LOCUS       AA675381                 244 bp    mRNA    linear   EST 28-NOV-1997
DEFINITION  vm72h01.s1 Knowles Solter mouse 2 cell Mus musculus cDNA clone
            IMAGE:1003825 5', mRNA sequence.
VERSION     AA675381.1
DBLINK      BioSample: SAMN00155461
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium ( for further information.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Mus musculus"
                     /strain="C57BL/6J x DBA/2J F1"
                     /clone_lib="SAMN00155461 Knowles Solter mouse 2 cell"
                     /note="Organ: embryo; Vector: pBluescribe (modified);
                     Site_1: MluI; Site_2: SalI; Cloned unidirectionally from
                     mRNA prepared from 13,500 2-cell stage embryos. Primer:
                     SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs
                     were cloned into the MluI/SalI sites of a modified
                     pBluescribe vector using commercial linkers (NEB). Average
                     insert size: 1.2 kb."
BASE COUNT           62 a           64 c           80 g           38 t
        1 gaaaggaagg cagggcagcg aaacaagaaa gcctgagaag gggggatggg ggatggtgaa
       61 caactgctct gggagggaca aaccctgcac tggcctgacc ttcaggggaa gctgcctacc
      121 tgcctggtgt ttccactggc caacaatcca gagcttagga gagcaccctg gactggggga
      181 ggtcagaccg tgttggctgg ggccacccac cagtcaccat ttgctggact ataacccaca
      241 gacc