LOCUS AA516946 530 bp mRNA linear EST 14-JUL-1997
DEFINITION vh85c03.r1 Knowles Solter mouse E6 5d whole embryo Mus musculus
cDNA clone IMAGE:893764 3', mRNA sequence.
ACCESSION AA516946
VERSION AA516946.1
DBLINK BioSample: SAMN00155506
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 530)
AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
Waterston,R.
TITLE The WashU-HHMI Mouse EST Project
JOURNAL Unpublished
COMMENT Contact: Marra M/Mouse EST Project
WashU-HHMI Mouse EST Project
Washington University School of MedicineP
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: mouseest@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
MGI:521724
High quality sequence stop: 481.
FEATURES Location/Qualifiers
source 1..530
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J x DBA/2J F1"
/db_xref="taxon:10090"
/clone="IMAGE:893764"
/clone_lib="SAMN00155506 Knowles Solter mouse E6 5d whole
embryo"
/dev_stage="embryo (post-implantation)"
/lab_host="DH10B"
/note="Vector: pBluescribe (modified); Site_1: MluI;
Site_2: SalI; Cloned unidirectionally from mRNA prepared
from 13,500 2-cell stage embryos. Primer: SalI(dT):
5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned
into the MluI/SalI sites of a modified pBluescribe vector
using commercial linkers (NEB)."
BASE COUNT 154 a 82 c 69 g 225 t
ORIGIN
1 aaactattag ataccagcat cccactggta ttcttagtat aagaattgtg atgtttggtc
61 ttgttctata tttttaatgt atttcactgg ttttaaaatt tctgacaact taaaatattg
121 cttttatcaa acactatatt tttctttctt tgaatttttg tacctttgtg atattgtaga
181 tattctaata tgttctactt accgaaacta atagttgcct aaaattttct atccatagtt
241 tgttttgctt tgtattaatt acaaaactaa tgggattctt tctcctttgg gtttcctctc
301 cccttcgtca gagagagggt tttactgttc agtgttgaag cacaacatca tcttcccatc
361 actggaatag tcaactcagt gtcatttttt aatgaaacaa aatgagttca tacctcattg
421 ctttgattct aattaaataa ctatagaaaa atgtgttagt tttttatgta atatatttcc
481 atagactttg tcttagtatt tttactatga taagcttaca aataatatat
//