LOCUS AB220413 1642 bp mRNA linear PRI 06-OCT-2006
DEFINITION Macaca fascicularis mRNA, clone QccE-14152: similar to Homo
sapiens epoxide hydrolase 1(EPHX1), NM_000120.2.
ACCESSION AB220413
VERSION AB220413.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 1642)
AUTHORS Hashimoto,K., Osada,N. and Kusuda,J.
TITLE Direct Submission
JOURNAL Submitted (04-JUL-2005) to the DDBJ/EMBL/GenBank databases.
Contact:Katsuyuki Hashimoto
National Institute of Biomedical Innovation, Division of
Biomedical Resources; 7-6-8, Saito-Asagi, Ibaraki, Osaka 567-0085,
Japan
REFERENCE 2
AUTHORS Kobayashi,M., Tanuma,R., Hirata,M., Osada,N., Kusuda,J., Sugano,S.
and Hashimoto,K.
TITLE Analysis of gene expression in cynomolgus monkey tissues by
macaque cDNA oligo-chips
JOURNAL Unpublished (2005)
COMMENT Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5' and 3' primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
oligo-capping method. Libraries were made from:
QccE: cerebellum cortex
QnpA: parietal lobe
QtrA: temporal lobe right
QflA: frontal lobe left
QmoA: medulla oblongata
QbsA(B): brain stem
QorA: occipital lobe right
QtsA: testis
Custom primers were used for 5' and 3'-end sequencing.
The full-insert sequencing was done by primer-walking method using
ABI DNA sequencer.
FEATURES Location/Qualifiers
source 1..1642
/clone="QccE-14152"
/clone_lib="macaque brain cDNA library QccE"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
/sex="male"
/tissue_type="cerebellum cortex"
CDS 49..1416
/codon_start=1
/note="similar to Homo sapiens epoxide hydrolase
1(EPHX1), NM_000120.2."
/product="hypothetical protein"
/protein_id="BAE72946.1"
/transl_table=1
/translation="MWLEILLTSVLGFAIYWFISRDKEETLPLEDGWWGPGTRPTGRE
DESIHPFKVETSDEEIHDLHQRIDKFRFTPPLEDSCFHYGFNSNYLKKVISYWRNEFN
WKKQVEILNRYPHFKTKIEGLDIHFIHVKPPQLPAGRTPKPLLMVHGWPGSFYEFYKI
IPLLTDPKNHGLSDEHVFEVICPSIPGYGFSEASSKKGFNSVATARIFYKLMLRLGFQ
EFYIQGGDWGALICTNMAQLVPSHVKGLHLNMALVLSNFSTLTLLLGRRFGRFLGYTE
RDVELLYPVKEKVFYSLMRESGYMHIQCTKPDTVGSALNDSPVGLAAYILEKFSTWTN
TEFRYLEDGGLERKFSLDDLLTNVMLYWTTATIVSSQRFYKENLGQGWMTQKHERMKV
YVPTGFSAFPSELMHTPEKWVRFKYPKLISYSYMARGGHFAAFEEPELLAQDIRKFLS
VLEQQ"
polyA_site 1625..1642
BASE COUNT 381 a 486 c 411 g 364 t
ORIGIN
1 gagaccgcgc gcctgcagcc gccggagcct gcgagccgag accgagccat gtggctagaa
61 atcctcctga cttcagtgct gggctttgcc atctactggt tcatctcccg ggacaaagag
121 gaaactttgc cacttgaaga tgggtggtgg ggcccaggca cgaggcccac aggcagggaa
181 gacgagagca tccacccttt caaggtggaa acgtcagatg aggagatcca cgacttacac
241 cagaggatcg ataagttccg tttcacccca cctttggagg acagctgctt ccactatggc
301 ttcaactcca actacctgaa gaaagtcatc tcctactggc ggaatgaatt caactggaag
361 aagcaggtgg agattctcaa tagataccct cacttcaaga ctaagattga agggctggac
421 atccacttca tccatgtgaa gcccccccag ctgcccgcag gccgtacccc gaagcccttg
481 ctgatggtgc atggctggcc tggctctttc tacgagtttt ataagatcat cccactcctg
541 actgacccca agaaccatgg cctgagcgat gagcacgttt ttgaagtcat ctgcccttcc
601 atccctggct atggcttttc agaggcatcc tccaagaagg ggttcaactc ggtggccacc
661 gccaggatct tctacaagct gatgctgcgg ctgggcttcc aggaattcta cattcaagga
721 ggggactggg gggccctgat ctgcaccaat atggcccagc tggtgcccag ccacgtgaaa
781 ggcctgcact tgaacatggc tttggtttta agcaacttct ctaccctgac ccttctcctg
841 ggacggcgtt tcgggaggtt tcttggctac actgagagag atgtggagct gctgtaccct
901 gtcaaggaga aggtcttcta cagcctgatg agggagagcg gctacatgca catccagtgc
961 accaagcccg acaccgtggg ctctgctctg aatgactctc ctgtgggtct ggctgcctat
1021 attctagaga agttttccac ctggaccaat acggaattcc gatacctgga ggatggaggc
1081 ctggaaagga agttctccct ggatgacctg ctgaccaacg tcatgcttta ctggacaaca
1141 gcaaccatcg tctcctccca gcgcttctac aaggagaacc tgggacaggg ctggatgacc
1201 cagaagcatg agcggatgaa ggtctacgtg cccactggct tctctgcctt cccttctgag
1261 ctaatgcaca cgcctgaaaa gtgggtgagg ttcaagtacc cgaagctcat ctcctattcc
1321 tacatggctc gtgggggcca ctttgcagcc tttgaggaac cagagctgct cgcccaggac
1381 atccgcaagt tcctgtcagt gctggagcag cagtgaccac cccacaccgc cccctgcctc
1441 cctccacaag tgccctccag gcttttcttt gggaagatac cccttttctg aggaatgagt
1501 ttgcctccgt cccctgccca tgctgggagc ccacacttac cccctcaccc ctccaagctc
1561 actccccaat ccccaactct gtgcaataag caatatggcc ttgatgataa atgactttac
1621 ttctaaaaaa aaaaaaaaaa aa
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