVDHPDAALRALSARGSHPLPNAIVGNRANGHAHAVWALNAPVPRTEY
ARRKPLAYMAACAEGLRRAVDGDRSYSGLMTKNPGHIAWETEWLHSDLYTLSHIEAEL
GANMPPPRWRQQTTYKAAPTPLGRNCALFDSVRLWAYRPALMRIYLPTRNVDGLGRAI
YAECHARNAEFPCNDVCPGPLPDSEVRAIANSIWRWITTKSRIWADGIVVYEATLSAR
QSAISRKGAAARTAASTVARRAKSASAMEALL"
CDS complement(join(4405..4411,1..242))
/codon_start=1
/gene="lacZ"
/product="beta-galactosidase alpha fragment"
/protein_id="BAF02358.1"
/transl_table=11
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDKLA"
BASE COUNT 852 a 1334 c 1297 g 928 t
ORIGIN
1 agcttatcgc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
61 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
121 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagcttgca
181 tgcctgcagg tcgactctag aggatccccg ggtaccgagc tcgaattcgt aatcatggtc
241 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
301 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
361 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
421 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
481 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
541 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
601 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
661 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
721 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
781 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
841 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
901 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
961 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
1021 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
1081 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
1141 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
1201 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
1261 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
1321 cttcacctag atccttttgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
1381 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
1441 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
1501 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
1561 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
1621 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
1681 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
1741 ccatgggtca cgacgagatc ctcgccgtcg ggcatccgcg ccttgagcct ggcgaacagt
1801 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
1861 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
1921 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
1981 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
2041 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
2101 cacgatagcc gcgctgcctc gtcttggagt tcattcaggg caccggacag gtcggtcttg
2161 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
2221 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
2281 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat
2341 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
2401 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
2461 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
2521 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
2581 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag
2641 tgcttgcggc agcgtgaagc tagcgtctac caggactttt acctgtccga cccgttgcaa
2701 cggaaccccc cacggaaccc ccgcgacacc cgctccccaa ttgcgttaga acagcggtgg
2761 attgtcggct tcgttgtggg ccttttgagc cgcttcctgt tctgccgcac gctctttcct
2821 cgcccgatag ccgagtcgct taacggtgtc cagatgcagc ccgaaatgtt tggccgtttg
2881 cggccaagag tggccctcgt cgtcgtgata ggcgcggatg cgttcgcggc gtgcagcctg
2941 ctcggcgagc cactcgctgc gttcctgcgc cacgagccgg acgacgtggc gttcggatag
3001 tccggtgatt cgagcgcctt cggcggcggt cacgcgccgc tttttgcgga cagtcggctg
3061 ccggttgtag ccgtcgctgt agccgtcgct catagcaatg cctccatggc tgacgcggac
3121 tttgcgcgcc gcgcaactgt gctcgccgcc gtgcgcgctg ctgcgccctt ccgcgagatg
3181 gccgactggc gcgcactgag tgtggcctcg tagaccacga tcccgtccgc ccaaatgcgc
3241 gacttggttg tgatccaacg ccaaatgctg ttggcgatgg cgcggacctc gctgtccggt
3301 agcggtccgg gacacacgtc gttgcacggg aactcggcgt ttcgcgcgtg gcactcggca
3361 tagatcgcgc ggccgagtcc gtccacgttc cgggtcggca ggtagatccg catgagggcg
3421 ggacgatagg cccacaacct gacggaatcg aacagtgcgc aattccgccc tagcggcgtc
3481 ggagccgctt tgtacgtggt ctgctgacgc cagcgcggcg gtggcatgtt cgcgccgagc
3541 tcggcctcga tgtggctgag tgtgtagaga tctgagtgga gccattccgt ttcccaggcg
3601 atgtggccgg ggtttttggt catgaggcct gagtaactgc ggtcgccgtc cacggcgcgc
3661 cgaaggcctt cggcgcacgc cgccatgtat gcgagcggct tacgccgcgc gtattcggtg
3721 cgtggaacag gggcgttgag tgcccacact gcgtgtgcgt ggccgttggc gcgattgccc
3781 acgatcgcgt tgggcagcgg atgggacccc cgggcgctga gcgctcggag cgctgcgtct
3841 ggatggtcta cgtccacgac cagcaggttt gccagcgctg ttgggttcgc ctcgatgtac
3901 cggcggccta gggccgacgc gcggctttgg cggtagatcc cctcgagcag atcgtcgctt
3961 gccagcggcc agtacggcag ccagagctgc tcaaattcgt cggcgacgtg gctcacgctt
4021 ggtagtagac cacgattaat caccggtgta tggtccgaca cgagctccaa gtcagatatt
4081 tcgctgaggg gccaccccac aactgcacac tcccccgctc tcccgtcgag ccctggtggt
4141 ggaacaccag cgacagccga gcacccccaa ccacctgtac caaccaggag gaacacatgc
4201 gtcgtttcga ggacgtttcc gggccgctga gagccgctgt ggcggccgta cacgccgcct
4261 tagacccgtt agaccccctg ccgcctgaat gcgcgggtac gagccacaca gcgcccgaac
4321 ttacggagct ggtgggctca cctggcttta tggcgtacga atcggctgtg tgcgacctgt
4381 tgggcgaggt gaggtacgcg ctactcacgc t
//