LOCUS       AA437664                 552 bp    mRNA    linear   EST 30-MAY-1997
DEFINITION  ve31b11.r1 Ko mouse embryo 11 5dpc Mus musculus cDNA clone
            IMAGE:819741 5', mRNA sequence.
ACCESSION   AA437664
VERSION     AA437664.1
DBLINK      BioSample: SAMN00155444
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:488021
            High quality sequence stop: 477.
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:819741"
                     /sex="pooled"
                     /tissue_type="embryo"
                     /clone_lib="SAMN00155444 Ko mouse embryo 11 5dpc"
                     /dev_stage="11.5dpc"
                     /lab_host="DH10B"
                     /note="Organ: embryo; Vector: pSPORT1; Site_1: SalI;
                     Site_2: NotI; Total RNAs were extracted from 11.5 dpc
                     embryos (excluding placenta and yolk sac). The
                     double-stranded cDNA was synthesized with an oligo (dT)-1
                     primer GAGAGAGACTAGTTCTAGATCGCGAGCGGCCGCTTTTTTTTTTTTTTTTTT
                     3'. The cDNAs were ligated to LL-Sal3A: 5'
                     GCTATTGACGTCGACTATCC 3' and LL-Sal3B: 5' GGATAGTCGACGTCAAT
                     3'. The cDNAs were size-selected and amplified by
                     long-range PCR using Ex Taq polymerase for 18 cycles. The
                     PCR-amplifiable cDNA mixture went through one round of
                     equalization and was digested with SalI/NotI and cloned
                     into the SalI/NotI sites of the pSPORT1 plasmid vector
                     (Life Technologies). The library was constructed by Dr.
                     Minoru S. H. Ko and Dr. Xiaohong Wang."
BASE COUNT          149 a          118 c          120 g          165 t
ORIGIN      
        1 ctacaggctg gtggggaaag caggctttga acaggagtta caagttgtag catgtgcttt
       61 gtagatgaaa gccacaaaat gccacctgga ttttgattga caagactgac tgaatccttc
      121 tgtctgtccc tgccctgtgt tagtctggtc tgtaccctct tagcctcttt aatcttcctt
      181 ctgtctcctg gaccacctgc tgattaccag atagtatggc ctagagttaa tagaatggac
      241 agatctggtt ttcagggttg gccttttcgc ttagtttggt ctagtccaat ttttaatctg
      301 agaagcgggt aactggggaa acggctcagt gattgggagc actagctact cttgcaaaga
      361 acccagattc aattcctaac atctatatgg ttactcaaca gttccagagt atccctttcc
      421 cggccttgga gggaccaggc aaacgcatgg tacacagaca tgcagataaa acacccatgc
      481 atataaaaca tttttaaata attttaagtc tgagttttgg ttgctaattt atgaaatgaa
      541 aacaattcca ac
//