LOCUS AA675381 244 bp mRNA linear EST 28-NOV-1997 DEFINITION vm72h01.s1 Knowles Solter mouse 2 cell Mus musculus cDNA clone IMAGE:1003825 5', mRNA sequence. ACCESSION AA675381 VERSION AA675381.1 DBLINK BioSample: SAMN00155461 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 244) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:568041. FEATURES Location/Qualifiers source 1..244 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J x DBA/2J F1" /db_xref="taxon:10090" /clone="IMAGE:1003825" /tissue_type="embryo" /clone_lib="SAMN00155461 Knowles Solter mouse 2 cell" /dev_stage="2-cell" /lab_host="DH10B" /note="Organ: embryo; Vector: pBluescribe (modified); Site_1: MluI; Site_2: SalI; Cloned unidirectionally from mRNA prepared from 13,500 2-cell stage embryos. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the MluI/SalI sites of a modified pBluescribe vector using commercial linkers (NEB). Average insert size: 1.2 kb." BASE COUNT 62 a 64 c 80 g 38 t ORIGIN 1 gaaaggaagg cagggcagcg aaacaagaaa gcctgagaag gggggatggg ggatggtgaa 61 caactgctct gggagggaca aaccctgcac tggcctgacc ttcaggggaa gctgcctacc 121 tgcctggtgt ttccactggc caacaatcca gagcttagga gagcaccctg gactggggga 181 ggtcagaccg tgttggctgg ggccacccac cagtcaccat ttgctggact ataacccaca 241 gacc //