LOCUS       AA933162                 502 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-E0-bp-d-08-0-UI.s2 UI-R-E0 Rattus norvegicus cDNA clone
            UI-R-E0-bp-d-08-0-UI 3' similar to gi|38099|emb|X00439|MMTCRP1
            M.musculus thymocyte mRNA fragment for T-cell receptor pseudogene
            (86T3), mRNA sequence.
ACCESSION   AA933162
VERSION     AA933162.1
DBLINK      BioSample: SAMN00155713
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     On Apr 24, 1998 this sequence version replaced gi:3087516.
            Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence tag present in the cDNA between the NotI site and the
            oligo-dT track served to identify it as a clone from the normalized
            adult 8-Day-Embryo library. cDNA Library Preparation: M. Fatima
            Bonaldo, Ph.D. Clone distribution: clones will be available through
            Research Genetics This clone is also available through the
            I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE
            ID=1769740
            Seq primer: M13 Forward
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-E0-bp-d-08-0-UI"
                     /clone_lib="SAMN00155713 UI-R-E0"
                     /dev_stage="embryonic"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: NotI; Site_2: EcoRI;
                     This library consists of a mixture of individually tagged
                     normalized libraries constructed from 8, 12 and 18-day
                     embryo. The tag is a string of 3-5 nucleotides present
                     between the Not I site and the oligo-dT track which allows
                     identification of the library of origin of a clone within
                     the mixture."
BASE COUNT          175 a           83 c           72 g          172 t
ORIGIN      
        1 tttttttttt tttttttcta taatgattgc cagttttatt cctctggcta agaaatccag
       61 aggtaattcc aggtttacat ttctttttaa tagttcatca ggagtttagt gcctttattg
      121 cctctacaag ctggttacaa gttaatttta attaactccc acttattaaa aaaatctgca
      181 gttctttgag acatgtattc attaggaaga tcaacataag caaaaggcaa aattaaaaca
      241 caccacacca aaaataagca gttaagtttt ttgatacgta taattataca tgtacacaca
      301 taattaggaa ttttcctata tatgtgagct gatcctaaat atgctgcttt tcaatctgct
      361 gtttaattgt atatttccat gcataagtgt gaaactttcc aactgaaaga taaacgtgga
      421 aaatgggtca aaaatccaaa atccaatgag atgctggtaa caacgaaact acctaaaaga
      481 aaataccgcc aggtaattat ta
//