LOCUS AA900000 480 bp mRNA linear EST 27-JAN-2011
DEFINITION UI-R-E0-cr-f-05-0-UI.s2 UI-R-E0 Rattus norvegicus cDNA clone
UI-R-E0-cr-f-05-0-UI 3' similar to gi|57687|emb|X58200|RRRPL23 Rat
mRNA for ribosomal protein L23, mRNA sequence.
ACCESSION AA900000
VERSION AA900000.1
DBLINK BioSample: SAMN00155713
KEYWORDS EST.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 480)
AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B.
TITLE Normalization and subtraction: two approaches to facilitate gene
discovery
JOURNAL Genome Res. 6 (9), 791-806 (1996)
PUBMED 8889548
COMMENT On Apr 7, 1998 this sequence version replaced gi:3035354.
Contact: Soares, MB
Cancer Biology & Epigenomics Program
Children's Memorial Research Center
2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
Tel: 773 755 6551
Fax: 773 755 6378
Email: mbsoares@childrensmemorial.org
The sequence tag present in the cDNA between the NotI site and the
oligo-dT track served to identify it as a clone from the normalized
adult 12-Day-Embryo library. cDNA Library Preparation: M. Fatima
Bonaldo, Ph.D. Clone distribution: clones will be available through
Research Genetics This clone is also available through the
I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE
ID=1770363
Seq primer: M13 Forward
POLYA=No.
FEATURES Location/Qualifiers
source 1..480
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="Sprague-Dawley"
/db_xref="taxon:10116"
/clone="UI-R-E0-cr-f-05-0-UI"
/clone_lib="SAMN00155713 UI-R-E0"
/dev_stage="embryonic"
/lab_host="DH10B (Life Technologies)"
/note="Vector: pT7T3D-PacI; Site_1: NotI; Site_2: EcoRI;
This library consists of a mixture of individually tagged
normalized libraries constructed from 8, 12 and 18-day
embryo. The tag is a string of 3-5 nucleotides present
between the Not I site and the oligo-dT track which allows
identification of the library of origin of a clone within
the mixture."
BASE COUNT 111 a 129 c 89 g 151 t
ORIGIN
1 tttttttttt tttttttcag agagaagact ttaatgaata tattttacaa acacactgga
61 gaatcaggca atgcttcctg cattggatgc aatcctgggc cacaagtctg cacactcctt
121 tgcaactgga cctgtgatag cagaaccttt catctcgcct ttattgttta ctatgacccc
181 tgcattatct tcaaaataaa ggaacacccc gtcttttctt cgatatgact ttcgttgtcg
241 aatcaccact gcaggatgta cctttttcct tagttctggt ttgcctttct taactgtggc
301 catcaccatg tcgcccacac cagcagcagg aagtctgttc agccgtccct tgattccctt
361 cacagagatg atatacaggt ttttggctcc tgtgttgtca gcacagttga tcacagctcc
421 taccggaaga cccacggaaa tccggaattt cgctccggag gacccaccac gtcctcgctt
//