LOCUS AB262552 2531 bp DNA linear PLN 15-AUG-2009
DEFINITION Ligusticum sinense chloroplast trnK, matK genes for tRNA-Lysine,
maturase, partial and complete cds.
ACCESSION AB262552
VERSION AB262552.1
KEYWORDS .
SOURCE chloroplast Ligusticum sinense
ORGANISM Ligusticum sinense
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
Pentapetalae; asterids; campanulids; Apiales; Apiaceae; Apioideae;
Acronema clade; Ligusticum.
REFERENCE 1 (bases 1 to 2531)
AUTHORS Zhu,S., Fushimi,H. and Komatsu,K.
TITLE Direct Submission
JOURNAL Submitted (12-JUN-2006) to the DDBJ/EMBL/GenBank databases.
Contact:Katsuko Komatsu
Institute of Natural Medicine, University of Toyama, Division of
Pharmacognosy, Department of Medicinal Resources; Sugitani 2630,
Toyama, Toyama 930-0194, Japan
REFERENCE 2
AUTHORS Zhu,S., Fushimi,H., Han,G., Tsuchida,T., Uno,T., Takano,A. and
Komatsu,K.
TITLE Molecular Identification of "Chuanxiong" by Nucleotide Sequence and
Multiplex Single Base Extension Analysis on Chloroplast trnK Gene
JOURNAL Biol. Pharm. Bull. 30, 527-531 (2007)
COMMENT
FEATURES Location/Qualifiers
source 1..2531
/cultivar="Chuanxiong"
/db_xref="taxon:49555"
/mol_type="genomic DNA"
/note="synonym: Ligusticum chuanxiong"
/organelle="plastid:chloroplast"
/organism="Ligusticum sinense"
/PCR_primers="fwd_name: trnK3914F, fwd_seq:
TGGGTTGCTAACTCAATGG, rev_name: trnK2R, rev_seq:
AACTAGTCGGATGGAGTAG"
tRNA <1..19
/gene="trnK"
/note="5' exon of transfer RNA for Lysine"
/product="tRNA-Lysine"
intron 20..>2531
/gene="trnK"
/note="intron of trnK gene"
CDS 757..2274
/codon_start=1
/gene="matK"
/note="It is an open reading frame (ORF) located within
the intron of the transfer RNA gene for Lysine (trnK)"
/product="maturase"
/protein_id="BAF50736.1"
/transl_table=11
/translation="MEEFQRYLKLNISQQHYFLYPLIFQEYIYALAHDHGLNRNRSVL
LENAGYNKFSLLIVKRAIERMYQYEQNHLILFANDFTQNTFFGRNKNFNFQMISEGFA
VIVEIPFYLRLLFSIERKGIVKSHNLRSIHSIFPFLEDKFSHLIYVLEILIPYPVHLE
ILVQTLRYWVKDASSLHLLRFFLHEYRIWNTPNKASSCFSKRNQRFFFVLYNSHLCEY
ESIFVFLRNKSSHLCSTSSGTLLERIFFYGKLEHLGLVEAFAKAFQDSLWLFKDPLMH
YISYQGKSILASKGTPLLMKKWTYFFVNLWKCHFYLWSQPGRICINQLYNHSLALLGY
LSSARLNPSMVRSQMLENAFIIDNPINKFDTLVPIVPLIGSLAKARFCNVLGHPISKA
VWTDLSDSDIVVRFGRICRNISHYYSGSSQKKSLYRIKYILRLSCARTLARKHKSTVR
AFFKRSGSGLLEEFFTAEEQVLYLTFPRASSTSQRLYRRRIWYLDIICINDLASY"
BASE COUNT 798 a 402 c 461 g 870 t
ORIGIN
1 tagagtacac ggcttttaag tgcggctagg atcttttaca catttggatg aagcgaggga
61 ttcgtccata ccatcggtaa agtttggaag accacgactg atcctgaaag ggaatgaatg
121 gaaaaaatag catgtcggat caacgaagag ttctgagaat atttcattct ttccgaatcg
181 gtacaaaccg ttgtgttctt ttttgaaacg gaacaaaatg aattcaattt caagttgggt
241 cggatgaata aatggatata gagccctaat ggcttcaatt tatttattta ttgatatgat
301 tattgattat agggaaaaag caacgagctt ctgttcttaa tttgaacgat tacccgatct
361 aattagacgt taaaaatgta ttagtgcctg atacgggaag ggattatccc atgaacgaat
421 tctttatatt ttaatgaatc ctaactattg acattttcca ttatggaata gaaatgtgtg
481 tgtagaagaa acagtatatt gataaaaaaa ttccaaaatc aaaagagcga ttaggttgaa
541 aaaataaagg atttctaagt cttttgttat cctataacga acataaactt attcgtttaa
601 atggaaaaga gaggatagat aatccgttga taagtttacc tgtatccccg aggtatctat
661 tcgtttatta ctcgaatacc tcgttttgac tgtatcgcac tatgtttcat tgataacccc
721 caaaatcctc tacccttagt tcaactagaa tttcaaatgg aggaattcca aagatattta
781 aagctaaata tatctcaaca acactacttc ttatatccac ttatctttca ggagtatatt
841 tatgcacttg cgcatgatca tggtttaaat agaaatagat ccgttttgtt ggaaaatgca
901 ggttataata agttcagctt actaattgtg aaacgtgcaa ttgagcgaat gtatcagtat
961 gaacagaatc atttgattct ttttgctaat gattttaccc aaaatacctt ttttgggcgc
1021 aacaagaatt ttaattttca aatgatatca gaaggatttg cagtcattgt agaaattccg
1081 ttttatctcc gattattatt ttcgatagaa aggaaaggaa tagttaaatc tcataattta
1141 cgatcaattc attcaatatt ccctttttta gaggacaaat tttcacattt aatttatgtg
1201 ttagagatac taatacccta cccagtccat ctggaaatat tggttcaaac tcttcgctac
1261 tgggtaaaag acgcttcttc tttacattta ttaagattct ttctccacga gtatcgtatt
1321 tggaatactc caaataaagc cagttcttgt ttttcaaaaa gaaatcaaag gtttttcttc
1381 gtcctatata attctcatct atgtgaatac gaatccatct tcgtctttct tcgtaacaaa
1441 tcttctcatt tatgctcaac gtcttctgga acccttcttg aacgaatctt tttctatgga
1501 aaactagaac atcttggact tgtagaagct tttgctaagg cctttcagga cagtctatgg
1561 ttgtttaagg accctttgat gcattatatt agttatcaag gaaaatcaat tctcgcttca
1621 aaagggacgc cccttttgat gaaaaaatgg acatattttt ttgtcaattt atggaaatgt
1681 catttttacc tatggtctca gccgggacgg atctgtataa accaattata taatcattcc
1741 ctagctcttc tgggctatct atcaagtgcg cgactaaacc cttcaatggt acgcagtcaa
1801 atgctagaaa atgcatttat aattgataat cctattaata agttcgatac tcttgttcca
1861 attgttcctc taattggatc attggctaag gcgagatttt gtaacgtatt ggggcaccct
1921 attagtaagg cggtttggac tgatttatca gattctgata ttgttgtccg atttgggcgt
1981 atctgcagaa atatttctca ttattatagt ggatcctcac aaaaaaagag tttgtatcga
2041 ataaagtata tacttcgact gtcttgtgct agaactttgg ctcgtaaaca taaaagtact
2101 gtacgtgctt tttttaaaag atcgggctcg gggttattgg aagaattctt tacggcggaa
2161 gagcaagtcc tttatttgac ctttccaaga gcttcttcca cttcgcagag gttatataga
2221 aggcgtattt ggtatttgga tattatttgt atcaatgatt tggccagtta ttaattgaat
2281 gattcgttag gaaatcatgt aaattgtcac taaaaatgta ggtaatgaag ataaaaaatg
2341 aagataacaa tttttttttc tgaaatgttg atgtaggatg tagtaagggt taaatcaacc
2401 gagtattcaa cttttttaaa gttctaagta agaaaccggg ttttaaatgt atacataggg
2461 aaagccgtgt gcaatgaaaa atgcaagcac ggtttgggga ggggtgttta cctatttaac
2521 aggtaaatta t
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