LOCUS X53373 5741 bp DNA linear ENV 18-APR-2005
DEFINITION Unclassified alcalophilic gram-positive bacteria DSM 5853 amyA180
gene for exo-maltopentaohydrolase (amylase).
ACCESSION X53373
VERSION X53373.1
KEYWORDS amyA gene; amylase; exo-maltopentaohydrolase.
SOURCE uncultured bacterium
ORGANISM uncultured bacterium
Bacteria; environmental samples.
REFERENCE 1 (bases 1 to 5741)
AUTHORS Candussio A.
JOURNAL Submitted (30-MAY-1990) to the INSDC. Candussio A., Lehrstuhl fuer
Mikrobiologie der Universitaet Muenchen, Maria-Ward-Str. 1a, D-8000
Muenchen 19, FRG.
REFERENCE 2 (bases 1 to 5741)
AUTHORS Candussio A., Schmid G., Boeck A.
TITLE Biochemical and genetic analysis of a maltopentaose-producing
amylase from an alkaliphilic gram-positive bacterium
JOURNAL Eur. J. Biochem. 191(1), 177-185(1990).
PUBMED 1696201
FEATURES Location/Qualifiers
source 1..5741
/organism="uncultured bacterium"
/environmental_sample
/strain="UGO163/26"
/mol_type="genomic DNA"
/db_xref="taxon:77133"
regulatory 172..177
/note="-35 region"
/regulatory_class="promoter"
regulatory 196..201
/note="-10 region"
/regulatory_class="promoter"
precursor_RNA 212..>212
/note="transcript"
CDS 297..5351
/transl_table=11
/product="precursor polypeptide (AA -37 to 1647)"
/db_xref="GOA:Q03658"
/db_xref="InterPro:IPR003961"
/db_xref="InterPro:IPR005085"
/db_xref="InterPro:IPR006047"
/db_xref="InterPro:IPR013783"
/db_xref="InterPro:IPR017853"
/db_xref="InterPro:IPR031965"
/db_xref="InterPro:IPR036116"
/db_xref="InterPro:IPR041624"
/db_xref="UniProtKB/TrEMBL:Q03658"
/protein_id="CAA37453.1"
/translation="MKQQLNRVISIVLCLIVMLSVFESTIMLLPGSVEVKGQEYRELN
QLENKPFSWDNANVYFVLTDRFYNGNTSNDNSYGRPQIDAWGTNIGTFHGGDIKGLTK
KLEEGYFTDLGTNAIWISAPWEQMHGWVGGKDGDFAHYGYHGYYGLDFTAMDQNMGTI
DEMREFVDLAHSLGIRVVLDIVMNHVGYPTIVDMHEFGFGDTGGLPRDWTPNQAQGQN
WHTHNDIMNKDNEAAWANWWGSDWIRADETAGYDNCGGSEQTMCIGFLPDIKTEVTTG
VDLPPILRNKWNDQASGYEDWFVPAAEPYRQDLNIAPKDYLIKWITSWVEEFGIDGFR
VDTAKHVEIERWAELKNEAEVALQTWRENNPDKPGANWDDNFWMTAEVFGHGLGKSEY
FDFGFDSVINFEFQNANFNNLEGLFSRYANSINTDPDFNMLSYVSSHDTKLYSRDDLI
QAGTALLLLPGGVQVFYGDETARPLGDGGSDPEQGTRSSMNWANINQNVLSHWQKLGQ
FRNNHIAIGAGAHQKLSDSPYTFARTYESDDIVDEVVVATGAQGTTAVTVEGVFEDGT
VVRDAYTGDETTVTKGTATFTAGTQGIILIENTAEPVTNLPIVSATPGNSSFRTDDIT
ITLNVDRADMGKYTLDGSDPADGLTFMDGEEIVIGADMEFDETATLRLYAENENGIRT
RSYTYRKVDPDALLEVYFKKPADWGTPHIYYYDTFPEEPEVTWTTAPEMTLVEDDWYV
YVFENAESANIIFKDSSGKQIPGPNEPGFFIDQIGWYDGVKWLDSDPFEREPKEPATT
PKNLSVVNVTETTVTFEWDQSDGYVVEYEILRDEDVVASTIRTTFTDEDLNPDTTYTY
SVVAVGEGGQKSAPSEALKVTTLEENDEPKEPAEAPENLRIADITDTTVTINWNASNG
YVTGYEVLRDGVVIGETTRTTFIDTGLDADRTYTYTIVALGDGGQKSDPSEALEVTTQ
EKPEGNLVTIYYKKGFDTPYMHYRPEGGEWTIVPGIRMEESEIAGYSKLTVDIREASK
LEVAFNNGRGAWDSDQENNYLFEPGVHTYIPSHEGRGEIIPGKPGAPIDGNKVTIYYQ
NGFDTPYVHYRPEGGNWTNAPGLKMEDSEFASYSRLTLDIGEANRAEVAFNNGRGLWD
SDNENNYFFNIGDNTYIPGKNGSAGEIYGGKPRPPLVGNEVIIYYKNGFDTPYVHYRP
EGGTWTNAPGIKMDKSEIAGYSKITLDIGRADRVEVAFNDGRGAWDSDNERNYLFVVG
NNTYEPGINGAPGQVKHGVLPDDGEDPGDIEDPDHTSPSKPTDLTAIATAHTVSLSWT
ASADDVEVAGYKIYRDGVEIGVTESTTYTDSGLTAETTYSYMVQAYDTSNNFSALSDE
LTIETAEKTGVDPGGDMPYSTNPSFGKKVTTPITIDGVNDGEWTDDMLIAIGMAGDDP
RSLGDNWSMHETPMDLTHLWGAWDHEYLYLAWQYVDVTDIIDPANAGSSAGTTISQMD
MPQTIAIDTIPEQGATHDMWGKNGGESLWGGPDLPDYQLNIASNMFHSGYISRAVDGV
FPVDDGGINYKTGEEAGITVKFSKGKGYSTLWGVLDADDAVDPSKLVNFTELAHDSTR
DTFYEAKIPLAAIGNPDIENERIGVMIHQGEFSPMDTLPNDPATSDTPGVSESNSPLE
WEDIDLLTVPFARIGQ"
sig_peptide 297..407
/note="signal peptide (AA -37 to -1)"
mat_peptide 408..5348
/note="exo-maltopentaohydrolase (AA 1 to 1647)"
regulatory 5360..5385
/note="put. hairpin loop"
/regulatory_class="terminator"
BASE COUNT 1841 a 942 c 1355 g 1603 t
ORIGIN
1 ggtaccgcct atctcagtgt gtgaaagcta tgcatcaaaa tacctactcc atgagcgttt
61 cttcaacacg aatctacttt atttaatatt attcataacg aaacatcaga aaatattgtt
121 attacctaaa ttccttgttt ttgtctttta atgttggtca atgttctatg gttgtgctaa
181 taaaaatgtt aacgctttct caggaggcta tatgagaggg gtgatgtctg ctaaacaata
241 aggattcatc aacaccatgg ttataaaaaa ttaaagattg aaaggaggaa aaggtaatga
301 agcaacagct taatcgcgtg ataagtatcg tattatgttt aattgtcatg ctctcggtgt
361 ttgaaagtac tattatgtta ttaccaggtt cagtagaggt aaaaggccaa gagtatcgag
421 aactaaatca gctagaaaat aaaccttttt catgggataa tgcaaacgtt tactttgtgt
481 taaccgatcg tttttacaat ggaaatacaa gtaatgataa ttcttatggg agaccgcaaa
541 tagatgcttg gggtacaaac attggtactt tccatggcgg ggacataaaa ggattaacaa
601 agaaattgga agaaggttac tttacagacc taggtacaaa tgccatatgg atatctgctc
661 catgggaaca aatgcatggc tgggttggtg ggaaagatgg tgattttgct cactatggct
721 atcatggtta ctatggatta gattttacgg ctatggatca gaatatgggt acaattgatg
781 aaatgcgtga atttgttgac cttgcacatt cattaggcat tagagttgtt ctcgacattg
841 ttatgaatca cgttggctat ccaacgatcg ttgacatgca tgaatttggt tttggtgata
901 ctggaggact tccaagagat tggacaccta atcaagcaca gggccaaaat tggcatacac
961 ataatgacat tatgaataaa gacaatgaag cagcttgggc gaattggtgg ggaagtgact
1021 ggattcgtgc cgatgaaaca gccgggtatg acaattgtgg tggcagcgaa cagacaatgt
1081 gtatagggtt cttaccagat attaaaacgg aagtaaccac aggtgttgat ttaccaccga
1141 tattgagaaa caagtggaat gatcaagcta gtggctatga agattggttt gttccagcag
1201 ctgaacctta tcgtcaagat ttaaacattg ctccgaaaga ttatttgatc aaatggatta
1261 cttcatgggt tgaggaattc ggtattgatg gattccgtgt tgatacagca aagcatgtag
1321 agattgagcg atgggctgaa ttgaagaatg aagcggaagt agcacttcaa acatggcgag
1381 aaaataaccc agataagccc ggtgctaatt gggatgataa tttctggatg acagcagaag
1441 tatttggaca tggtcttggg aaaagcgagt attttgattt tggtttcgat tctgtgatta
1501 attttgaatt ccagaatgca aacttcaata atttagaagg tttattttct agatatgcaa
1561 attcaattaa cactgaccct gatttcaaca tgttaagtta tgtttcatct catgatacaa
1621 agctttatag tagagatgac cttattcagg caggtacagc attactatta cttccaggtg
1681 gcgttcaagt gttttatggc gatgaaacag ctcgaccatt aggggatggt ggttctgatc
1741 ctgagcaagg tacgcgttca tcgatgaatt gggctaatat taatcagaat gtactctctc
1801 attggcaaaa acttggtcaa ttcagaaata atcacatagc tattggtgcg ggagcgcatc
1861 agaagttatc tgatagtccg tatacgtttg cgcgtacgta tgaatcagac gatatagttg
1921 atgaagtcgt cgttgcaact ggggcccaag gaacaacagc tgttactgta gaaggtgttt
1981 ttgaagatgg gacagttgtt cgagatgctt atactggtga tgagacaaca gtaactaaag
2041 ggacagcaac atttactgct ggaacacaag gtattattct aatcgaaaat acagctgagc
2101 cagttactaa tttgccgatc gtttcagcaa cacctggtaa tagttctttt aggacagatg
2161 acataacaat cacgctaaat gttgatcgag cggatatggg gaagtataca cttgatggaa
2221 gtgatccagc agatggccta acgtttatgg atggagaaga aattgtcatt ggtgctgata
2281 tggagtttga tgaaacagca acattgagac tctatgcaga aaatgaaaat ggcataagaa
2341 caaggagtta cacatatagg aaggtagatc cagatgcgtt acttgaagta tattttaaga
2401 aaccagcgga ttggggaaca ccacatatat attactatga tacatttcca gaggagccgg
2461 aagtcacttg gactacagct ccagagatga cattagtaga ggatgattgg tatgtatatg
2521 tttttgaaaa tgctgaaagt gccaatataa tatttaagga ttcttcagga aaacaaattc
2581 caggtccaaa tgaaccagga ttcttcattg atcagattgg ttggtacgat ggcgtaaagt
2641 ggcttgattc agatcctttt gaaagggaac ctaaagagcc tgcgacaaca cctaagaacc
2701 taagtgttgt taatgtaact gaaactactg taacatttga gtgggaccaa tctgatggtt
2761 atgtcgttga atacgagatt ttacgtgatg aggatgttgt tgcttcaact attcgtacaa
2821 catttacgga tgaagacctt aatccagata caacctacac ttattctgtc gtagctgttg
2881 gagaaggcgg gcagaaatcc gccccaagtg aagcgttaaa agtgactaca ttagaagaaa
2941 atgatgaacc taaggaaccg gctgaggcgc cagaaaattt acgtatagct gatataacag
3001 atacaacagt tacaatcaac tggaatgcat ctaatggtta cgtaacagga tatgaggttc
3061 tgcgtgatgg tgtggttatt ggcgaaacaa cacggacaac attcatagat actggattag
3121 atgctgatag gacctatacg tatacgattg ttgctctcgg agatggcggg caaaagtctg
3181 atccgagcga agcgttagaa gtaacaactc aagaaaaacc agaaggaaat ctagtaacaa
3241 tatactataa aaaaggcttt gataccccat atatgcatta tcgtccggaa ggtggagagt
3301 ggacgatcgt tccaggaatt agaatggaag aatcagaaat agcaggctat agtaagttaa
3361 ccgttgatat tcgggaagca agcaagttgg aagtagcctt taataatgga cgtggggctt
3421 gggatagtga tcaagagaat aattatttat ttgagccagg tgttcatacg tacattccga
3481 gtcatgaagg aagaggagag attattccag gtaaaccagg agcaccaatc gatggtaata
3541 aagtgacgat ttactatcaa aatggctttg atacgccgta tgttcattac cgcccagaag
3601 gcggaaattg gaccaacgcc ccaggattaa aaatggaaga ttcagagttt gcaagttata
3661 gtaggttaac gcttgatatt ggtgaagcta atcgtgcaga agtggctttc aataacggac
3721 gcggcctttg ggatagtgat aatgaaaata attatttctt caatattggc gataacactt
3781 atataccagg aaaaaacggt tcagctggag agatttatgg aggtaagcca agaccaccat
3841 tagtaggaaa tgaagtaatc atttattata aaaatggttt tgatacaccg tatgttcatt
3901 atcgtccaga aggtggtacg tggacaaatg caccaggaat aaaaatggat aagtcagaaa
3961 tagcaggtta cagtaaaata acgcttgata ttggtcgcgc agatcgagta gaagtagcct
4021 ttaatgacgg tcgtggtgca tgggatagtg ataacgaacg taattatctc tttgtagtcg
4081 gtaacaatac ttatgaacca ggaattaacg gcgcacctgg tcaggtgaaa catggcgtgt
4141 tacctgatga tggagaagat ccgggagata ttgaagaccc tgatcatacc tccccttcaa
4201 agccgactga tttaacagca atagctactg ctcatactgt ttcattaagc tggacagctt
4261 cagcagacga tgtagaagta gctgggtaca aaatttatcg agatggtgtg gaaatcggtg
4321 ttactgaatc aacaacttat acggattcag gcttaacggc agaaacaacg tatagctata
4381 tggtacaagc ttatgatact tctaataatt tctcggcatt aagtgatgaa ctgacaattg
4441 aaaccgccga gaaaacgggt gttgatccag gaggggatat gccttattcc acgaatccat
4501 cgtttggtaa gaaggtaaca acgccaatca caattgatgg tgttaatgac ggggaatgga
4561 cagatgatat gttgattgca attggtatgg ctggtgacga cccacgttcg ctcggggaca
4621 attggtctat gcatgaaaca ccaatggacc ttactcacct atggggagca tgggaccatg
4681 agtacttgta tcttgcttgg caatatgtag atgtaacaga tattattgac ccagctaacg
4741 caggctcatc agctggtacc acaattagcc agatggatat gccacaaacc attgcaattg
4801 ataccatccc agagcaaggt gcaacacatg atatgtgggg gaaaaatggt ggtgaatcac
4861 tttggggagg accagattta cctgattacc aactaaatat cgcatctaat atgttccatt
4921 caggctatat ttctagagca gttgatggtg tatttcctgt tgacgatgga ggaataaatt
4981 ataaaacggg tgaggaagca ggaattacag taaagttttc taaaggtaaa gggtattcaa
5041 cattgtgggg ggtgttagat gctgatgatg cagttgatcc tagtaaactt gtgaacttca
5101 ccgagcttgc ccatgattca acacgagata ctttttatga agcaaagatt cctttagctg
5161 caattggtaa tcccgacatt gaaaatgaac gcattggtgt catgattcat caaggtgaat
5221 tttcgccgat ggacacgcta ccgaatgacc ccgcaacatc cgatacacca ggtgtgagtg
5281 aatcaaattc gccattagaa tgggaagaca ttgacctgtt aacagtgcca tttgcaagaa
5341 ttggccaata attatgaaat aagccggcat gagtctatgc tggctttttg tacggctggc
5401 agttgcacat gcagagacga cactgtggtg taacagctag atgacagtaa atcattggca
5461 ttccagatgg tcggaataaa agacggattt gtgtatatag taactctatt gatgaagttt
5521 tcccgttagt tccatcctca gattatcaat catcaataat aggtggtttc atggattgct
5581 taggaaatat cgaggatgac ctatttactg ttcactaaat ctgattagag tttattggta
5641 tcaaaaaagc gttatttctt caaagcaaga agcaggcaac tgggattatc tacatttccc
5701 aatgatatga aaaatgctgt taaaaaagat ggttgaatat t
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