LOCUS AB173928 1993 bp mRNA linear PRI 06-MAR-2007
DEFINITION Macaca fascicularis brain cDNA clone: QmoA-11193, similar to human
butyrophilin, subfamily 2, member A1 (BTN2A1),transcript variant
2, mRNA, RefSeq: NM_078476.1.
ACCESSION AB173928
VERSION AB173928.1
KEYWORDS oligo capping; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 1993)
AUTHORS Chien,H.-C., Wang,H.-Y., Liu,Y.-L., Wu,K.-M., Ho,T.-C., Tsai,S.-F.
and Shen,C.-K.J.
TITLE Direct Submission
JOURNAL Submitted (19-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Che-Kun James Shen
Institute of Molecular Biology, Academia Sinica (IMB, AC); 128,
Section 2, Academy Road, Taipei 115, Taiwan
URL :http://www.imb.sinica.edu.tw
REFERENCE 2
AUTHORS Wang,H.-Y., Chien,H.-C., Osada,N., Hashimoto,K., Sugano,S.,
Gojobori,T., Chou,C.-K., Tsai,S.-F., Wu,C.-I. and Shen,C.-K.J.
TITLE Rate of Evolution in Brain-Expressed Genes in Humans and Other
Primates
JOURNAL PLoS Biol. 5, e13 (2007)
COMMENT The International consortium for macaque cDNA sequencing
and analysis consists of: Department of Virology and Human
Genome Center, Institute of Medical Science, The University of
Tokyo, Tokyo, Japan; Division of Genetic Resources, National
Institute of Infectious Diseases of Japan, Tokyo, Japan; National
Health Research Institute, Taipei, Taiwan; Institute of Molecular
Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
Evolution, University of Chicago, Chicago, IL, USA; Center for
Information Biology, National Institute of Genetics of Japan,
Mishima, Japan.
Clone distribution: clone distribution information can be
found at: http://www.nih.go.jp/yoken/genebank/
Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5' and 3' primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
oligo-capping method. Libraries were made from:
QccE: cerebellum cortex
QnpA: parietal lobe
QtrA: temporal lobe right
QflA: frontal lobe left
QmoA: medulla oblongata
QbsA: brain stem
QorA: occipital lobe right
QtsA: testis
Custom primers were used for 5' and 3'-end sequencing. The
full-insert sequencing was done by primer-walking method using ABI
DNA sequencer.
FEATURES Location/Qualifiers
source 1..1993
/clone="QmoA-11193"
/clone_lib="macaque cDNA library QmoA"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
/sex="male"
CDS 317..1150
/note="Homo sapiens butyrophilin, subfamily 2, member A1
(BTN2A1),transcript variant 2, mRNA, RefSeq: NM_078476.1"
/protein_id="BAE90990.1"
/translation="MEVRWFRSQFFPAVFVYKGGRERTEEQMEEYRGRTTFVSKDISR
GSVALVIHNVTAQENGTYRCYFQEGRSYDEAILHLVVAGLGSKPLIEMRGHEDGGIRL
ECISRGWYPKPLTVWRDPYGGIVPALKEVVTPDTDGLFMVTTAVIIRDKSMRNMSCSI
IVVFLMIIIAVCIYWINRLQKETKILSGEKEFVVETREIAVKELEKERVQKEKELQVK
EQLQEELRWRRTVLHAGDLWPATPHPSLAWLHWHAPAHLSYSVYPRSAVPELLCHAQE
E"
BASE COUNT 593 a 429 c 527 g 444 t
ORIGIN
1 gaggctgggg aagcccagtg gtccagatct cggttcctgc atctccagac atggcgacct
61 aggggaaggg gaagtacaat tttttctcct cttttgggac ggtttgcgtc tagtagtgcc
121 tgtgcccctg ggcagcttgg agagaagagg ggcgactgga aaatcgtcat ttcagaacca
181 gaggagaaaa gaaaacgcaa cgttaattct agaagcccag tttattgtgg tagggccaac
241 tgatcccatc ctggccacgg tcggagaaaa cactacgtta cgctgccatc tgtcacccga
301 gaaaaatgct gaggacatgg aggtgcggtg gttccggtct cagttcttcc ccgcagtgtt
361 tgtgtataag ggtggaagag agagaacaga ggagcagatg gaggagtacc gaggaagaac
421 cacctttgtg agcaaagaca tcagcagggg cagcgtggcc ctggtcatac acaacgtcac
481 agcccaagag aacggcacct accgctgtta cttccaagaa ggcaggtcct acgatgaggc
541 catcctgcac ctcgtggtgg caggactggg ctctaagccc ctcattgaaa tgaggggcca
601 cgaggacgga ggcatccggc tggagtgcat atctagaggg tggtacccaa agcccctcac
661 agtgtggagg gacccctacg gtgggattgt gcctgccctg aaggaggtcg tgacccctga
721 cacagacggc ctcttcatgg tcaccacagc tgtgatcatc agagataagt cgatgaggaa
781 catgtcctgc tctatcattg tggtctttct gatgataatc attgccgtat gcatctactg
841 gatcaacaga ctccaaaagg aaacaaagat tctgtcaggg gaaaaggagt ttgtagtgga
901 aacaagagaa attgctgtaa aggaactgga gaaggaacgt gtgcaaaaag agaaagaact
961 tcaagtaaaa gagcaacttc aagaagaatt gcgatggaga agaacagtct tacatgctgg
1021 ggacctctgg ccggcaacac cccacccaag ccttgcttgg ctacactggc atgccccagc
1081 tcacctgagt tatagcgtgt acccacgttc tgcagttcct gagctcttgt gccatgccca
1141 agaagaatga ggatacacta gacattgaag ggtgaggagg gatgagaaga attttatcaa
1201 gcgactgaac agcactcagt agagagggga tgctggcacc cctacccgaa ggcgggaaag
1261 ttccccctgt gtggctgggt ccaggcctgt tttacggact cataatggga agtgtgtgct
1321 gattggtttg tgagtatgca aaaaaggtta aagtgaagac accactcaaa ggtggccaca
1381 acgctgtaaa aaaccaatta ggaaagggtg agtatatgga aagtagttga agagtgggga
1441 ccaataagag gaaaatgcac caaacatgaa gacaagttct caacaagttc tcaatcaggg
1501 ctgaggattt accttgtagt ttgactttca ggctttaaac tgtcttcagc ttggaggtgg
1561 ggtttcacca gggacccatt gctacctgcc tttgcatctg actgcctcct gtctgctatc
1621 actaagtccc aaggtttgta gcaaggaccc cttacaaaga catcccccac agtctaccca
1681 aaggttaacc tcagaacgta tttcattgtc agagtgagaa acaaatacac gtgaaaatac
1741 agtatggcac aaaaaaggag caagaggtga agaaaggctc agtcacaccc aaaataattt
1801 tgtgaaaaat ataaaatatg cagttactga cagcactaac tctggatacc agaaaattct
1861 ataattcagt aatggatctg agacctgaat tttggaggta ctaaaataga ttcagtagat
1921 ctagttttca ttctgttcat actttcattt ggctctgtgg taaaagatgg taatataaaa
1981 aaaaaaaaaa aaa
//