LOCUS AA924190 581 bp mRNA linear EST 27-JAN-2011
DEFINITION UI-R-E0-bp-d-07-0-UI.s3 UI-R-E0 Rattus norvegicus cDNA clone
UI-R-E0-bp-d-07-0-UI 3' similar to gi|38099|emb|X00439|MMTCRP1
M.musculus thymocyte mRNA fragment for T-cell receptor pseudogene
(86T3) gi|201316|gb|K01081|MUSTCBXC Mouse T-cell receptor
beta-chain mRNA (pseudogene), clone 86T3. gi|588929|gb|I08357|
Sequence 4 from Paten, mRNA sequence.
ACCESSION AA924190
VERSION AA924190.1
DBLINK BioSample: SAMN00155713
KEYWORDS EST.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 581)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
PUBMED 8889548
COMMENT On Apr 21, 1998 this sequence version replaced gi:3071326.
Contact: Soares, MB
Cancer Biology & Epigenomics Program
Children's Memorial Research Center
2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
Tel: 773 755 6551
Fax: 773 755 6378
Email: mbsoares@childrensmemorial.org
The sequence tag present in the cDNA between the NotI site and the
oligo-dT track served to identify it as a clone from the normalized
adult 8-Day-Embryo library. cDNA Library Preparation: M. Fatima
Bonaldo, Ph.D. Clone distribution: clones will be available through
Research Genetics
Seq primer: M13 Forward.
FEATURES Location/Qualifiers
source 1..581
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="Sprague-Dawley"
/db_xref="taxon:10116"
/clone="UI-R-E0-bp-d-07-0-UI"
/clone_lib="SAMN00155713 UI-R-E0"
/dev_stage="embryonic"
/lab_host="DH10B (Life Technologies)"
/note="Vector: pT7T3D-PacI; Site_1: NotI; Site_2: EcoRI;
This library consists of a mixture of individually tagged
normalized libraries constructed from 8, 12 and 18-day
embryo. The tag is a string of 3-5 nucleotides present
between the Not I site and the oligo-dT track which allows
identification of the library of origin of a clone within
the mixture."
BASE COUNT 220 a 93 c 82 g 186 t
ORIGIN
1 tttttttttt tttttttcta taatgattgc cagttttatt cctctggcta agaaatccag
61 aggtaattcc aggtttacat ttctttttaa tagttcatca ggagtttagt gcctttattg
121 cctctacaag ctggttacaa attaatttta attaactccc acttattaaa aaaatctgca
181 gttctttgag acatgtattc attaaaaaaa tcaacataag caaaaggcaa aattaaaaca
241 caccacacca aaaataagca gttaagtttt ttgatacgta taattataca tgtacacaca
301 taattaggaa ttttcctata tatgtgagct gatcctaaat atgctgcttt tcaatctgct
361 gtttaattgt atatttccat gcataagtgt gaaactttcc aactgaaaga taaacgtgga
421 aaatgggtca aaaatccaaa atccaatgag atgctggtaa caacgaaact acctaaaaga
481 aaataccgcc aggtaattat tattaaaagt aaaatgaagt tcaaagatac atcagaaaaa
541 tgctaagaga aagaaagcag cagccacatt tcaatagaga a
//