LOCUS       AA200000                 387 bp    mRNA    linear   EST 17-FEB-1997
DEFINITION  mu06f08.r1 Soares mouse 3NbMS Mus musculus cDNA clone IMAGE:638631
            5' similar to TR:G852070 G852070 RB109.BRAIN SPECIFIC PROTEIN, mRNA
            sequence.
ACCESSION   AA200000
VERSION     AA200000.1
DBLINK      BioSample: SAMN00155158
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:390623
            Seq primer: -28M13 rev2 from Amersham
            High quality sequence stop: 385.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:638631"
                     /sex="male"
                     /tissue_type="Spleen"
                     /clone_lib="SAMN00155158 Soares mouse 3NbMS"
                     /dev_stage="4 weeks"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTGTTTTTTTTTTTTTTTTTTTTTTTT
                     3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. RNA
                     provided by Dr. Bertrand Jordan. Library went through
                     three rounds of normalization, and was constructed by
                     Bento Soares and M.Fatima Bonaldo."
BASE COUNT           93 a          101 c          134 g           59 t
ORIGIN      
        1 cgctaaagca ggagctgcag gtgctatctg agaggtactc gcagaagtgc ctggagatcg
       61 gggccctgac aaggcaggca gaagagcgtg cacaccctgc ggcgctgcca caagagggcc
      121 aggagctgct gcgccacaac caggagctgc actcccacct gtccgaggag atcgaccgtc
      181 tgcgcacttc atcgcctcgc agggcacagg caacagctgc gggcgccgaa tgagaggagt
      241 tcctgtgagc tggaggtgct gctgcgagtg aaggagaatg aactccagta cctgaagaag
      301 gaggtgcagt gtctccgaga tgagctccag gtgatacaga aggacaagcg cttcacgggg
      361 aagtaccagg atgtgtacgt ggagctg
//