LOCUS A23334 5620 bp DNA linear PAT 01-APR-1995
DEFINITION PVE108 plasmid DNA.
ACCESSION A23334
VERSION A23334.1
KEYWORDS .
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
REFERENCE 1 (bases 1 to 5620)
AUTHORS
TITLE STAMEN-SPECIFIC PROMOTERS FROM RICE
JOURNAL Patent number WO9213956-A1/9, 20-AUG-1992.
FEATURES Location/Qualifiers
source 1..5620
/organism="Escherichia coli"
/plasmid="pVE108"
/mol_type="unassigned DNA"
/db_xref="taxon:562"
misc_feature 1..395
/note="pUC18 derived sequence"
misc_feature 396..802
/note="Agrobacterium T-DNA derived sequence"
/note="contains polyA site from nopaline gene"
regulatory 1139..1683
/note="tapetum-specific"
/note="Nicotiana tabacum derived sequence"
/regulatory_class="promoter"
regulatory 1684..2516
/note="35S3"
/note="Califlower mosaic virus isolate CabbB-JI derived
sequence"
/regulatory_class="promoter"
CDS 2517..3068
/transl_table=11
/gene="bar"
/product="phosphinotricin transferase"
/protein_id="CAA01674.1"
/translation="MDPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQ
EWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGL
GSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHD
VGFWQLDFSLPVPPRPVLPVTEI"
misc_feature 3069..3356
/note="Agrobacterium T-DNA derived sequence"
/note="contains polyA site from nopaline synthase gene"
misc_feature 3357..5620
/note="pUC18 derived sequence"
BASE COUNT 1498 a 1359 c 1329 g 1430 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
421 cttcccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct
481 atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc
541 atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca
601 gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt
661 gccaaatgtt tgaacgatct gcttcggatc ctctagagnn nnccggaaag tgaaattgac
721 cgatcagagt ttgaagaaaa atttattaca cactttatgt aaagctgaaa aaaacggcct
781 ccgcaggaag ccgttttttt cgttatctga tttttgtaaa ggtctgataa tggtccgttg
841 ttttgtaaat cagccagtcg cttgagtaaa gaatccggtc tgaatttctg aagcctgatg
901 tatagttaat atccgcttca cgccatgttc gtccgctttt gcccgggagt ttgccttccc
961 tgtttgagaa gatgtctccg ccgatgcttt tccccggagc gacgtctgca aggttccctt
1021 ttgatgccac ccagccgagg gcttgtgctt ctgattttgt aatgtaatta tcaggtagct
1081 tatgatatgt ctgaagataa tccgcaaccc cgtcaaacgt gttgataacc ggtaccatgg
1141 tagctaattt ctttaagtaa aaactttgat ttgagtgatg atgttgtact gttacacttg
1201 caccacaagg gcatatatag agcacaagac atacacaaca acttgcaaaa ctaacttttg
1261 ttggagcatt tcgaggaaaa tggggagtag caggctaatc tgagggtaac attaaggttt
1321 catgtattaa tttgttgcaa acatggactt agtgtgagga aaaagtacca aaattttgtc
1381 tcaccctgat ttcagttatg gaaattacat tatgaagctg tgctagagaa gatgtttatt
1441 ctagtccagc cacccacctt atgcaagtct gcttttagct tgattcaaaa actgatttaa
1501 tttacattgc taaatgtgca tacttcgagc ctatgtcgct ttaattcgag taggatgtat
1561 atattagtac ataaaaaatc atgtttgaat catctttcat aaagtgacaa gtcaattgtc
1621 ccttcttgtt tggcactata ttcaatctgt taatgcaaat tatccagtta tacttagcta
1681 gatcctacgc agcaggtctc atcaagacga tctacccgag taacaatctc caggagatca
1741 aataccttcc caagaaggtt aaagatgcag tcaaaagatt caggactaat tgcatcaaga
1801 acacagagaa agacatattt ctcaagatca gaagtactat tccagtatgg acgattcaag
1861 gcttgcttca taaaccaagg caagtaatag agattggagt ctctaaaaag gtagttccta
1921 ctgaatctaa ggccatgcat ggagtctaag attcaaatcg aggatctaac agaactcgcc
1981 gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa gaagaaaatc
2041 ttcgtcaaca tggtggagca cgacactctg gtctactcca aaaatgtcaa agatacagtc
2101 tcagaagacc aaagggctat tgagactttt caacaaagga taatttcggg aaacctcctc
2161 ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc
2221 tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatgc ctctgccgac
2281 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
2341 accacgtctt caaagcaagt ggattgatgt gacatctcca ctgacgtaag ggatgacgca
2401 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
2461 aggacacgct gaaatcacca gtctctctct ataaatctat ctctctctct ataaccatgg
2521 acccagaacg acgcccggcc gacatccgcc gtgccaccga ggcggacatg ccggcggtct
2581 gcaccatcgt caaccactac atcgagacaa gcacggtcaa cttccgtacc gagccgcagg
2641 aaccgcagga gtggacggac gacctcgtcc gtctgcggga gcgctatccc tggctcgtcg
2701 ccgaggtgga cggcgaggtc gccggcatcg cctacgcggg cccctggaag gcacgcaacg
2761 cctacgactg gacggccgag tcgaccgtgt acgtctcccc ccgccaccag cggacgggac
2821 tgggctccac gctctacacc cacctgctga agtccctgga ggcacagggc ttcaagagcg
2881 tggtcgctgt catcgggctg cccaacgacc cgagcgtgcg catgcacgag gcgctcggat
2941 atgccccccg cggcatgctg cgggcggccg gcttcaagca cgggaactgg catgacgtgg
3001 gtttctggca gctggacttc agcctgccgg taccgccccg tccggtcctg cccgtcaccg
3061 agatctgatc tcacgcgtct aggatccgaa gcagatcgtt caaacatttg gcaataaagt
3121 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
3181 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
3241 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
3301 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga agatcctcta
3361 gagtcgacct gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga
3421 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
3481 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
3541 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
3601 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
3661 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
3721 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
3781 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
3841 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
3901 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
3961 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
4021 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
4081 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
4141 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
4201 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
4261 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
4321 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
4381 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
4441 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
4501 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
4561 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
4621 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
4681 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
4741 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
4801 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
4861 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
4921 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
4981 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
5041 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
5101 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
5161 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
5221 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
5281 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
5341 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
5401 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
5461 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
5521 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
5581 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
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