LOCUS AA200000 387 bp mRNA linear EST 17-FEB-1997 DEFINITION mu06f08.r1 Soares mouse 3NbMS Mus musculus cDNA clone IMAGE:638631 5' similar to TR:G852070 G852070 RB109.BRAIN SPECIFIC PROTEIN, mRNA sequence. ACCESSION AA200000 VERSION AA200000.1 DBLINK BioSample: SAMN00155158 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 387) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:390623 Seq primer: -28M13 rev2 from Amersham High quality sequence stop: 385. FEATURES Location/Qualifiers source 1..387 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="taxon:10090" /clone="IMAGE:638631" /sex="male" /tissue_type="Spleen" /clone_lib="SAMN00155158 Soares mouse 3NbMS" /dev_stage="4 weeks" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTGTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. RNA provided by Dr. Bertrand Jordan. Library went through three rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 93 a 101 c 134 g 59 t ORIGIN 1 cgctaaagca ggagctgcag gtgctatctg agaggtactc gcagaagtgc ctggagatcg 61 gggccctgac aaggcaggca gaagagcgtg cacaccctgc ggcgctgcca caagagggcc 121 aggagctgct gcgccacaac caggagctgc actcccacct gtccgaggag atcgaccgtc 181 tgcgcacttc atcgcctcgc agggcacagg caacagctgc gggcgccgaa tgagaggagt 241 tcctgtgagc tggaggtgct gctgcgagtg aaggagaatg aactccagta cctgaagaag 301 gaggtgcagt gtctccgaga tgagctccag gtgatacaga aggacaagcg cttcacgggg 361 aagtaccagg atgtgtacgt ggagctg //