LOCUS Y00547 1507 bp DNA linear ENV 13-FEB-1995
DEFINITION Plasmid R27 replication region.
ACCESSION Y00547
VERSION Y00547.1
KEYWORDS origin of replication.
SOURCE uncultured bacterium
ORGANISM uncultured bacterium
Bacteria; environmental samples.
REFERENCE 1 (bases 1 to 1507)
AUTHORS Saul D., Lane D., Bergquist P.L.
TITLE A replication region of the IncHI plasmid, R27, is highly
homologous with the RepFIA replicon of F
JOURNAL Mol. Microbiol. 2(2), 219-225(1988).
PUBMED 3288833
COMMENT Draft entry and printed sequence for [1] kindly provided by D.Saul,
15-DEC-1988.
FEATURES Location/Qualifiers
source 1..1507
/organism="uncultured bacterium"
/plasmid="R27"
/environmental_sample
/mol_type="genomic DNA"
/db_xref="taxon:77133"
repeat_region 352..371
/note="inc repeat"
repeat_region 373..391
/note="inc repeat"
repeat_region 406..424
/note="inc repeat"
repeat_region 428..446
/note="inc repeat"
regulatory 482..486
/regulatory_class="minus_35_signal"
regulatory 504..509
/regulatory_class="minus_10_signal"
CDS 543..1298
/transl_table=11
/product="protein E"
/db_xref="GOA:P62538"
/db_xref="InterPro:IPR000525"
/db_xref="InterPro:IPR036388"
/db_xref="InterPro:IPR036390"
/db_xref="UniProtKB/Swiss-Prot:P62538"
/protein_id="CAA68616.1"
/translation="MAEIAVINHKKRKNSPRIVQSNELTEAAYSLSRDQKRLLYLFVH
QIRKSDGSLQEHDGICEIHVAKYAETFGLTSAEASKDIRQALKGFAGKEVVFYRPEED
AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
YAMRLYESLCQYRKPDGSGVVSLKIDWIMERYQLPQSYQRMPDFRRRFLKASVDEINS
RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTIE"
repeat_region 1300..1483
/note="repeats of about 50 bp"
BASE COUNT 387 a 351 c 360 g 409 t
ORIGIN
1 gagctcacgg atttcaattt gttccggggt aatgggggag gcttttggtg tttttccctg
61 ccgctcatca cgtaattaat aggctgtatg tctaacttct ggccacagat ataatcgagc
121 cagcagtgct cgccgcagcc gagcgacagg gcgaagcccc gagtgagcga ggaagcacca
181 tggaacagta ctcataaatt ctgcatacgc tcaatgcctg caaaatcacc tcccctaggg
241 gttatccact tatccacggg gatattttta taagatcttt tttaatagtt gttagatctt
301 tatttttaga atgcccacag gcctttatcc atgcgggttc cagagaggga tctgtgacaa
361 aacgcccttt cagtgtgaca aatcaccctc aaatagccgc cctttctgtg acaagttgcc
421 cttaaccctg tgacaaattg ccctcaggaa gcgttgcttt tcacaaagtt atccatccct
481 gttgactctg ttttatgaag tgtgacaatc ttaaagtctg tcacacttca catggacctg
541 tcatggcgga aatagcggtt ataaaccata aaaaacgtaa aaatagcccg cggattgtcc
601 agtcaaatga gctgactgag gcggcatata gtctctccag ggatcaaaag cgtctgctgt
661 atctgttcgt tcaccagatc agaaaatccg acggctccct gcaggaacat gacggcatct
721 gcgaaattca cgttgctaaa tacgctgaaa cattcgggtt gacctccgct gaagccagta
781 aggatatacg acaggcttta aaaggttttg cgggtaagga agtggttttc tatcgccctg
841 aagaggatgc cggcgatgaa aaagggtatg aatcctttcc ctggtttatt aaacgtgcgc
901 acagcccatc cagagggctt tacagcgtac atatcaaccc atatctgatt cccttcttca
961 tcgggttaca gaaccggttt acgcagttcc ggctcagtga aacaaaagag attaccaatc
1021 cgtacgccat gcgtttatac gaatctctgt gccagtaccg taaacctgat ggctcaggtg
1081 tcgtgtccct gaaaatcgac tggatcatgg aacgctacca gctacctcaa agttaccagc
1141 gtatgccgga ctttcgccgc cgtttcctga aggcaagtgt tgacgagatc aacagccgga
1201 caccaatgcg cctttcttac atcgagaaaa agaaaggccg ccagacgacg catatcgtat
1261 tttccttccg tgatataacc tccatgacga ttgaatagtt gagggttatc tgtcacagat
1321 ttaagggtgg ttcgtcacat ttgatctgga aggttgaggg ttatctgtca cagatttaag
1381 ggtggttcgt cacatttgat ctggaaggtt gagggtgatt tgtcacagat ttaagggtgg
1441 ttcgtcacat ttgatctgga aggttgaggg tgatttgtca cagttttgct gttccctccc
1501 tggggaa
//