LOCUS AA400000 502 bp mRNA linear EST 09-NOV-1997 DEFINITION zu68g08.r1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:743198 5', mRNA sequence. ACCESSION AA400000 VERSION AA400000.1 DBLINK BioSample: SAMN00155229 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 502) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-NCI human EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 577 Std Error: 0.00 Seq primer: -28m13 rev2 ET from Amersham High quality sequence stop: 419. FEATURES Location/Qualifiers source 1..502 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:5930321" /db_xref="taxon:9606" /clone="IMAGE:743198" /sex="male" /clone_lib="SAMN00155229 Soares_testis_NHT" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from mRNA obtained from Clontech Laboratories, Inc., and primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization to Cot5, and was constructed by Bento Soares and M. Fatima Bonaldo." BASE COUNT 154 a 101 c 112 g 135 t ORIGIN 1 gtaagacttt aacccatgag atcggacaca tatttggact gcgacactgc cagtggcttg 61 catgcctcat gcaaggctcc aaccacttgg aagaagctga ccggcgccct ctaaaccttt 121 gccctatctg tttgcacaag ttgcagtgtg ctgttggctt cacattgtag aaagatacaa 181 agcactggtg aggtggattg atgatgaatc ttctgacaca cctggagcaa ctccagaaca 241 cagtcacgag gataatggga atttaccgaa acccgtggaa gcctttaagg aatggaaaga 301 gtggataata aaatgcctgg ctgttctcca aaaatgagga ccttcaaata ggagtgattg 361 aaataaataa ctacttgcat gttatgcttt catttgggtg gaatacttca ttggaataaa 421 ctactgatct tgtgctgtgt caaagtaaca gactagaacc ttctttcaag tacctgaatt 481 gaaatgaaac tcattttgaa ta //