LOCUS AB169531 1852 bp mRNA linear PRI 06-OCT-2006
DEFINITION Macaca fascicularis brain cDNA, clone: QccE-14518, similar to
human enolase 1, (alpha) (ENO1), mRNA, RefSeq: NM_001428.2.
ACCESSION AB169531
VERSION AB169531.1
KEYWORDS FLI_CDNA; oligo capping; fis (full insert sequence).
SOURCE Macaca fascicularis (crab-eating macaque)
ORGANISM Macaca fascicularis
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Cercopithecidae; Cercopithecinae; Macaca.
REFERENCE 1 (bases 1 to 1852)
AUTHORS Hashimoto,K., Kusuda,J. and Sugano,S.
TITLE Direct Submission
JOURNAL Submitted (18-MAR-2004) to the DDBJ/EMBL/GenBank databases.
Contact:Katsuyuki Hashimoto
National Institute of Infectious Diseases, Division of Genetic
Resources; 23-1, Toyama 1-chome, Shinjuku-ku, Tokyo 162-8640, Japan
URL :http://www.nih.go.jp/yoken/genebank/
REFERENCE 2
AUTHORS
CONSRTM International consortium for macaque cDNA sequencing and analysis
TITLE DNA sequences of macaque genes expressed in brain or testis and
its evolutionary implications
JOURNAL Unpublished (2004)
REFERENCE 3
AUTHORS Osada,N., Hirata,M., Tanuma,R., Kusuda,J., Hida,M., Suzuki,Y.,
Sugano,S., Gojobori,T., Shen,C.-K.J., Wu,C.I. and Hashimoto,K.
TITLE Substitution Rate and Structural Divergence of 5'UTR Evolution:
Comparative Analysis Between Human and Cynomolgus Monkey cDNAs
JOURNAL Mol. Biol. Evol. 22, 1976-1982 (2005)
COMMENT The International consortium for macaque cDNA sequencing
and analysis consists of:Department of Virology and Human
Genome Center, Institute of Medical Science, The University of
Tokyo, Tokyo, Japan; Division of Genetic Resources, National
Institute of Infectious Diseases of Japan, Tokyo, Japan; National
Health Research Institute, Taipei, Taiwan; Institute of Molecular
Biology, Academia Sinica, Taipei, Taiwan; Department of Ecology &
Evolution, University of Chicago, Chicago, IL, USA, Center for
Information Biology, National Institute of Genetics of Japan,
Mishima, Japan.
Clone distribution: clone distribution information can be
found at: http://www.nih.go.jp/yoken/genebank/
Lab host: TOP10
Vector: pME18S-FL3 (Acc.No. AB009864)
R. Site1: DraIII (CACTGTGTG)
R. Site2: DraIII (CACCATGTG)
Description: 1st strand cDNA was primed with an oligo(dT) primer
[ATGTGGCCTTTTTTTTTTTTTTTTT]; double-stranded cDNA was
synthesized using specific 5' and 3' primers and amplified
by PCR. The PCR product was digested with SfiI and size selection
was performed to exclude fragments <1.5kb.The SfiI-digested
PCR product was cloned into distinct DraIII sites of pME18S-FL3.
XhoI sites just outside the DraIII sites can be used to
isolate the cDNA insert. Libraries were constructed by
oligo-capping method. Libraries were made from:
QccE: cerebellum cortex
QnpA: parietal lobe
QtrA: temporal lobe right
QflA: frontal lobe left
QmoA: medulla oblongata
QbsA: brain stem
QorA: occipital lobe right
QtsA: testis
Custom primers were used for 5' and 3'-end sequencing. The
full-insert sequencing was done by primer-walking method using ABI
DNA sequencer.
FEATURES Location/Qualifiers
source 1..1852
/clone="QccE-14518"
/clone_lib="macaque cDNA library QccE"
/db_xref="taxon:9541"
/dev_stage="adult"
/mol_type="mRNA"
/organism="Macaca fascicularis"
/sex="male"
CDS 167..1471
/note="Homo sapiens enolase 1, (alpha) (ENO1), mRNA,
RefSeq: NM_001428.2"
/protein_id="BAE01613.1"
/translation="MSILKVHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIY
EALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTEN
KSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAG
NKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNIL
ENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQL
ADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVANPKRIAKAVNE
KSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQ
IKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK"
BASE COUNT 439 a 491 c 514 g 408 t
ORIGIN
1 agctgggcag gaagtccgag tgcggcgcgg acagtaggtg tggggagccg gagcgcatag
61 acctcgcaga cctcgcagac ctcgccggct tcacactcac ctcggtgtct gctgcacccg
121 ccgcttcttc tcctaggcca cgagacccag cggctagaaa ttcaccatgt ctattctcaa
181 ggtccatgcc agggagatct ttgactctcg tgggaatccc actgttgagg ttgatctctt
241 cacctcaaaa ggtctcttca gagctgctgt gcccagtggt gcttcaactg gtatctatga
301 ggccctagag ctccgggaca atgataagac tcgctatatg gggaagggtg tctcaaaggc
361 tgttgagcac atcaataaaa ctattgcgcc tgccctggtt agcaagaaac tgaacgtcac
421 agaacaagag aagattgaca aactgatgat agagatggat ggaacagaaa ataaatctaa
481 gtttggtgcg aacgccattc tgggggtgtc cctcgccgtc tgcaaagctg gtgctgttga
541 gaagggggtc cccctgtacc gccacatcgc tgacttggct ggcaactctg aagtcatcct
601 accagtcccg gctttcaatg tcatcaatgg cggttctcat gccggcaaca agctggccat
661 gcaggagttc atgatcctcc cagtcggtgc agcaaacttc agggaagcca tgcgcattgg
721 agcggaggtt taccacaacc tgaagaatgt catcaaggag aaatacggga aagatgccac
781 caatgtgggg gatgaaggcg ggtttgctcc caatatcctg gagaataaag aaggcctgga
841 gctgctgaag actgccattg ggaaagctgg ctacactgat aaggtggtca ttggcatgga
901 cgtagcagcc tccgagttct tcaggtctgg gaagtatgac ctggacttca agtctcccga
961 tgaccccagc aggtacatct cacctgatca gctggctgac ctgtacaagt ccttcatcaa
1021 ggactaccca gtggtgtcta tcgaagatcc ctttgaccag gatgactggg gagcttggca
1081 gaagttcacg gccagtgcag gaatccaggt agtcggggat gatctcacag tggccaaccc
1141 aaagaggatt gccaaggccg tgaacgagaa gtcctgcaac tgcctcctgc tcaaagtcaa
1201 ccagattggc tccgtgaccg agtcgcttca ggcgtgcaag ttggcccagg ccaatggttg
1261 gggcgtcatg gtgtctcatc gttctgggga gactgaagat accttcattg ctgacctggt
1321 tgtggggctg tgcactgggc agatcaagac tggtgcccct tgccgatctg agcgcttggc
1381 caagtacaac cagctcctca gaattgaaga ggagctgggc agcaaggcta agtttgccgg
1441 caggaacttc agaaaccccc tggccaagta agctgtgggc aggcaagccc ttcggtcacc
1501 tgttggctaa atagacccct ccccttgtgt cagctcgggc agcttgaggt cccgaccaac
1561 gcttgcaggg gtccctgcta gttagcgccc ctcacccgcc gccgtggagt ccgtgccgct
1621 tccttagaac ttctacagaa ccaagctccc cggagccctg tttggcagct ctagctttgc
1681 cgtcgtgtaa ttggcccaaa tcactgtttt tctcgcctta ctttccacca agtgtctgga
1741 gtcatgtgag cctcgtgtca tctccggggt ggccacaggc aagatgcctg gtggttttgt
1801 gcttaaaata aaaagcctca gtgacccatg agaaaaaaaa aaaaaaaaaa aa
//