LOCUS AA933162 502 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-E0-bp-d-08-0-UI.s2 UI-R-E0 Rattus norvegicus cDNA clone UI-R-E0-bp-d-08-0-UI 3' similar to gi|38099|emb|X00439|MMTCRP1 M.musculus thymocyte mRNA fragment for T-cell receptor pseudogene (86T3), mRNA sequence. ACCESSION AA933162 VERSION AA933162.1 DBLINK BioSample: SAMN00155713 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 502) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT On Apr 24, 1998 this sequence version replaced gi:3087516. Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to identify it as a clone from the normalized adult 8-Day-Embryo library. cDNA Library Preparation: M. Fatima Bonaldo, Ph.D. Clone distribution: clones will be available through Research Genetics This clone is also available through the I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE ID=1769740 Seq primer: M13 Forward POLYA=No. FEATURES Location/Qualifiers source 1..502 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-E0-bp-d-08-0-UI" /clone_lib="SAMN00155713 UI-R-E0" /dev_stage="embryonic" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: NotI; Site_2: EcoRI; This library consists of a mixture of individually tagged normalized libraries constructed from 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture." BASE COUNT 175 a 83 c 72 g 172 t ORIGIN 1 tttttttttt tttttttcta taatgattgc cagttttatt cctctggcta agaaatccag 61 aggtaattcc aggtttacat ttctttttaa tagttcatca ggagtttagt gcctttattg 121 cctctacaag ctggttacaa gttaatttta attaactccc acttattaaa aaaatctgca 181 gttctttgag acatgtattc attaggaaga tcaacataag caaaaggcaa aattaaaaca 241 caccacacca aaaataagca gttaagtttt ttgatacgta taattataca tgtacacaca 301 taattaggaa ttttcctata tatgtgagct gatcctaaat atgctgcttt tcaatctgct 361 gtttaattgt atatttccat gcataagtgt gaaactttcc aactgaaaga taaacgtgga 421 aaatgggtca aaaatccaaa atccaatgag atgctggtaa caacgaaact acctaaaaga 481 aaataccgcc aggtaattat ta //