LOCUS       AA900000                 480 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-E0-cr-f-05-0-UI.s2 UI-R-E0 Rattus norvegicus cDNA clone
            UI-R-E0-cr-f-05-0-UI 3' similar to gi|57687|emb|X58200|RRRPL23 Rat
            mRNA for ribosomal protein L23, mRNA sequence.
VERSION     AA900000.1
DBLINK      BioSample: SAMN00155713
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     On Apr 7, 1998 this sequence version replaced gi:3035354.
            Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            The sequence tag present in the cDNA between the NotI site and the
            oligo-dT track served to identify it as a clone from the normalized
            adult 12-Day-Embryo library. cDNA Library Preparation: M. Fatima
            Bonaldo, Ph.D. Clone distribution: clones will be available through
            Research Genetics This clone is also available through the
            I.M.A.G.E. Consortium at LLNL ( IMAGE
            Seq primer: M13 Forward
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Rattus norvegicus"
                     /clone_lib="SAMN00155713 UI-R-E0"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: NotI; Site_2: EcoRI;
                     This library consists of a mixture of individually tagged
                     normalized libraries constructed from 8, 12 and 18-day
                     embryo. The tag is a string of 3-5 nucleotides present
                     between the Not I site and the oligo-dT track which allows
                     identification of the library of origin of a clone within
                     the mixture."
BASE COUNT          111 a          129 c           89 g          151 t
        1 tttttttttt tttttttcag agagaagact ttaatgaata tattttacaa acacactgga
       61 gaatcaggca atgcttcctg cattggatgc aatcctgggc cacaagtctg cacactcctt
      121 tgcaactgga cctgtgatag cagaaccttt catctcgcct ttattgttta ctatgacccc
      181 tgcattatct tcaaaataaa ggaacacccc gtcttttctt cgatatgact ttcgttgtcg
      241 aatcaccact gcaggatgta cctttttcct tagttctggt ttgcctttct taactgtggc
      301 catcaccatg tcgcccacac cagcagcagg aagtctgttc agccgtccct tgattccctt
      361 cacagagatg atatacaggt ttttggctcc tgtgttgtca gcacagttga tcacagctcc
      421 taccggaaga cccacggaaa tccggaattt cgctccggag gacccaccac gtcctcgctt