LOCUS AA537612 480 bp mRNA linear EST 29-JUL-1997
DEFINITION vj98g07.r1 Knowles Solter mouse blastocyst B1 Mus musculus cDNA
clone IMAGE:945180 5', mRNA sequence.
ACCESSION AA537612
VERSION AA537612.1
DBLINK BioSample: SAMN00155449
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 480)
AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
Waterston,R.
TITLE The WashU-HHMI Mouse EST Project
JOURNAL Unpublished
COMMENT Contact: Marra M/Mouse EST Project
WashU-HHMI Mouse EST Project
Washington University School of MedicineP
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: mouseest@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
MGI:542036
High quality sequence stop: 373.
FEATURES Location/Qualifiers
source 1..480
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J x DBA/2J F1"
/db_xref="taxon:10090"
/clone="IMAGE:945180"
/tissue_type="blastocyst"
/clone_lib="SAMN00155449 Knowles Solter mouse blastocyst
B1"
/dev_stage="embryo (pre-implantation)"
/lab_host="DH10B"
/note="Organ: embryo; Vector: pSPORT; Site_1: NotI;
Site_2: SalI; Cloned unidirectionally from mRNA prepared
from 800 blastocysts. Primer: SalI(dT):
5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned
into the NotI/SalI sites of a pSPORT vector (Life
Technologies). Two different size selections: B1 (larger
inserts) and B3."
BASE COUNT 101 a 107 c 93 g 179 t
ORIGIN
1 tcttatgtcc tgtttcagcc gtgagatcag tccttatagg agctagaaca aagctgcgaa
61 tctctcagtt gctaaacctc acccttgtga acggatccag caagtgttct tgtctcttga
121 agatacacta acatggatgg gttcctttgg aagacatccc tggtgctcta gtgacagcac
181 aggccgtgtg tttcctccta ctccgtattt tctatctctg ctgtgaggac ttacagccag
241 tgggtggcgc tgtaataccg gagactcatt tgttctgttg ccactaagtg tttttattat
301 gggactattt ccttacatca tatgctctga attctctctc tctctctctc tctctgtaat
361 aggtgctaga aacttaatga ctggtgttaa tactttctga cttaactttt atgtgttttc
421 tttgattatt tataatgact gtttgatatt ttcttttgta tccttctgac cctgctgcaa
//