LOCUS AA422765 454 bp mRNA linear EST 16-OCT-1997
DEFINITION vd48e05.s1 Knowles Solter mouse 2 cell Mus musculus cDNA clone
IMAGE:803840 5', mRNA sequence.
ACCESSION AA422765
VERSION AA422765.1
DBLINK BioSample: SAMN00155461
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 454)
AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
Waterston,R.
TITLE The WashU-HHMI Mouse EST Project
JOURNAL Unpublished
COMMENT Contact: Marra M/Mouse EST Project
WashU-HHMI Mouse EST Project
Washington University School of MedicineP
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: mouseest@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
MGI:480184
Seq primer: -40m13 fwd. ET from Amersham
High quality sequence stop: 419.
FEATURES Location/Qualifiers
source 1..454
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J x DBA/2J F1"
/db_xref="taxon:10090"
/clone="IMAGE:803840"
/tissue_type="embryo"
/clone_lib="SAMN00155461 Knowles Solter mouse 2 cell"
/dev_stage="2-cell"
/lab_host="DH10B"
/note="Organ: embryo; Vector: pBluescribe (modified);
Site_1: MluI; Site_2: SalI; Cloned unidirectionally from
mRNA prepared from 13,500 2-cell stage embryos. Primer:
SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs
were cloned into the MluI/SalI sites of a modified
pBluescribe vector using commercial linkers (NEB). Average
insert size: 1.2 kb."
BASE COUNT 116 a 92 c 108 g 138 t
ORIGIN
1 caaaaataaa aaaagtgtgt gagccaccac actctgcgat actagaagac atttgatgtc
61 atgcttaagg gtggagttgt attaatgata ttaattagtc tgtcgctaga tgttgactga
121 ggttgaaatt gtgtctccag cttgttcctc tggtaccact tctgcagatt tacacctgca
181 taatcgattt atctagtaag ctgttctgaa tagcagggct caggtcttcc atactcgctt
241 ttgagggggt aacagaagtt gtaagagttg tgccagagcc atcaccagct tgccttttgt
301 ttggctgata gagacatgaa gtcattgatc taagttggaa gatggcaggg tttgttttag
361 atagcccaag ctagccttga gccactaatc ctcctgcctc tgctccccaa gtactgggat
421 tgcaggctga cctaaaggtg atttccttac ggac
//